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Strain Name:
C57BL/6J-MtgxR7219Btlr/Mmmh
Stock Number:
045291-MU
Citation ID:
RRID:MMRRC_045291-MU
Other Names:
R7219 (G1)
Major Collection:

Strain Information

Myo5a
Name: myosin VA
Synonyms: MVa, MyoVA, Myo5, flail, 9630007J19Rik, Dbv
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17918
HGNC: HGNC:7602
Homologene: 20100
Cdc25a
Name: cell division cycle 25A
Synonyms: D9Ertd393e
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 12530
HGNC: HGNC:1725
Homologene: 1355
Pcdh9
Name: protocadherin 9
Synonyms: C630029H24Rik, 1500001L12Rik, LOC382930, A730003J17Rik, C530050I23Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 211712
HGNC: HGNC:8661
Homologene: 10698
Scn8a
Name: sodium channel, voltage-gated, type VIII, alpha
Synonyms: med, seal, motor end-plate disease, nmf2, ataxia 3, mnd2, mnd-2, C630029C19Rik, nmf58, NaCh6, Nav1.6, nmf335, NMF335, nur14
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 20273
Homologene: 7927
U2surp
Name: U2 snRNP-associated SURP domain containing
Synonyms: 2610101N10Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 67958
Homologene: 13964
Enox1
Name: ecto-NOX disulfide-thiol exchanger 1
Synonyms: D230005D02Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 239188
VEGA: 14
Homologene: 56793
Sbno1
Name: strawberry notch 1
Synonyms: sno, 9330180L10Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 243272
Homologene: 10055
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 1 at 54,766,910 bp
  • A to G, chromosome 1 at 60,502,873 bp
  • T to C, chromosome 1 at 93,560,318 bp
  • A to G, chromosome 1 at 130,462,606 bp
  • A to G, chromosome 1 at 174,222,637 bp
  • G to T, chromosome 1 at 192,505,637 bp
  • C to A, chromosome 2 at 71,530,169 bp
  • A to G, chromosome 2 at 91,492,023 bp
  • A to G, chromosome 2 at 103,028,415 bp
  • A to T, chromosome 2 at 111,369,537 bp
  • A to T, chromosome 2 at 111,500,187 bp
  • A to G, chromosome 2 at 120,503,454 bp
  • A to C, chromosome 2 at 173,022,197 bp
  • A to G, chromosome 3 at 60,603,823 bp
  • C to A, chromosome 3 at 92,081,398 bp
  • T to C, chromosome 4 at 15,266,700 bp
  • A to T, chromosome 4 at 150,935,534 bp
  • A to G, chromosome 5 at 30,657,452 bp
  • A to G, chromosome 5 at 88,649,856 bp
  • A to T, chromosome 5 at 120,630,135 bp
  • A to T, chromosome 5 at 120,802,892 bp
  • A to T, chromosome 5 at 124,405,659 bp
  • A to C, chromosome 6 at 28,243,663 bp
  • T to A, chromosome 6 at 41,311,703 bp
  • A to T, chromosome 6 at 122,629,959 bp
  • T to A, chromosome 6 at 133,107,932 bp
  • T to C, chromosome 7 at 4,528,077 bp
  • C to T, chromosome 7 at 7,387,106 bp
  • A to T, chromosome 7 at 7,394,398 bp
  • T to A, chromosome 7 at 64,204,585 bp
  • A to G, chromosome 7 at 102,781,706 bp
  • G to A, chromosome 8 at 13,079,907 bp
  • A to G, chromosome 8 at 14,743,296 bp
  • A to G, chromosome 8 at 33,546,240 bp
  • A to T, chromosome 8 at 45,350,563 bp
  • A to G, chromosome 8 at 47,858,884 bp
  • A to G, chromosome 8 at 69,706,706 bp
  • A to G, chromosome 8 at 91,001,766 bp
  • A to G, chromosome 9 at 27,005,210 bp
  • A to G, chromosome 9 at 75,120,770 bp
  • A to G, chromosome 9 at 89,984,288 bp
  • G to A, chromosome 9 at 95,490,162 bp
  • A to T, chromosome 9 at 108,081,286 bp
  • A to G, chromosome 9 at 109,889,086 bp
  • T to A, chromosome 10 at 80,584,174 bp
  • A to C, chromosome 10 at 127,557,228 bp
  • G to T, chromosome 10 at 128,634,383 bp
  • T to C, chromosome 11 at 21,323,271 bp
  • T to G, chromosome 11 at 55,168,918 bp
  • T to A, chromosome 11 at 97,711,197 bp
  • T to A, chromosome 11 at 115,877,095 bp
  • T to A, chromosome 12 at 83,957,870 bp
  • T to G, chromosome 12 at 113,326,596 bp
  • T to G, chromosome 12 at 118,041,095 bp
  • T to C, chromosome 12 at 118,126,889 bp
  • T to A, chromosome 13 at 3,590,521 bp
  • T to A, chromosome 13 at 94,224,907 bp
  • C to T, chromosome 14 at 26,854,880 bp
  • T to A, chromosome 14 at 77,720,844 bp
  • C to T, chromosome 14 at 93,015,780 bp
  • A to G, chromosome 15 at 58,054,337 bp
  • T to A, chromosome 15 at 100,969,103 bp
  • T to C, chromosome 16 at 4,849,644 bp
  • A to G, chromosome 16 at 10,512,257 bp
  • C to A, chromosome 16 at 49,908,077 bp
  • T to C, chromosome 16 at 88,884,909 bp
  • T to C, chromosome 16 at 91,665,001 bp
  • G to A, chromosome 17 at 24,185,292 bp
  • C to A, chromosome 17 at 29,319,234 bp
  • A to G, chromosome 17 at 30,982,262 bp
  • A to G, chromosome 17 at 34,679,065 bp
  • G to T, chromosome 17 at 46,935,434 bp
  • A to G, chromosome 17 at 47,702,695 bp
  • T to A, chromosome 17 at 56,998,508 bp
  • C to T, chromosome 18 at 58,053,027 bp
  • T to C, chromosome 18 at 76,860,401 bp
  • T to G, chromosome 18 at 78,755,745 bp
  • A to G, chromosome 19 at 3,286,998 bp
  • A to G, chromosome 19 at 4,724,173 bp
  • T to C, chromosome 19 at 47,791,473 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7219 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045291-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.