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Strain Name:
C57BL/6J-MtgxR7114Btlr/Mmmh
Stock Number:
045244-MU
Citation ID:
RRID:MMRRC_045244-MU
Other Names:
R7114 (G1)
Major Collection:

Strain Information

Wrn
Name: Werner syndrome RecQ like helicase
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 22427
Homologene: 6659
Pomt1
Name: protein-O-mannosyltransferase 1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 99011
HGNC: HGNC:9202
Homologene: 68548
Plcb4
Name: phospholipase C, beta 4
Synonyms: C230058B11Rik, A930039J07Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18798
HGNC: HGNC:9059
Homologene: 8471
Arhgap12
Name: Rho GTPase activating protein 12
Synonyms: 2810011M08Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 75415
Homologene: 23089
Cep290
Name: centrosomal protein 290
Synonyms: Nphp6, b2b1454Clo, b2b1752Clo, Kiaa
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216274
VEGA: 10
Homologene: 77213
Helb
Name: helicase (DNA) B
Synonyms: D10Ertd664e
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 117599
Homologene: 50463
Fancl
Name: Fanconi anemia, complementation group L
Synonyms: 2010322C19Rik, gcd, Pog, Phf9, B230118H11Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 67030
Homologene: 9987
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 24,337,936 bp
  • T to A, chromosome 1 at 51,185,245 bp
  • T to C, chromosome 1 at 54,423,507 bp
  • T to C, chromosome 1 at 135,966,781 bp
  • A to T, chromosome 1 at 174,391,239 bp
  • C to A, chromosome 1 at 183,087,466 bp
  • T to A, chromosome 2 at 31,930,645 bp
  • T to C, chromosome 2 at 32,025,244 bp
  • C to T, chromosome 2 at 32,253,836 bp
  • G to A, chromosome 2 at 52,192,559 bp
  • A to C, chromosome 2 at 64,916,853 bp
  • G to T, chromosome 2 at 121,048,496 bp
  • T to A, chromosome 2 at 135,982,123 bp
  • C to T, chromosome 2 at 180,603,797 bp
  • C to T, chromosome 3 at 89,407,530 bp
  • A to G, chromosome 3 at 130,595,675 bp
  • T to A, chromosome 5 at 110,202,712 bp
  • T to C, chromosome 5 at 123,116,678 bp
  • T to C, chromosome 5 at 130,839,283 bp
  • T to A, chromosome 5 at 145,211,178 bp
  • T to A, chromosome 5 at 145,858,783 bp
  • T to C, chromosome 6 at 47,865,976 bp
  • C to T, chromosome 6 at 58,008,770 bp
  • T to G, chromosome 6 at 69,823,531 bp
  • T to C, chromosome 6 at 97,116,004 bp
  • A to T, chromosome 7 at 27,191,749 bp
  • AGCAGGTAATACATACCG to AG, chromosome 8 at 33,285,121 bp
  • T to C, chromosome 8 at 123,915,373 bp
  • C to T, chromosome 9 at 15,069,815 bp
  • T to C, chromosome 9 at 15,721,954 bp
  • G to T, chromosome 9 at 39,258,601 bp
  • G to T, chromosome 9 at 78,059,779 bp
  • C to T, chromosome 9 at 89,085,608 bp
  • G to A, chromosome 10 at 53,538,573 bp
  • T to C, chromosome 10 at 62,393,843 bp
  • A to G, chromosome 10 at 81,244,725 bp
  • G to A, chromosome 10 at 90,307,698 bp
  • A to T, chromosome 10 at 100,543,358 bp
  • A to T, chromosome 10 at 120,105,256 bp
  • T to C, chromosome 11 at 26,407,615 bp
  • T to A, chromosome 11 at 75,503,355 bp
  • T to A, chromosome 11 at 106,311,887 bp
  • C to T, chromosome 11 at 118,393,483 bp
  • C to T, chromosome 11 at 120,009,619 bp
  • G to T, chromosome 12 at 87,110,376 bp
  • A to T, chromosome 13 at 14,311,771 bp
  • A to G, chromosome 13 at 41,338,623 bp
  • C to T, chromosome 13 at 100,131,140 bp
  • C to A, chromosome 14 at 28,522,756 bp
  • T to C, chromosome 14 at 32,971,574 bp
  • C to A, chromosome 17 at 29,842,842 bp
  • T to A, chromosome 17 at 32,185,032 bp
  • T to A, chromosome 17 at 55,898,501 bp
  • C to G, chromosome 17 at 56,451,697 bp
  • T to A, chromosome 18 at 6,028,056 bp
  • C to T, chromosome 18 at 24,666,515 bp
  • T to C, chromosome 18 at 48,047,493 bp
  • A to G, chromosome 19 at 7,229,957 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7114 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045244-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.