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Strain Name:
C57BL/6J-MtgxR7147Btlr/Mmmh
Stock Number:
045224-MU
Citation ID:
RRID:MMRRC_045224-MU
Other Names:
R7147 (G1)
Major Collection:

Strain Information

Sbf2
Name: SET binding factor 2
Synonyms: SBF2, 4833411B01Rik, mMTMH1, B430219L04Rik, Mtmr13
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 319934
HGNC: HGNC:2135
Homologene: 41810
Dlg1
Name: discs large MAGUK scaffold protein 1
Synonyms: B130052P05Rik, SAP97, Dlgh1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 13383
HGNC: HGNC:2900
Homologene: 20869
Trim13
Name: tripartite motif-containing 13
Synonyms: 3110001L12Rik, LEU5, Rfp2, RNF77
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 66597
VEGA: 14
HGNC: HGNC:9976
Homologene: 4234
Trim6
Name: tripartite motif-containing 6
Synonyms: D7Ertd684e, C430046K18Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 94088
Homologene: 14381
Srgap2
Name: SLIT-ROBO Rho GTPase activating protein 2
Synonyms: FBP2, 9930124L22Rik, Fnbp2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14270
Homologene: 52683
Arl8b
Name: ADP-ribosylation factor-like 8B
Synonyms: 3100002J04Rik, 2610313E07Rik, Arl10c
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 67166
Homologene: 10056
Ints14
Name: integrator complex subunit 14
Synonyms: 2010321M09Rik, Vwa9
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 69882
VEGA: 9
Homologene: 12774
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 125,872,501 bp
  • C to T, chromosome 1 at 131,310,594 bp
  • T to A, chromosome 1 at 181,001,819 bp
  • T to C, chromosome 2 at 82,258,187 bp
  • T to A, chromosome 3 at 26,133,360 bp
  • A to T, chromosome 3 at 27,150,090 bp
  • T to A, chromosome 3 at 86,104,277 bp
  • T to A, chromosome 3 at 100,883,894 bp
  • T to A, chromosome 4 at 25,213,777 bp
  • A to T, chromosome 4 at 98,961,417 bp
  • A to T, chromosome 4 at 118,484,413 bp
  • A to T, chromosome 4 at 123,810,115 bp
  • A to G, chromosome 4 at 135,346,826 bp
  • C to T, chromosome 5 at 49,961,245 bp
  • A to G, chromosome 5 at 134,657,341 bp
  • C to T, chromosome 6 at 3,567,750 bp
  • T to C, chromosome 6 at 4,624,187 bp
  • G to A, chromosome 6 at 29,758,449 bp
  • A to T, chromosome 6 at 34,746,296 bp
  • G to A, chromosome 6 at 108,815,015 bp
  • C to T, chromosome 6 at 118,465,002 bp
  • A to G, chromosome 7 at 4,550,782 bp
  • T to C, chromosome 7 at 45,033,850 bp
  • A to G, chromosome 7 at 74,504,294 bp
  • G to A, chromosome 7 at 104,225,570 bp
  • A to G, chromosome 7 at 104,564,066 bp
  • A to T, chromosome 7 at 110,447,061 bp
  • G to A, chromosome 7 at 110,804,852 bp
  • C to T, chromosome 7 at 112,323,603 bp
  • A to T, chromosome 7 at 120,813,015 bp
  • G to T, chromosome 7 at 145,308,391 bp
  • T to C, chromosome 8 at 45,250,146 bp
  • A to C, chromosome 8 at 46,105,196 bp
  • A to G, chromosome 8 at 75,057,267 bp
  • A to G, chromosome 8 at 81,902,771 bp
  • A to T, chromosome 9 at 44,355,082 bp
  • T to C, chromosome 9 at 54,416,729 bp
  • T to C, chromosome 9 at 64,983,985 bp
  • T to A, chromosome 9 at 81,927,596 bp
  • A to G, chromosome 9 at 109,789,323 bp
  • A to G, chromosome 9 at 123,568,951 bp
  • A to T, chromosome 10 at 5,249,340 bp
  • A to T, chromosome 10 at 22,181,280 bp
  • T to C, chromosome 11 at 5,873,095 bp
  • G to C, chromosome 11 at 49,059,308 bp
  • G to A, chromosome 11 at 62,605,969 bp
  • T to A, chromosome 11 at 118,094,929 bp
  • C to T, chromosome 12 at 16,733,427 bp
  • A to T, chromosome 12 at 105,808,638 bp
  • A to G, chromosome 13 at 21,811,989 bp
  • A to T, chromosome 13 at 24,882,563 bp
  • T to A, chromosome 13 at 59,718,214 bp
  • T to C, chromosome 13 at 119,766,543 bp
  • C to T, chromosome 14 at 55,779,725 bp
  • A to T, chromosome 14 at 61,604,631 bp
  • A to G, chromosome 14 at 78,511,465 bp
  • AAGCGGGCTGAGC to AAGC, chromosome 15 at 80,257,299 bp
  • A to G, chromosome 15 at 90,980,883 bp
  • A to T, chromosome 16 at 31,791,854 bp
  • T to A, chromosome 16 at 78,946,741 bp
  • G to A, chromosome 17 at 20,571,061 bp
  • A to T, chromosome 17 at 22,376,000 bp
  • C to T, chromosome 17 at 29,846,521 bp
  • T to C, chromosome 17 at 70,662,758 bp
  • T to A, chromosome 18 at 42,550,063 bp
  • T to C, chromosome 18 at 44,493,513 bp
  • C to T, chromosome 18 at 67,653,268 bp
  • T to C, chromosome 19 at 3,271,676 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7147 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045224-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.