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Strain Name:
C57BL/6J-MtgxR7065Btlr/Mmmh
Stock Number:
045161-MU
Citation ID:
RRID:MMRRC_045161-MU
Other Names:
R7065 (G1)
Major Collection:

Strain Information

Pax3
Name: paired box 3
Synonyms: Pax-3, Splchl2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18505
HGNC: HGNC:8617
Homologene: 22494
Gpm6a
Name: glycoprotein m6a
Synonyms: M6A, Gpm6
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234267
HGNC: HGNC:4460
Homologene: 3864
Scn3a
Name: sodium channel, voltage-gated, type III, alpha
Synonyms: LOC381367, Nav1.3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20269
Homologene: 56005
Chmp4b
Name: charged multivesicular body protein 4B
Synonyms: 2010012F05Rik, Snf7-2, chromatin modifying protein 4B
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 75608
Homologene: 57102
Ehmt1
Name: euchromatic histone methyltransferase 1
Synonyms: 9230102N17Rik, KMT1D
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 77683
Homologene: 11698
Ctnna1
Name: catenin alpha 1
Synonyms: alpha(E)-catenin, alpha E catenin, Catna1, 2010010M04Rik, catenin (cadherin associated protein), alpha 1
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 12385
VEGA: 18
HGNC: HGNC:2509
Homologene: 1433
Fryl
Name: FRY like transcription coactivator
Synonyms: 2310004H21Rik, 2510002A14Rik, 9030227G01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 72313
Homologene: 103956
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to G, chromosome 1 at 58,731,209 bp
  • T to C, chromosome 1 at 78,194,011 bp
  • T to A, chromosome 1 at 93,304,895 bp
  • T to A, chromosome 1 at 140,086,402 bp
  • A to G, chromosome 1 at 151,700,107 bp
  • G to A, chromosome 2 at 4,566,112 bp
  • T to C, chromosome 2 at 24,840,697 bp
  • A to C, chromosome 2 at 65,464,855 bp
  • A to G, chromosome 2 at 69,953,705 bp
  • A to G, chromosome 2 at 76,798,112 bp
  • A to G, chromosome 2 at 85,430,179 bp
  • A to C, chromosome 2 at 86,146,001 bp
  • A to T, chromosome 2 at 88,831,386 bp
  • A to T, chromosome 2 at 91,155,057 bp
  • A to G, chromosome 2 at 91,511,580 bp
  • A to G, chromosome 2 at 120,718,921 bp
  • A to G, chromosome 2 at 154,691,242 bp
  • A to G, chromosome 3 at 96,868,432 bp
  • A to G, chromosome 3 at 122,262,671 bp
  • A to G, chromosome 3 at 151,759,792 bp
  • G to T, chromosome 4 at 53,074,233 bp
  • C to A, chromosome 4 at 138,800,604 bp
  • T to C, chromosome 4 at 149,202,525 bp
  • A to C, chromosome 5 at 15,620,423 bp
  • T to C, chromosome 5 at 73,090,756 bp
  • T to G, chromosome 6 at 73,087,562 bp
  • C to A, chromosome 6 at 91,783,569 bp
  • T to C, chromosome 6 at 136,720,766 bp
  • A to G, chromosome 7 at 25,385,178 bp
  • T to C, chromosome 7 at 29,103,643 bp
  • A to G, chromosome 7 at 43,998,962 bp
  • G to A, chromosome 7 at 108,613,059 bp
  • T to A, chromosome 7 at 140,763,993 bp
  • A to G, chromosome 8 at 24,711,675 bp
  • C to A, chromosome 8 at 55,037,458 bp
  • C to A, chromosome 8 at 128,460,712 bp
  • T to C, chromosome 9 at 13,818,381 bp
  • T to C, chromosome 9 at 21,090,294 bp
  • A to C, chromosome 9 at 42,543,831 bp
  • T to C, chromosome 9 at 95,485,659 bp
  • A to G, chromosome 9 at 96,179,056 bp
  • A to G, chromosome 9 at 104,041,990 bp
  • A to G, chromosome 9 at 115,266,156 bp
  • T to C, chromosome 10 at 22,067,298 bp
  • T to A, chromosome 11 at 9,292,595 bp
  • T to A, chromosome 11 at 78,336,739 bp
  • T to C, chromosome 11 at 83,016,968 bp
  • T to A, chromosome 12 at 8,952,472 bp
  • G to A, chromosome 12 at 36,187,708 bp
  • A to G, chromosome 12 at 58,268,794 bp
  • A to T, chromosome 13 at 54,991,083 bp
  • A to T, chromosome 13 at 67,442,282 bp
  • T to A, chromosome 14 at 32,172,900 bp
  • C to A, chromosome 14 at 34,595,877 bp
  • T to C, chromosome 14 at 52,141,193 bp
  • T to C, chromosome 15 at 68,181,120 bp
  • T to A, chromosome 16 at 44,355,535 bp
  • G to A, chromosome 17 at 3,159,211 bp
  • T to A, chromosome 17 at 24,327,751 bp
  • A to G, chromosome 17 at 34,622,855 bp
  • A to T, chromosome 18 at 35,152,616 bp
  • A to G, chromosome 18 at 37,021,626 bp
  • A to G, chromosome 18 at 65,195,969 bp
  • A to G, chromosome Y at 725,428 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7065 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045161-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.