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Strain Name:
C57BL/6J-MtgxR6935Btlr/Mmmh
Stock Number:
045008-MU
Citation ID:
RRID:MMRRC_045008-MU
Other Names:
R6935 (G1)
Major Collection:

Strain Information

Mbd5
Name: methyl-CpG binding domain protein 5
Synonyms: 9430004D19Rik, C030040A15Rik, OTTMUSG00000012483
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 109241
Homologene: 81861
Ncam2
Name: neural cell adhesion molecule 2
Synonyms: RNCAM, R4B12 antigen, Ncam-2, Ocam
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 17968
HGNC: HGNC:7657
Homologene: 3336
Adcy10
Name: adenylate cyclase 10
Synonyms: soluble adenylyl cyclase, sAC, 4930431D04Rik, Sacy
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 271639
Homologene: 10188
Slc6a4
Name: solute carrier family 6 (neurotransmitter transporter, serotonin), member 4
Synonyms: 5-HTT, Htt, Sert
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 15567
Homologene: 817
Fbxo28
Name: F-box protein 28
Synonyms: 5730505P19Rik, 4833428J17Rik, Fbx28, D1Ertd578e
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 67948
Homologene: 9050
Xrcc5
Name: X-ray repair complementing defective repair in Chinese hamster cells 5
Synonyms: Ku80, Ku86
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 22596
VEGA: 1
Homologene: 40681
Rack1
Name: receptor for activated C kinase 1
Synonyms: p205, GB-like, Gnb2-rs1, Gnb2l1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14694
HGNC: HGNC:4399
Homologene: 4446
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 72,343,030 bp
  • T to C, chromosome 1 at 165,506,635 bp
  • C to T, chromosome 1 at 182,341,460 bp
  • A to T, chromosome 2 at 17,348,826 bp
  • A to G, chromosome 2 at 49,279,812 bp
  • T to C, chromosome 2 at 144,476,094 bp
  • T to C, chromosome 2 at 166,599,655 bp
  • T to G, chromosome 3 at 73,701,800 bp
  • A to G, chromosome 3 at 89,747,218 bp
  • T to G, chromosome 4 at 32,774,041 bp
  • A to T, chromosome 4 at 113,942,596 bp
  • A to T, chromosome 6 at 13,081,555 bp
  • A to T, chromosome 6 at 48,908,227 bp
  • G to A, chromosome 6 at 121,767,694 bp
  • A to G, chromosome 7 at 16,312,199 bp
  • G to C, chromosome 7 at 24,638,433 bp
  • C to A, chromosome 7 at 24,928,561 bp
  • T to C, chromosome 7 at 45,352,207 bp
  • T to A, chromosome 7 at 57,591,813 bp
  • A to T, chromosome 7 at 103,346,795 bp
  • A to T, chromosome 7 at 108,926,900 bp
  • A to T, chromosome 7 at 141,440,302 bp
  • T to A, chromosome 8 at 10,569,820 bp
  • CA to CAA, chromosome 8 at 13,734,865 bp
  • C to T, chromosome 8 at 23,108,231 bp
  • G to T, chromosome 9 at 38,292,117 bp
  • T to C, chromosome 9 at 56,619,865 bp
  • A to G, chromosome 9 at 79,700,500 bp
  • T to A, chromosome 9 at 110,639,391 bp
  • T to C, chromosome 10 at 59,896,503 bp
  • C to T, chromosome 10 at 76,504,253 bp
  • T to C, chromosome 11 at 48,803,495 bp
  • A to G, chromosome 11 at 49,430,998 bp
  • G to A, chromosome 11 at 69,421,741 bp
  • T to C, chromosome 11 at 77,027,168 bp
  • C to T, chromosome 11 at 80,337,496 bp
  • T to A, chromosome 11 at 100,698,406 bp
  • T to C, chromosome 12 at 113,140,593 bp
  • T to A, chromosome 14 at 30,912,702 bp
  • T to A, chromosome 14 at 31,379,516 bp
  • T to A, chromosome 14 at 35,811,907 bp
  • T to G, chromosome 14 at 55,863,878 bp
  • A to G, chromosome 15 at 10,319,302 bp
  • T to A, chromosome 16 at 81,526,991 bp
  • A to G, chromosome 19 at 16,872,619 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6935 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045008-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.