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Strain Name:
C57BL/6J-MtgxR6970Btlr/Mmmh
Stock Number:
045080-MU
Citation ID:
RRID:MMRRC_045080-MU
Other Names:
R6970 (G1)
Major Collection:

Strain Information

Adcy10
Name: adenylate cyclase 10
Synonyms: soluble adenylyl cyclase, sAC, 4930431D04Rik, Sacy
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 271639
Homologene: 10188
Rubcn
Name: RUN domain and cysteine-rich domain containing, Beclin 1-interacting protein
Synonyms: 1700021K19Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 100502698
Homologene: 15687
Jarid2
Name: jumonji and AT-rich interaction domain containing 2
Synonyms: Jmj, jumonji
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 16468
HGNC: HGNC:6196
Homologene: 31279
Zfp423
Name: zinc finger protein 423
Synonyms: Roaz, ataxia1, Zfp104, Ebfaz
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 94187
Homologene: 9010
Zmynd8
Name: zinc finger, MYND-type containing 8
Synonyms: 1110013E22Rik, 2010005I16Rik, ZMYND8, RACK7, 3632413B07Rik, Prkcbp1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228880
HGNC: HGNC:9397
Homologene: 32679
Ahdc1
Name: AT hook, DNA binding motif, containing 1
Synonyms: D030015G18Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230793
Homologene: 17144
Map3k4
Name: mitogen-activated protein kinase kinase kinase 4
Synonyms: MTK1, MAPKKK4, D17Rp17, RP17, D17Rp17e, Mekk4, T-associated sex reversal, Tas
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 26407
VEGA: 17
HGNC: HGNC:6856
Homologene: 31346
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 10,153,678 bp
  • G to T, chromosome 1 at 10,153,679 bp
  • T to G, chromosome 1 at 64,892,753 bp
  • G to A, chromosome 1 at 133,263,818 bp
  • T to G, chromosome 1 at 150,455,906 bp
  • T to A, chromosome 1 at 155,241,439 bp
  • A to G, chromosome 1 at 165,556,916 bp
  • G to A, chromosome 2 at 26,430,486 bp
  • C to T, chromosome 2 at 32,051,798 bp
  • T to C, chromosome 2 at 35,118,137 bp
  • T to G, chromosome 2 at 76,895,423 bp
  • T to A, chromosome 2 at 91,772,600 bp
  • A to G, chromosome 2 at 104,788,130 bp
  • A to C, chromosome 2 at 121,378,014 bp
  • G to A, chromosome 2 at 156,167,584 bp
  • C to T, chromosome 2 at 165,875,750 bp
  • A to G, chromosome 2 at 181,586,348 bp
  • A to G, chromosome 3 at 38,981,775 bp
  • A to T, chromosome 3 at 38,995,971 bp
  • A to T, chromosome 3 at 55,466,601 bp
  • C to A, chromosome 3 at 96,284,620 bp
  • A to T, chromosome 3 at 105,680,358 bp
  • A to G, chromosome 3 at 145,988,626 bp
  • T to A, chromosome 4 at 133,062,345 bp
  • G to A, chromosome 4 at 139,406,528 bp
  • A to G, chromosome 5 at 43,718,585 bp
  • A to G, chromosome 5 at 67,738,462 bp
  • A to C, chromosome 5 at 89,179,831 bp
  • A to G, chromosome 5 at 98,184,612 bp
  • G to A, chromosome 5 at 110,656,862 bp
  • A to G, chromosome 5 at 123,445,672 bp
  • A to G, chromosome 5 at 142,727,989 bp
  • T to C, chromosome 6 at 40,544,656 bp
  • A to G, chromosome 6 at 120,198,131 bp
  • T to A, chromosome 7 at 9,075,341 bp
  • T to A, chromosome 7 at 19,989,977 bp
  • C to T, chromosome 7 at 46,045,967 bp
  • T to C, chromosome 7 at 100,507,422 bp
  • T to C, chromosome 7 at 131,434,315 bp
  • G to T, chromosome 8 at 12,637,000 bp
  • T to C, chromosome 8 at 25,059,264 bp
  • A to T, chromosome 8 at 33,557,428 bp
  • C to T, chromosome 8 at 48,236,439 bp
  • G to A, chromosome 8 at 85,359,263 bp
  • A to G, chromosome 8 at 87,803,779 bp
  • T to G, chromosome 9 at 31,329,823 bp
  • T to A, chromosome 9 at 40,006,713 bp
  • T to C, chromosome 9 at 116,110,051 bp
  • G to A, chromosome 9 at 120,574,227 bp
  • T to C, chromosome 10 at 13,125,116 bp
  • A to T, chromosome 10 at 41,615,958 bp
  • C to T, chromosome 10 at 74,502,687 bp
  • A to G, chromosome 10 at 79,530,546 bp
  • A to G, chromosome 10 at 81,879,470 bp
  • A to G, chromosome 10 at 83,316,096 bp
  • G to T, chromosome 11 at 48,888,490 bp
  • G to A, chromosome 11 at 48,907,912 bp
  • A to G, chromosome 11 at 104,776,356 bp
  • T to G, chromosome 11 at 119,356,972 bp
  • G to A, chromosome 11 at 120,095,364 bp
  • A to G, chromosome 12 at 21,345,668 bp
  • G to T, chromosome 12 at 87,516,618 bp
  • T to A, chromosome 12 at 118,108,944 bp
  • C to T, chromosome 13 at 44,902,985 bp
  • A to T, chromosome 13 at 50,246,971 bp
  • C to T, chromosome 13 at 102,804,647 bp
  • A to G, chromosome 14 at 123,314,094 bp
  • T to C, chromosome 15 at 8,187,548 bp
  • G to A, chromosome 15 at 44,511,674 bp
  • A to G, chromosome 15 at 82,031,174 bp
  • T to A, chromosome 16 at 11,193,694 bp
  • G to T, chromosome 16 at 32,868,144 bp
  • A to T, chromosome 16 at 34,709,533 bp
  • C to A, chromosome 17 at 12,248,916 bp
  • G to A, chromosome 17 at 23,346,015 bp
  • A to C, chromosome 18 at 80,667,013 bp
  • T to A, chromosome 19 at 5,457,220 bp
  • G to T, chromosome 19 at 5,485,526 bp
  • A to G, chromosome 19 at 11,112,314 bp
  • T to A, chromosome 19 at 13,411,428 bp
  • C to T, chromosome 19 at 29,587,772 bp
  • G to T, chromosome 19 at 40,962,377 bp
  • C to A, chromosome 19 at 55,755,048 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6970 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045080-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.