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Strain Name:
B6.129S2-Dntttm1Gfn/Mmucd
Stock Number:
000045-UCD
Citation ID:
RRID:MMRRC_000045-UCD
Other Names:
TdT-/-, TdT0 , B6.129S2-Dntttm1Gfn/Mmcd

Strain Information

Dntttm1Gfn
Name: deoxynucleotidyltransferase, terminal; targeted mutation 1, Susan Gilfillan
Synonyms: TdT-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Knockout
Dntt
Name: deoxynucleotidyltransferase, terminal
Synonyms: Tdt
Type: Gene
Species: Mouse
Chromosome: 19
Alteration at locus: Knockout
NCBI: 21673
VEGA: 19
HGNC: HGNC:2983
Homologene: 3014
Genetic Alterations
A neo cassette was inserted into exon 4.
Genotype Determination
ES Cell Line
D3 derived from 129S2/SvPas
Phenotype
Dntt-/- mice don't express terminal deoxynucleotidyl transferase and therefore lack "N" nucleotide insertions at the junctions of Immunoglobulin (Ig) and T cell receptor (TCR) V(D)J rearrangements; consequently their Ig and TCR repertoires are significantly less diverse than those of wild-type mice.
Mammalian Phenotype Terms
Allelic Composition: Dntttm1Gfn/Dntttm1Gfn (Genetic Background: involves: 129S2/SvPas * C57BL/6 )

MeSH Terms
  • Animals
  • Base Sequence
  • DNA Nucleotidylexotransferase/genetics
  • DNA Nucleotidylexotransferase/metabolism
  • Gene Rearrangement, B-Lymphocyte
  • Gene Rearrangement, T-Lymphocyte
  • Genes, Immunoglobulin
  • Immunoglobulin Joining Region/genetics
  • Immunoglobulin Variable Region/genetics
  • Lymphocytes/immunology
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Mutation
  • Nucleotides/metabolism
  • Receptors, Antigen, T-Cell/genetics
  • Recombination, Genetic
  • DNA Nucleotidylexotransferase/deficiency
  • DNA Nucleotidylexotransferase/immunology
  • Humans
  • Receptors, Antigen, T-Cell/immunology
  • Receptors, Antigen, T-Cell, alpha-beta/immunology
  • Amino Acid Sequence
  • CD3 Complex/genetics
  • Cell Differentiation/immunology
  • DNA Nucleotidylexotransferase/physiology
  • Flow Cytometry
  • Mice, Mutant Strains
  • T-Lymphocytes/cytology
  • T-Lymphocytes/immunology
  • Thymus Gland/cytology
Strain Development
The targeting vector (Fig. 1 of PMID:8356452) was linearized and electroporated into D3 (129S2) embryonic stem cells as described in PMID:1909605. Of 151 Gancyclovir/G418-resistant D3 clones analyzed, one carrying the predicted integration was identified, and this was confirmed by extensive Southern blot analysis. It was expanded and injected into blastocysts, which were reimplanted into pseudopregnant females. Chimeras were crossed to C57BL/6 mice, and offspring carrying the mutation were intercrossed to produce homozygous knockout mice. The presence of the mutation was confirmed by Southern blot analysis of EcoRI/EcoRV/BglII-digested tail DNA with the use of a PCR-generated cDNA probe spanning exons 4 to 6.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Immunoglobulin and T cell receptor V(D)J recombination studies
  • Human disease
  • Immunology
Donor
Dr. S. Gilfillan, Washington University School of Medicine
Primary Reference

Gilfillan, S., A. Dierich, M. Lemeur, C. Benoist, and D. Mathis. Mice lacking TdT: mature animals with an immature lymphocyte repertoire. Science. 1993 Aug 27; 261(5125):1175-8. (Medline PMID: 8356452)

Gilfillan S, Benoist C, Mathis D. Mice lacking terminal deoxynucleotidyltransferase: adult mice with a fetal antigen receptor repertoire. Immunol Rev.1995 Dec;148:201-19. Review. (Medline PMID: 8825288)

Gilfillan S, Waltzinger C, Benoist C, Mathis D. More efficient positiveselection of thymocytes in mice lacking terminal deoxynucleotidyl transferase.Int Immunol. 1994 Nov;6(11):1681-6. (Medline PMID: 7865461)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Black
Eye
Black
MMRRC Breeding System
Sib-mating
Breeding Scheme(s)
  • Homozygous Female x Homozygous Male
Generation
N10+ prior to sibling inbreeding
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 6 months 6 months
Bred to Homozygosity
Yes
Average litter size
6
Recommended wean age
3 weeks
Average Pups Weaned
6

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
000045-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.