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Strain Name:
C57BL/6J-MtgxR7223Btlr/Mmmh
Stock Number:
045295-MU
Citation ID:
RRID:MMRRC_045295-MU
Other Names:
R7223 (G1)
Major Collection:

Strain Information

Sufu
Name: SUFU negative regulator of hedgehog signaling
Synonyms: Su(Fu), 2810026F04Rik, b2b273Clo
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 24069
Homologene: 9262
Htr1d
Name: 5-hydroxytryptamine (serotonin) receptor 1D
Synonyms: Gpcr14, Htr1db
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 15552
HGNC: HGNC:5289
Homologene: 20240
Jarid2
Name: jumonji and AT-rich interaction domain containing 2
Synonyms: Jmj, jumonji
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 16468
HGNC: HGNC:6196
Homologene: 31279
Tet1
Name: tet methylcytosine dioxygenase 1
Synonyms: 2510010B09Rik, D10Ertd17e, Cxxc6, BB001228
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 52463
Homologene: 12735
Pipox
Name: pipecolic acid oxidase
Synonyms: Pso
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 19193
Homologene: 40640
Tmem121
Name: transmembrane protein 121
Synonyms: Hole, 2410008J05Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 69195
Homologene: 11900
Dnajc24
Name: DnaJ heat shock protein family (Hsp40) member C24
Synonyms: MmDjC7, 2610027M02Rik, 1700030A21Rik, Zcsl3, Dph4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 99349
Homologene: 41704
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 43,968,888 bp
  • A to T, chromosome 1 at 80,287,000 bp
  • T to A, chromosome 1 at 146,901,074 bp
  • G to A, chromosome 1 at 150,439,256 bp
  • T to C, chromosome 1 at 171,407,571 bp
  • A to G, chromosome 1 at 188,810,217 bp
  • T to A, chromosome 2 at 62,268,665 bp
  • A to T, chromosome 2 at 69,274,143 bp
  • T to C, chromosome 2 at 76,890,981 bp
  • A to G, chromosome 2 at 83,878,230 bp
  • T to A, chromosome 2 at 86,455,867 bp
  • T to C, chromosome 2 at 104,043,012 bp
  • G to A, chromosome 2 at 106,001,966 bp
  • T to A, chromosome 2 at 132,534,056 bp
  • A to G, chromosome 2 at 143,690,333 bp
  • A to G, chromosome 2 at 163,072,705 bp
  • A to T, chromosome 3 at 20,030,419 bp
  • T to C, chromosome 4 at 70,235,447 bp
  • C to T, chromosome 4 at 126,275,152 bp
  • T to A, chromosome 4 at 136,443,501 bp
  • A to T, chromosome 5 at 106,637,582 bp
  • G to T, chromosome 5 at 129,900,438 bp
  • A to T, chromosome 5 at 130,190,960 bp
  • A to T, chromosome 6 at 30,812,754 bp
  • C to T, chromosome 7 at 11,846,967 bp
  • G to A, chromosome 7 at 24,624,653 bp
  • A to T, chromosome 7 at 26,814,632 bp
  • A to T, chromosome 7 at 66,783,415 bp
  • A to T, chromosome 7 at 79,825,310 bp
  • G to T, chromosome 7 at 99,268,025 bp
  • C to A, chromosome 7 at 103,041,632 bp
  • A to G, chromosome 7 at 119,451,498 bp
  • A to T, chromosome 8 at 43,652,004 bp
  • A to T, chromosome 8 at 70,868,526 bp
  • T to C, chromosome 9 at 38,143,753 bp
  • T to A, chromosome 9 at 73,629,191 bp
  • C to T, chromosome 9 at 86,879,557 bp
  • T to C, chromosome 9 at 103,499,534 bp
  • T to A, chromosome 9 at 120,094,617 bp
  • G to A, chromosome 9 at 121,915,097 bp
  • T to C, chromosome 10 at 3,873,343 bp
  • T to A, chromosome 10 at 60,331,817 bp
  • T to C, chromosome 10 at 62,813,671 bp
  • C to T, chromosome 10 at 78,583,696 bp
  • G to A, chromosome 10 at 79,960,368 bp
  • G to T, chromosome 10 at 128,112,606 bp
  • C to A, chromosome 10 at 128,199,194 bp
  • A to G, chromosome 11 at 22,973,388 bp
  • C to T, chromosome 11 at 30,874,226 bp
  • T to A, chromosome 11 at 49,564,098 bp
  • T to A, chromosome 11 at 57,530,240 bp
  • A to G, chromosome 11 at 77,881,186 bp
  • T to C, chromosome 12 at 71,988,943 bp
  • C to T, chromosome 12 at 72,074,009 bp
  • T to C, chromosome 12 at 79,246,171 bp
  • A to T, chromosome 12 at 113,188,494 bp
  • T to C, chromosome 13 at 9,710,162 bp
  • T to A, chromosome 13 at 21,317,670 bp
  • A to G, chromosome 13 at 44,896,322 bp
  • A to G, chromosome 13 at 47,014,208 bp
  • A to T, chromosome 13 at 95,628,972 bp
  • T to C, chromosome 14 at 55,496,238 bp
  • T to C, chromosome 14 at 122,476,091 bp
  • A to T, chromosome 15 at 9,601,640 bp
  • G to T, chromosome 15 at 39,437,032 bp
  • T to C, chromosome 15 at 97,010,345 bp
  • T to C, chromosome 15 at 99,279,837 bp
  • T to A, chromosome 16 at 32,104,015 bp
  • A to G, chromosome 16 at 87,362,660 bp
  • T to C, chromosome 17 at 8,256,140 bp
  • G to A, chromosome 17 at 23,609,482 bp
  • T to C, chromosome 17 at 24,881,234 bp
  • G to A, chromosome 17 at 25,243,840 bp
  • T to C, chromosome 17 at 26,839,620 bp
  • A to G, chromosome 17 at 40,731,081 bp
  • C to T, chromosome 18 at 12,582,608 bp
  • T to G, chromosome 18 at 36,993,148 bp
  • T to C, chromosome 18 at 43,438,042 bp
  • T to C, chromosome 18 at 67,366,421 bp
  • A to C, chromosome 19 at 3,979,592 bp
  • G to A, chromosome 19 at 6,388,863 bp
  • T to C, chromosome 19 at 46,453,277 bp
  • C to T, chromosome 19 at 50,190,042 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7223 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045295-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.