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Strain Name:
C57BL/6J-MtgxR7014Btlr/Mmmh
Stock Number:
045115-MU
Citation ID:
RRID:MMRRC_045115-MU
Other Names:
R7014 (G1)
Major Collection:

Strain Information

Cdk6
Name: cyclin dependent kinase 6
Synonyms: Crk2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 12571
HGNC: HGNC:1777
Homologene: 963
Smyd1
Name: SET and MYND domain containing 1
Synonyms: Bop, 4632404M21Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 12180
Homologene: 7645
Zmym6
Name: zinc finger, MYM-type 6
Synonyms: D4Wsu24e, 9330177P20Rik, Zfp258
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 100177
Homologene: 5186
Pdzd2
Name: PDZ domain containing 2
Synonyms: 4930537L06Rik, LOC223364, A930022H17Rik, Pdzk3, Gm21706
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 68070
Homologene: 23393
Col6a1
Name: collagen, type VI, alpha 1
Synonyms: Col6a-1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 12833
HGNC: HGNC:2211
Homologene: 1391
Smarcad1
Name: SNF2 related chromatin remodeling ATPase with DExD box 1
Synonyms: D6Pas1, Etl1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 13990
Homologene: 5301
Rps6ka2
Name: ribosomal protein S6 kinase, polypeptide 2
Synonyms: Rsk3, Rps6ka-rs1, 90kDa, p90rsk, pp90rsk, D17Wsu134e
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 20112
VEGA: 17
Homologene: 100680
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 64,883,270 bp
  • A to T, chromosome 1 at 96,823,781 bp
  • T to C, chromosome 1 at 156,330,962 bp
  • A to T, chromosome 1 at 160,342,072 bp
  • C to G, chromosome 1 at 162,926,308 bp
  • T to A, chromosome 1 at 168,431,380 bp
  • A to G, chromosome 2 at 37,560,563 bp
  • C to A, chromosome 2 at 66,741,959 bp
  • A to G, chromosome 2 at 77,132,297 bp
  • A to T, chromosome 2 at 87,645,976 bp
  • T to C, chromosome 2 at 87,753,871 bp
  • A to G, chromosome 2 at 110,644,364 bp
  • A to C, chromosome 2 at 121,300,239 bp
  • A to T, chromosome 2 at 150,255,262 bp
  • C to A, chromosome 2 at 154,224,956 bp
  • G to T, chromosome 3 at 97,715,422 bp
  • C to T, chromosome 3 at 107,926,962 bp
  • T to A, chromosome 4 at 60,069,866 bp
  • T to A, chromosome 4 at 118,623,378 bp
  • T to A, chromosome 4 at 127,123,544 bp
  • CGG to CG, chromosome 4 at 141,918,291 bp
  • C to G, chromosome 5 at 3,473,152 bp
  • A to G, chromosome 5 at 101,894,909 bp
  • A to G, chromosome 5 at 107,508,276 bp
  • T to C, chromosome 5 at 109,053,423 bp
  • A to G, chromosome 5 at 131,466,123 bp
  • A to T, chromosome 5 at 149,630,400 bp
  • C to T, chromosome 6 at 21,772,919 bp
  • C to T, chromosome 6 at 24,519,191 bp
  • T to A, chromosome 6 at 30,450,503 bp
  • T to C, chromosome 6 at 65,052,670 bp
  • T to C, chromosome 6 at 71,238,627 bp
  • T to A, chromosome 6 at 87,902,340 bp
  • T to A, chromosome 6 at 108,431,498 bp
  • C to G, chromosome 6 at 116,403,543 bp
  • T to G, chromosome 6 at 116,403,544 bp
  • C to T, chromosome 6 at 121,861,125 bp
  • C to T, chromosome 7 at 27,578,773 bp
  • A to G, chromosome 7 at 30,454,574 bp
  • T to C, chromosome 7 at 55,919,493 bp
  • T to C, chromosome 7 at 104,483,336 bp
  • T to C, chromosome 7 at 119,451,645 bp
  • T to A, chromosome 8 at 4,754,234 bp
  • C to T, chromosome 8 at 83,042,663 bp
  • T to C, chromosome 8 at 83,610,255 bp
  • A to G, chromosome 9 at 14,464,179 bp
  • T to A, chromosome 9 at 18,658,236 bp
  • T to A, chromosome 9 at 20,300,663 bp
  • C to A, chromosome 9 at 36,582,558 bp
  • T to A, chromosome 9 at 36,909,667 bp
  • C to T, chromosome 9 at 37,233,887 bp
  • T to G, chromosome 9 at 71,725,134 bp
  • T to C, chromosome 9 at 72,891,985 bp
  • T to C, chromosome 10 at 19,712,710 bp
  • T to C, chromosome 10 at 28,789,707 bp
  • T to C, chromosome 10 at 76,721,443 bp
  • G to T, chromosome 10 at 79,950,884 bp
  • A to G, chromosome 11 at 104,693,422 bp
  • A to G, chromosome 11 at 115,620,966 bp
  • G to T, chromosome 11 at 116,134,915 bp
  • T to C, chromosome 11 at 118,212,651 bp
  • A to T, chromosome 12 at 11,091,687 bp
  • A to G, chromosome 12 at 55,045,942 bp
  • A to G, chromosome 13 at 21,555,938 bp
  • T to C, chromosome 14 at 41,002,494 bp
  • T to C, chromosome 15 at 12,372,561 bp
  • A to G, chromosome 15 at 12,372,975 bp
  • A to G, chromosome 16 at 8,661,005 bp
  • A to G, chromosome 16 at 17,297,067 bp
  • T to A, chromosome 16 at 18,624,909 bp
  • A to T, chromosome 16 at 19,515,456 bp
  • T to A, chromosome 16 at 35,960,063 bp
  • A to T, chromosome 16 at 36,049,932 bp
  • C to T, chromosome 17 at 7,255,932 bp
  • T to A, chromosome 17 at 8,566,472 bp
  • T to A, chromosome 17 at 32,049,427 bp
  • C to A, chromosome 17 at 32,361,866 bp
  • C to A, chromosome 17 at 32,415,930 bp
  • T to A, chromosome 18 at 47,298,217 bp
  • T to A, chromosome 18 at 75,437,208 bp
  • A to T, chromosome 19 at 11,548,583 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7014 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045115-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.