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Strain Name:
C57BL/6J-MtgxR7165Btlr/Mmmh
Stock Number:
045262-MU
Citation ID:
RRID:MMRRC_045262-MU
Other Names:
R7165 (G1)
Major Collection:

Strain Information

Zmpste24
Name: zinc metallopeptidase, STE24
Synonyms: A530043O15Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230709
Homologene: 4277
Ap4e1
Name: adaptor-related protein complex AP-4, epsilon 1
Synonyms: 2310033A20Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 108011
HGNC: HGNC:573
Homologene: 22397
Ptx3
Name: pentraxin related gene
Synonyms: TSG-14, pentraxin 3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 19288
HGNC: HGNC:9692
Homologene: 7500
Frrs1
Name: ferric-chelate reductase 1
Synonyms: Sdfr2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 20321
Homologene: 40653
Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: Megalin, Gp330, D230004K18Rik, b2b1625.2Clo
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952
Plcd3
Name: phospholipase C, delta 3
Synonyms: 2610205J15Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 72469
HGNC: HGNC:9061
Homologene: 14858
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 4,349,917 bp
  • A to G, chromosome 1 at 33,628,393 bp
  • A to C, chromosome 1 at 36,155,107 bp
  • T to A, chromosome 1 at 39,292,717 bp
  • C to A, chromosome 1 at 100,076,162 bp
  • T to C, chromosome 1 at 136,149,448 bp
  • A to T, chromosome 1 at 151,388,677 bp
  • A to G, chromosome 1 at 156,828,248 bp
  • G to T, chromosome 1 at 162,673,517 bp
  • A to G, chromosome 1 at 181,704,535 bp
  • A to G, chromosome 2 at 52,270,306 bp
  • A to T, chromosome 2 at 69,506,573 bp
  • A to C, chromosome 2 at 76,827,914 bp
  • G to A, chromosome 2 at 82,981,197 bp
  • G to A, chromosome 2 at 117,338,404 bp
  • A to T, chromosome 2 at 120,704,158 bp
  • A to T, chromosome 2 at 127,063,318 bp
  • C to A, chromosome 2 at 128,123,108 bp
  • A to G, chromosome 2 at 144,393,998 bp
  • A to G, chromosome 2 at 168,182,367 bp
  • A to G, chromosome 3 at 66,224,970 bp
  • A to G, chromosome 3 at 108,016,377 bp
  • T to A, chromosome 3 at 116,878,271 bp
  • T to C, chromosome 4 at 42,938,325 bp
  • T to A, chromosome 4 at 45,308,549 bp
  • T to A, chromosome 4 at 65,261,873 bp
  • A to T, chromosome 4 at 121,082,894 bp
  • G to C, chromosome 4 at 139,450,513 bp
  • A to G, chromosome 4 at 144,372,819 bp
  • A to G, chromosome 4 at 151,084,700 bp
  • T to C, chromosome 4 at 156,252,290 bp
  • AACT to A, chromosome 5 at 25,950,851 bp
  • C to T, chromosome 5 at 30,375,620 bp
  • A to G, chromosome 5 at 73,491,195 bp
  • T to C, chromosome 5 at 90,772,589 bp
  • A to T, chromosome 5 at 113,745,995 bp
  • T to C, chromosome 5 at 142,972,046 bp
  • G to A, chromosome 6 at 28,446,137 bp
  • A to T, chromosome 6 at 92,191,334 bp
  • A to G, chromosome 6 at 120,350,232 bp
  • A to T, chromosome 6 at 146,294,091 bp
  • A to T, chromosome 7 at 23,702,651 bp
  • T to C, chromosome 7 at 24,978,965 bp
  • C to T, chromosome 7 at 38,099,301 bp
  • T to C, chromosome 7 at 118,555,934 bp
  • A to G, chromosome 7 at 140,005,361 bp
  • G to A, chromosome 9 at 7,050,479 bp
  • T to C, chromosome 9 at 27,334,240 bp
  • A to G, chromosome 9 at 31,316,442 bp
  • G to T, chromosome 9 at 37,664,085 bp
  • T to A, chromosome 9 at 39,023,270 bp
  • A to T, chromosome 9 at 50,917,097 bp
  • A to T, chromosome 9 at 54,709,232 bp
  • G to A, chromosome 9 at 113,786,399 bp
  • A to T, chromosome 9 at 120,019,047 bp
  • T to A, chromosome 10 at 117,773,362 bp
  • T to A, chromosome 11 at 31,029,029 bp
  • G to A, chromosome 11 at 78,590,967 bp
  • A to G, chromosome 11 at 97,033,808 bp
  • A to G, chromosome 11 at 103,079,613 bp
  • A to G, chromosome 12 at 79,280,405 bp
  • T to A, chromosome 12 at 111,321,517 bp
  • A to T, chromosome 13 at 13,367,620 bp
  • A to T, chromosome 13 at 30,224,415 bp
  • A to T, chromosome 13 at 56,628,016 bp
  • A to G, chromosome 13 at 59,586,183 bp
  • G to C, chromosome 13 at 73,514,530 bp
  • A to T, chromosome 13 at 99,414,799 bp
  • T to A, chromosome 14 at 32,175,983 bp
  • A to G, chromosome 15 at 76,690,904 bp
  • C to G, chromosome 16 at 32,282,361 bp
  • A to T, chromosome 16 at 37,535,866 bp
  • G to A, chromosome 16 at 55,232,231 bp
  • T to A, chromosome 17 at 28,333,254 bp
  • C to T, chromosome 17 at 30,909,323 bp
  • T to C, chromosome 17 at 32,338,412 bp
  • A to G, chromosome 17 at 35,621,978 bp
  • T to C, chromosome 18 at 7,002,525 bp
  • T to A, chromosome 18 at 37,505,070 bp
  • T to A, chromosome 18 at 37,763,178 bp
  • C to A, chromosome 18 at 84,015,927 bp
  • T to A, chromosome 19 at 30,585,199 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7165 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045262-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.