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Strain Name:
C57BL/6J-MtgxR7101Btlr/Mmmh
Stock Number:
045193-MU
Citation ID:
RRID:MMRRC_045193-MU
Other Names:
R7101 (G1)
Major Collection:

Strain Information

Ppp1r16b
Name: protein phosphatase 1, regulatory subunit 16B
Synonyms: ANKRD4, Wdt4, C130078N17Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228852
Homologene: 9194
Odc1
Name: ornithine decarboxylase, structural 1
Synonyms: ODC
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 18263
VEGA: 12
HGNC: HGNC:8109
Homologene: 1906
Prrc2b
Name: proline-rich coiled-coil 2B
Synonyms: 5830434P21Rik, Bat2l
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227723
Homologene: 106649
Txn2
Name: thioredoxin 2
Synonyms: 2510006J11Rik, Trx2
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 56551
Homologene: 40849
Prex2
Name: phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2
Synonyms: 6230420N16Rik, C030045D06Rik, Depdc2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 109294
Homologene: 23523
Zfp638
Name: zinc finger protein 638
Synonyms: Np220, Zfml
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 18139
Homologene: 7447
Drosha
Name: drosha, ribonuclease type III
Synonyms: 1110013A17Rik, Etohi2, Rnasen
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 14000
Homologene: 8293
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 11,153,609 bp
  • G to T, chromosome 1 at 51,758,001 bp
  • G to A, chromosome 1 at 106,172,667 bp
  • G to A, chromosome 2 at 25,296,635 bp
  • A to G, chromosome 2 at 32,226,993 bp
  • A to T, chromosome 2 at 89,013,980 bp
  • T to C, chromosome 2 at 158,761,763 bp
  • A to T, chromosome 2 at 164,290,013 bp
  • A to T, chromosome 4 at 109,925,956 bp
  • A to G, chromosome 5 at 32,829,427 bp
  • C to T, chromosome 5 at 111,085,092 bp
  • C to A, chromosome 5 at 114,342,197 bp
  • C to T, chromosome 5 at 124,114,495 bp
  • G to A, chromosome 5 at 125,424,942 bp
  • C to A, chromosome 5 at 137,398,290 bp
  • T to A, chromosome 6 at 8,014,795 bp
  • G to T, chromosome 6 at 29,716,784 bp
  • T to C, chromosome 6 at 83,150,012 bp
  • T to C, chromosome 6 at 83,954,726 bp
  • C to G, chromosome 6 at 125,312,800 bp
  • A to T, chromosome 7 at 14,332,827 bp
  • A to G, chromosome 7 at 19,094,859 bp
  • G to T, chromosome 7 at 24,104,533 bp
  • A to T, chromosome 7 at 92,631,544 bp
  • AGGCGCAGAAACCCTGGC to AGGC, chromosome 7 at 141,634,450 bp
  • G to T, chromosome 8 at 13,013,579 bp
  • A to C, chromosome 8 at 35,482,623 bp
  • A to G, chromosome 8 at 40,755,401 bp
  • A to G, chromosome 8 at 84,997,051 bp
  • A to G, chromosome 8 at 109,732,500 bp
  • G to T, chromosome 8 at 122,895,455 bp
  • T to C, chromosome 8 at 123,906,227 bp
  • T to C, chromosome 9 at 37,772,991 bp
  • G to A, chromosome 9 at 38,539,670 bp
  • G to A, chromosome 9 at 86,507,669 bp
  • T to C, chromosome 9 at 104,165,022 bp
  • T to C, chromosome 10 at 13,247,178 bp
  • T to A, chromosome 10 at 28,761,426 bp
  • T to C, chromosome 10 at 70,930,653 bp
  • T to C, chromosome 10 at 75,751,567 bp
  • C to T, chromosome 10 at 77,751,333 bp
  • C to A, chromosome 10 at 77,862,360 bp
  • A to T, chromosome 10 at 88,440,312 bp
  • T to C, chromosome 10 at 128,121,187 bp
  • C to T, chromosome 10 at 128,382,380 bp
  • T to A, chromosome 11 at 23,426,183 bp
  • A to G, chromosome 11 at 58,502,553 bp
  • C to T, chromosome 11 at 75,490,400 bp
  • A to G, chromosome 12 at 17,547,318 bp
  • T to C, chromosome 12 at 118,068,145 bp
  • A to G, chromosome 13 at 50,472,898 bp
  • T to A, chromosome 13 at 67,390,812 bp
  • C to A, chromosome 13 at 93,356,054 bp
  • G to A, chromosome 13 at 112,495,373 bp
  • G to T, chromosome 14 at 8,511,171 bp
  • G to A, chromosome 14 at 16,283,445 bp
  • T to C, chromosome 14 at 21,775,374 bp
  • C to T, chromosome 14 at 32,960,820 bp
  • A to G, chromosome 14 at 56,589,973 bp
  • C to T, chromosome 15 at 12,865,067 bp
  • T to C, chromosome 15 at 42,523,569 bp
  • G to A, chromosome 15 at 77,926,678 bp
  • C to T, chromosome 16 at 21,218,518 bp
  • A to G, chromosome 17 at 19,589,088 bp
  • A to T, chromosome 17 at 20,030,096 bp
  • A to G, chromosome 17 at 25,163,468 bp
  • G to T, chromosome 17 at 44,275,292 bp
  • A to T, chromosome 17 at 52,905,010 bp
  • G to A, chromosome 17 at 56,294,522 bp
  • T to A, chromosome 18 at 36,681,538 bp
  • A to G, chromosome 18 at 77,766,870 bp
  • T to A, chromosome 19 at 40,972,638 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7101 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045193-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.