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Strain Name:
C57BL/6J-MtgxR6899Btlr/Mmmh
Stock Number:
044993-MU
Citation ID:
RRID:MMRRC_044993-MU
Other Names:
R6899 (G1)
Major Collection:

Strain Information

Dap3
Name: death associated protein 3
Synonyms: 4921514D13Rik, DAP-3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 65111
HGNC: HGNC:2673
Homologene: 3404
Tfpi
Name: tissue factor pathway inhibitor
Synonyms: A630013F22Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 21788
Homologene: 4579
Nup210l
Name: nucleoporin 210-like
Synonyms: 4930548O11Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 77595
Homologene: 28122
Oxsr1
Name: oxidative-stress responsive 1
Synonyms: 2810422B09Rik, 2210022N24Rik, Osr1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 108737
HGNC: HGNC:8508
Homologene: 31288
Fzd8
Name: frizzled class receptor 8
Synonyms: Fz8, mFZ8
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 14370
VEGA: 18
HGNC: HGNC:4046
Homologene: 40606
Washc4
Name: WASH complex subunit 4
Synonyms: A230046K03Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 319277
VEGA: 10
Homologene: 123926
Psat1
Name: phosphoserine aminotransferase 1
Synonyms: PSA, D8Ertd814e, EPIP
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 107272
Homologene: 6973
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 33,746,831 bp
  • A to G, chromosome 1 at 93,750,969 bp
  • A to G, chromosome 1 at 118,675,575 bp
  • A to G, chromosome 1 at 128,413,746 bp
  • A to G, chromosome 1 at 131,275,762 bp
  • T to A, chromosome 2 at 5,917,965 bp
  • C to T, chromosome 2 at 14,389,541 bp
  • G to A, chromosome 2 at 26,400,048 bp
  • C to T, chromosome 2 at 84,444,809 bp
  • T to A, chromosome 2 at 150,594,308 bp
  • C to T, chromosome 3 at 66,994,649 bp
  • A to G, chromosome 3 at 88,933,600 bp
  • A to G, chromosome 3 at 90,167,897 bp
  • A to T, chromosome 3 at 100,149,901 bp
  • A to G, chromosome 4 at 88,592,063 bp
  • A to T, chromosome 4 at 104,786,874 bp
  • A to T, chromosome 4 at 116,604,333 bp
  • G to A, chromosome 4 at 135,803,292 bp
  • A to G, chromosome 5 at 52,368,708 bp
  • A to G, chromosome 5 at 122,248,956 bp
  • T to A, chromosome 5 at 127,551,680 bp
  • T to C, chromosome 6 at 86,628,760 bp
  • T to A, chromosome 6 at 134,710,210 bp
  • T to C, chromosome 7 at 7,241,642 bp
  • G to T, chromosome 7 at 25,360,713 bp
  • A to T, chromosome 7 at 45,562,928 bp
  • TCCCAGG to T, chromosome 7 at 80,098,331 bp
  • A to G, chromosome 7 at 120,527,041 bp
  • A to G, chromosome 8 at 84,977,724 bp
  • T to A, chromosome 8 at 104,836,766 bp
  • T to C, chromosome 8 at 119,870,142 bp
  • A to T, chromosome 9 at 119,196,407 bp
  • C to T, chromosome 9 at 119,247,122 bp
  • T to C, chromosome 10 at 43,532,784 bp
  • C to A, chromosome 10 at 83,576,055 bp
  • G to A, chromosome 11 at 53,988,913 bp
  • T to A, chromosome 12 at 52,310,753 bp
  • A to G, chromosome 12 at 76,095,729 bp
  • G to A, chromosome 12 at 86,244,184 bp
  • A to T, chromosome 13 at 68,982,381 bp
  • T to C, chromosome 13 at 80,889,211 bp
  • A to C, chromosome 14 at 45,292,939 bp
  • C to T, chromosome 14 at 103,650,779 bp
  • A to G, chromosome 15 at 31,617,111 bp
  • G to A, chromosome 15 at 78,340,702 bp
  • A to G, chromosome 15 at 79,253,749 bp
  • A to G, chromosome 15 at 102,967,507 bp
  • A to T, chromosome 16 at 30,582,088 bp
  • T to C, chromosome 16 at 36,041,780 bp
  • T to A, chromosome 16 at 44,040,142 bp
  • A to G, chromosome 17 at 21,508,445 bp
  • G to A, chromosome 17 at 33,433,559 bp
  • T to A, chromosome 17 at 78,803,509 bp
  • T to A, chromosome 18 at 9,214,729 bp
  • A to G, chromosome 18 at 46,723,340 bp
  • A to G, chromosome 18 at 84,561,434 bp
  • C to T, chromosome 19 at 15,918,205 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6899 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044993-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.