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Strain Name:
C57BL/6J-MtgxR6894Btlr/Mmmh
Stock Number:
044988-MU
Citation ID:
RRID:MMRRC_044988-MU
Other Names:
R6894 (G1)
Major Collection:

Strain Information

Eomes
Name: eomesodermin
Synonyms: Tbr2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 13813
HGNC: HGNC:3372
Homologene: 3971
Ntrk1
Name: neurotrophic tyrosine kinase, receptor, type 1
Synonyms: TrkA, Tkr
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18211
HGNC: HGNC:8031
Homologene: 1898
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Cltc
Name: clathrin heavy chain
Synonyms: CHC
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 67300
HGNC: HGNC:2092
Homologene: 3572
Atr
Name: ataxia telangiectasia and Rad3 related
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 245000
HGNC: HGNC:882
Homologene: 96916
Kat7
Name: K(lysine) acetyltransferase 7
Synonyms: Hbo1, Hboa, Myst2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217127
Homologene: 5134
Baz2a
Name: bromodomain adjacent to zinc finger domain, 2A
Synonyms: Tip5, Walp3, C030005G16Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 116848
VEGA: 10
HGNC: HGNC:962
Homologene: 8393
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 58,911,733 bp
  • T to A, chromosome 1 at 88,701,375 bp
  • A to T, chromosome 1 at 123,336,864 bp
  • T to C, chromosome 1 at 150,436,847 bp
  • A to T, chromosome 2 at 25,295,817 bp
  • A to T, chromosome 2 at 76,908,190 bp
  • A to T, chromosome 2 at 86,332,951 bp
  • A to G, chromosome 2 at 89,303,493 bp
  • A to T, chromosome 2 at 120,303,596 bp
  • C to A, chromosome 2 at 151,473,307 bp
  • A to T, chromosome 3 at 87,782,802 bp
  • A to G, chromosome 3 at 88,982,991 bp
  • G to A, chromosome 3 at 103,044,656 bp
  • T to G, chromosome 4 at 32,748,614 bp
  • G to A, chromosome 4 at 42,850,291 bp
  • T to A, chromosome 4 at 123,483,687 bp
  • G to C, chromosome 5 at 136,697,971 bp
  • A to T, chromosome 5 at 142,760,049 bp
  • G to A, chromosome 5 at 146,504,732 bp
  • A to T, chromosome 5 at 146,504,733 bp
  • G to A, chromosome 5 at 146,754,539 bp
  • A to C, chromosome 6 at 18,055,521 bp
  • G to A, chromosome 6 at 48,930,662 bp
  • A to T, chromosome 6 at 56,882,973 bp
  • A to T, chromosome 6 at 66,553,361 bp
  • A to C, chromosome 6 at 69,858,651 bp
  • A to T, chromosome 6 at 85,808,522 bp
  • T to C, chromosome 6 at 122,591,242 bp
  • T to G, chromosome 7 at 30,125,836 bp
  • A to G, chromosome 7 at 44,633,512 bp
  • C to T, chromosome 7 at 107,815,064 bp
  • T to A, chromosome 7 at 109,336,062 bp
  • G to T, chromosome 7 at 109,901,118 bp
  • T to C, chromosome 7 at 122,048,224 bp
  • A to G, chromosome 7 at 137,387,388 bp
  • T to A, chromosome 8 at 3,853,569 bp
  • T to C, chromosome 8 at 4,105,477 bp
  • G to A, chromosome 8 at 22,560,593 bp
  • A to G, chromosome 8 at 23,157,616 bp
  • T to C, chromosome 9 at 15,997,776 bp
  • A to G, chromosome 9 at 18,495,576 bp
  • T to A, chromosome 9 at 35,646,673 bp
  • T to A, chromosome 9 at 95,927,197 bp
  • C to G, chromosome 9 at 118,481,285 bp
  • T to C, chromosome 9 at 123,700,933 bp
  • A to T, chromosome 10 at 9,784,361 bp
  • A to G, chromosome 10 at 18,169,380 bp
  • A to T, chromosome 10 at 51,716,039 bp
  • G to T, chromosome 10 at 79,169,604 bp
  • G to T, chromosome 10 at 128,123,581 bp
  • T to C, chromosome 10 at 130,034,309 bp
  • T to C, chromosome 11 at 49,160,359 bp
  • A to G, chromosome 11 at 53,436,819 bp
  • A to G, chromosome 11 at 59,132,682 bp
  • G to T, chromosome 11 at 59,373,024 bp
  • A to T, chromosome 11 at 69,484,260 bp
  • A to G, chromosome 11 at 78,194,787 bp
  • A to T, chromosome 11 at 86,712,602 bp
  • A to T, chromosome 11 at 95,284,084 bp
  • G to T, chromosome 11 at 105,396,961 bp
  • T to A, chromosome 12 at 81,987,973 bp
  • T to C, chromosome 12 at 98,715,181 bp
  • A to G, chromosome 13 at 32,722,015 bp
  • T to C, chromosome 13 at 48,590,638 bp
  • A to G, chromosome 14 at 26,735,749 bp
  • T to C, chromosome 15 at 8,187,368 bp
  • A to G, chromosome 15 at 67,111,346 bp
  • T to C, chromosome 15 at 74,762,805 bp
  • T to C, chromosome 15 at 95,346,887 bp
  • T to C, chromosome 15 at 101,053,973 bp
  • A to T, chromosome 16 at 10,644,854 bp
  • G to A, chromosome 16 at 11,088,145 bp
  • A to T, chromosome 16 at 16,783,938 bp
  • A to G, chromosome 16 at 59,303,779 bp
  • A to T, chromosome 16 at 79,075,814 bp
  • T to A, chromosome 17 at 56,188,321 bp
  • T to A, chromosome 17 at 78,626,758 bp
  • C to T, chromosome 18 at 34,695,657 bp
  • G to C, chromosome 19 at 10,738,839 bp
  • C to A, chromosome 19 at 30,624,774 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6894 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044988-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.