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Strain Name:
C57BL/6J-MtgxR7225Btlr/Mmmh
Stock Number:
045297-MU
Citation ID:
RRID:MMRRC_045297-MU
Other Names:
R7225 (G1)
Major Collection:

Strain Information

Ptprz1
Name: protein tyrosine phosphatase receptor type Z, polypeptide 1
Synonyms: DSD-1-PG, phosphacan, PTPzeta, PTPbeta, Rptpbeta, Ptpz, Ptprz, RPTPz
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 19283
HGNC: HGNC:9685
Homologene: 2136
Gria2
Name: glutamate receptor, ionotropic, AMPA2 (alpha 2)
Synonyms: GluR-B, GluR2, Glur-2, Glur2, GluA2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14800
HGNC: HGNC:4572
Homologene: 20225
Tnfsf4
Name: tumor necrosis factor (ligand) superfamily, member 4
Synonyms: OX40L, gp34, Txgp1l, Ath-1, TXGP1, CD134L, Ath1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 22164
Homologene: 2495
Plin3
Name: perilipin 3
Synonyms: 1300012C15Rik, Tip47, M6prbp1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 66905
VEGA: 17
Homologene: 4247
Jmjd1c
Name: jumonji domain containing 1C
Synonyms: TRIP8, 5430433L24Rik, D630035I23Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 108829
Homologene: 3129
Oxr1
Name: oxidation resistance 1
Synonyms: C7B, C7, 2210416C20Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 170719
VEGA: 15
Homologene: 24993
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 34,472,846 bp
  • T to C, chromosome 1 at 36,420,952 bp
  • A to T, chromosome 1 at 133,387,124 bp
  • A to G, chromosome 1 at 161,417,250 bp
  • T to C, chromosome 2 at 25,565,546 bp
  • C to T, chromosome 2 at 32,961,897 bp
  • T to A, chromosome 2 at 70,684,047 bp
  • T to C, chromosome 2 at 120,724,912 bp
  • T to G, chromosome 2 at 128,801,562 bp
  • T to C, chromosome 2 at 150,409,005 bp
  • T to C, chromosome 3 at 38,980,176 bp
  • T to A, chromosome 3 at 80,802,631 bp
  • A to C, chromosome 4 at 80,911,481 bp
  • A to G, chromosome 4 at 120,746,914 bp
  • A to G, chromosome 4 at 155,949,316 bp
  • T to A, chromosome 5 at 48,380,233 bp
  • C to T, chromosome 5 at 72,700,714 bp
  • C to A, chromosome 5 at 104,065,100 bp
  • T to C, chromosome 5 at 109,677,149 bp
  • A to G, chromosome 5 at 135,732,587 bp
  • G to A, chromosome 6 at 23,000,929 bp
  • G to A, chromosome 6 at 42,293,462 bp
  • T to A, chromosome 6 at 97,247,384 bp
  • A to T, chromosome 7 at 28,898,699 bp
  • A to C, chromosome 7 at 29,399,428 bp
  • C to T, chromosome 7 at 30,877,634 bp
  • A to G, chromosome 7 at 36,769,657 bp
  • A to G, chromosome 7 at 49,770,856 bp
  • AGTGT to AGT, chromosome 7 at 55,928,189 bp
  • T to A, chromosome 7 at 101,711,465 bp
  • A to G, chromosome 7 at 133,853,259 bp
  • T to A, chromosome 7 at 144,285,025 bp
  • C to A, chromosome 8 at 71,685,511 bp
  • A to G, chromosome 9 at 7,142,756 bp
  • T to G, chromosome 9 at 120,131,156 bp
  • T to C, chromosome 10 at 67,226,065 bp
  • A to G, chromosome 10 at 81,622,481 bp
  • A to G, chromosome 10 at 92,904,374 bp
  • C to T, chromosome 10 at 127,191,489 bp
  • G to A, chromosome 10 at 130,386,683 bp
  • T to G, chromosome 11 at 74,666,406 bp
  • T to C, chromosome 11 at 94,097,358 bp
  • C to T, chromosome 11 at 100,580,587 bp
  • A to G, chromosome 12 at 17,175,693 bp
  • T to C, chromosome 12 at 75,468,579 bp
  • T to C, chromosome 12 at 110,670,865 bp
  • A to G, chromosome 12 at 111,423,624 bp
  • A to G, chromosome 13 at 73,763,962 bp
  • T to C, chromosome 14 at 104,479,858 bp
  • T to G, chromosome 14 at 115,552,298 bp
  • C to T, chromosome 15 at 16,856,073 bp
  • C to T, chromosome 15 at 41,813,608 bp
  • G to T, chromosome 15 at 44,546,941 bp
  • G to A, chromosome 15 at 64,130,250 bp
  • A to G, chromosome 16 at 29,614,039 bp
  • T to C, chromosome 16 at 91,027,068 bp
  • T to C, chromosome 17 at 31,854,300 bp
  • T to C, chromosome 17 at 56,286,541 bp
  • T to C, chromosome 18 at 15,452,661 bp
  • C to T, chromosome 18 at 37,444,437 bp
  • T to C, chromosome 18 at 60,828,448 bp
  • T to C, chromosome 18 at 65,305,199 bp
  • T to A, chromosome 18 at 78,012,702 bp
  • A to T, chromosome 19 at 12,283,467 bp
  • G to T, chromosome 19 at 47,267,389 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7225 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045297-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.