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Strain Name:
C57BL/6J-MtgxR7253Btlr/Mmmh
Stock Number:
045314-MU
Citation ID:
RRID:MMRRC_045314-MU
Other Names:
R7253 (G1)
Major Collection:

Strain Information

Pank4
Name: pantothenate kinase 4
Synonyms: D030031I12Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 269614
Homologene: 41235
Smarca4
Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Synonyms: SNF2beta, Brg1, SW1/SNF, b2b692Clo, b2b508.1Clo
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20586
Homologene: 135927
Ptx3
Name: pentraxin related gene
Synonyms: TSG-14, pentraxin 3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 19288
HGNC: HGNC:9692
Homologene: 7500
Pias2
Name: protein inhibitor of activated STAT 2
Synonyms: Dib, 6330408K17Rik, ARIP3, PIASxbeta, PIASxalpha, PIASxb, Miz1
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 17344
VEGA: 18
Homologene: 20979
Map3k4
Name: mitogen-activated protein kinase kinase kinase 4
Synonyms: MTK1, MAPKKK4, D17Rp17, RP17, D17Rp17e, Mekk4, T-associated sex reversal, Tas
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 26407
VEGA: 17
HGNC: HGNC:6856
Homologene: 31346
E2f7
Name: E2F transcription factor 7
Synonyms: A630014C11Rik, D10Ertd739e, E2F7
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 52679
Homologene: 18685
Hspbap1
Name: Hspb associated protein 1
Synonyms: 3830421G21Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 66667
VEGA: 16
Homologene: 11617
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 57,961,956 bp
  • C to A, chromosome 2 at 19,658,931 bp
  • A to G, chromosome 2 at 29,699,721 bp
  • A to C, chromosome 2 at 67,513,482 bp
  • A to G, chromosome 2 at 85,399,639 bp
  • T to C, chromosome 2 at 88,683,625 bp
  • G to T, chromosome 2 at 102,346,968 bp
  • G to T, chromosome 2 at 158,547,656 bp
  • G to T, chromosome 2 at 164,355,547 bp
  • G to T, chromosome 3 at 66,224,947 bp
  • C to T, chromosome 3 at 86,793,259 bp
  • A to G, chromosome 4 at 39,451,391 bp
  • A to T, chromosome 4 at 60,582,078 bp
  • A to T, chromosome 4 at 85,118,473 bp
  • A to T, chromosome 4 at 101,835,528 bp
  • G to A, chromosome 4 at 103,139,347 bp
  • A to C, chromosome 4 at 137,519,946 bp
  • A to G, chromosome 4 at 154,970,920 bp
  • A to G, chromosome 5 at 20,765,858 bp
  • G to A, chromosome 5 at 103,565,284 bp
  • G to A, chromosome 5 at 105,481,656 bp
  • A to G, chromosome 5 at 121,314,881 bp
  • A to G, chromosome 5 at 121,778,021 bp
  • T to C, chromosome 6 at 43,167,810 bp
  • C to A, chromosome 6 at 52,777,652 bp
  • T to A, chromosome 6 at 86,465,808 bp
  • T to A, chromosome 6 at 125,106,592 bp
  • C to T, chromosome 7 at 19,094,976 bp
  • C to T, chromosome 7 at 23,417,391 bp
  • A to G, chromosome 7 at 42,661,612 bp
  • T to C, chromosome 7 at 44,014,789 bp
  • T to C, chromosome 7 at 78,781,459 bp
  • GGCGGCGGC to GGCGGCGGCCGCGGCGGC, chromosome 7 at 97,579,915 bp
  • A to G, chromosome 7 at 102,112,086 bp
  • C to T, chromosome 7 at 103,538,788 bp
  • A to T, chromosome 7 at 141,107,437 bp
  • C to A, chromosome 8 at 70,789,682 bp
  • C to T, chromosome 8 at 95,095,497 bp
  • T to G, chromosome 9 at 5,324,868 bp
  • T to C, chromosome 9 at 21,658,960 bp
  • T to C, chromosome 9 at 22,002,471 bp
  • T to A, chromosome 9 at 44,264,704 bp
  • T to C, chromosome 9 at 59,737,195 bp
  • A to G, chromosome 9 at 71,215,934 bp
  • C to A, chromosome 9 at 85,326,717 bp
  • G to T, chromosome 9 at 101,031,913 bp
  • T to C, chromosome 9 at 106,155,462 bp
  • A to G, chromosome 10 at 17,827,885 bp
  • T to G, chromosome 10 at 39,155,035 bp
  • A to G, chromosome 10 at 41,432,484 bp
  • G to T, chromosome 10 at 53,698,805 bp
  • C to A, chromosome 10 at 56,198,441 bp
  • T to A, chromosome 10 at 107,608,273 bp
  • A to G, chromosome 10 at 110,766,303 bp
  • C to T, chromosome 10 at 127,481,775 bp
  • T to C, chromosome 11 at 58,778,996 bp
  • T to A, chromosome 11 at 58,817,540 bp
  • A to T, chromosome 11 at 59,971,255 bp
  • T to C, chromosome 11 at 70,345,898 bp
  • T to C, chromosome 11 at 86,143,278 bp
  • C to A, chromosome 11 at 106,047,843 bp
  • T to C, chromosome 12 at 80,958,184 bp
  • T to C, chromosome 12 at 108,351,996 bp
  • C to T, chromosome 12 at 113,655,108 bp
  • C to A, chromosome 13 at 4,577,140 bp
  • T to C, chromosome 15 at 97,245,075 bp
  • T to C, chromosome 15 at 101,450,511 bp
  • G to T, chromosome 16 at 35,817,230 bp
  • T to A, chromosome 17 at 12,272,068 bp
  • T to C, chromosome 17 at 33,934,990 bp
  • G to A, chromosome 17 at 43,037,667 bp
  • G to A, chromosome 18 at 9,848,922 bp
  • T to G, chromosome 18 at 77,120,115 bp
  • A to T, chromosome 19 at 10,262,581 bp
  • A to G, chromosome 19 at 10,916,569 bp
  • A to G, chromosome 19 at 11,520,396 bp
  • A to G, chromosome 19 at 38,698,508 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7253 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045314-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.