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Strain Name:
C57BL/6J-MtgxR7226Btlr/Mmmh
Stock Number:
045298-MU
Citation ID:
RRID:MMRRC_045298-MU
Other Names:
R7226 (G1)
Major Collection:

Strain Information

Atrn
Name: attractin
Synonyms: Mgca
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 11990
HGNC: HGNC:885
Homologene: 22542
Ehmt1
Name: euchromatic histone methyltransferase 1
Synonyms: 9230102N17Rik, KMT1D
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 77683
Homologene: 11698
Rad54l2
Name: RAD54 like 2 (S. cerevisiae)
Synonyms: Arip4, G630026H09Rik, Srisnf2l
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 81000
Homologene: 56698
Mov10
Name: Mov10 RISC complex RNA helicase
Synonyms: Mov-10
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 17454
HGNC: HGNC:7200
Homologene: 10365
Ap2m1
Name: adaptor-related protein complex 2, mu 1 subunit
Synonyms: clathrin-associated AP-2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 11773
HGNC: HGNC:564
Homologene: 3000
Dhx9
Name: DExH-box helicase 9
Synonyms: leukophysin, nuclear DNA helicase II, RNA helicase, Ddx9, NDH II, NDHII, RHA
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13211
HGNC: HGNC:2750
Homologene: 1039
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 74,737,348 bp
  • A to T, chromosome 1 at 132,302,606 bp
  • A to T, chromosome 1 at 153,465,677 bp
  • A to G, chromosome 1 at 195,042,006 bp
  • T to C, chromosome 2 at 24,804,782 bp
  • T to C, chromosome 2 at 25,224,168 bp
  • T to C, chromosome 2 at 103,726,753 bp
  • T to A, chromosome 2 at 104,050,297 bp
  • C to A, chromosome 2 at 122,522,532 bp
  • T to G, chromosome 2 at 130,986,744 bp
  • C to A, chromosome 2 at 178,421,203 bp
  • A to G, chromosome 3 at 97,050,858 bp
  • G to T, chromosome 3 at 104,801,012 bp
  • T to A, chromosome 4 at 14,892,464 bp
  • C to A, chromosome 4 at 101,639,372 bp
  • T to A, chromosome 4 at 125,071,480 bp
  • C to A, chromosome 4 at 130,771,981 bp
  • C to T, chromosome 4 at 134,006,308 bp
  • C to A, chromosome 5 at 75,075,960 bp
  • T to C, chromosome 5 at 112,337,456 bp
  • T to C, chromosome 5 at 120,460,637 bp
  • T to A, chromosome 5 at 122,921,449 bp
  • T to A, chromosome 5 at 134,901,684 bp
  • T to A, chromosome 5 at 134,935,803 bp
  • T to A, chromosome 5 at 147,526,992 bp
  • A to T, chromosome 7 at 16,198,876 bp
  • A to T, chromosome 7 at 26,558,724 bp
  • A to T, chromosome 7 at 42,613,260 bp
  • A to T, chromosome 7 at 108,158,957 bp
  • A to G, chromosome 7 at 126,414,823 bp
  • A to T, chromosome 8 at 24,647,808 bp
  • T to A, chromosome 8 at 84,864,060 bp
  • T to A, chromosome 8 at 95,637,871 bp
  • T to A, chromosome 9 at 26,978,430 bp
  • C to T, chromosome 9 at 31,100,152 bp
  • C to A, chromosome 9 at 106,713,472 bp
  • T to C, chromosome 9 at 121,746,533 bp
  • A to G, chromosome 10 at 81,468,029 bp
  • A to T, chromosome 10 at 89,808,641 bp
  • A to C, chromosome 10 at 117,361,822 bp
  • A to T, chromosome 10 at 128,625,218 bp
  • G to T, chromosome 10 at 128,940,932 bp
  • G to A, chromosome 10 at 129,570,715 bp
  • G to T, chromosome 11 at 69,369,211 bp
  • A to G, chromosome 11 at 73,776,677 bp
  • A to T, chromosome 11 at 80,971,514 bp
  • A to C, chromosome 11 at 100,970,458 bp
  • A to G, chromosome 12 at 112,737,925 bp
  • A to G, chromosome 13 at 25,253,468 bp
  • G to A, chromosome 13 at 36,730,603 bp
  • A to T, chromosome 13 at 74,406,995 bp
  • A to G, chromosome 14 at 20,460,195 bp
  • A to G, chromosome 14 at 51,365,351 bp
  • T to C, chromosome 14 at 57,035,893 bp
  • C to T, chromosome 15 at 58,590,535 bp
  • C to T, chromosome 15 at 77,558,989 bp
  • T to A, chromosome 16 at 20,539,451 bp
  • T to C, chromosome 16 at 44,220,005 bp
  • T to A, chromosome 16 at 56,267,104 bp
  • A to T, chromosome 17 at 18,451,983 bp
  • A to G, chromosome 17 at 35,142,945 bp
  • T to A, chromosome 17 at 35,397,113 bp
  • C to T, chromosome 17 at 35,691,147 bp
  • C to T, chromosome 17 at 38,130,433 bp
  • T to C, chromosome 17 at 45,559,227 bp
  • A to G, chromosome 17 at 74,202,343 bp
  • A to T, chromosome 18 at 24,584,027 bp
  • A to G, chromosome 18 at 58,037,070 bp
  • A to T, chromosome 18 at 66,083,128 bp
  • A to G, chromosome 18 at 87,757,466 bp
  • T to A, chromosome 19 at 11,978,667 bp
  • T to C, chromosome 19 at 40,791,307 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7226 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045298-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.