Strain Name:
C57BL/6J-MtgxR6546Btlr/Mmmh
Stock Number:
045325-MU
Citation ID:
RRID:MMRRC_045325-MU
Other Names:
R6546 (G1)
Major Collection:

Strain Information

Chrna3
Name: cholinergic receptor, nicotinic, alpha polypeptide 3
Synonyms: (a)3, alpha 3, neuronal nicotinic acetylcholine receptor, alpha 3 subunit, Acra-3, Acra3, A730007P14Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 110834
VEGA: 9
HGNC: HGNC:1957
Homologene: 591
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Cdk18
Name: cyclin dependent kinase 18
Synonyms: Pctk3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18557
HGNC: HGNC:8751
Homologene: 1949
Med13l
Name: mediator complex subunit 13-like
Synonyms: 2210413I17Rik, 6330591G05Rik, Trap240L, 9030618F05Rik, Thrap2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 76199
Homologene: 25256
Syne1
Name: spectrin repeat containing, nuclear envelope 1
Synonyms: nesprin-1, SYNE-1, enaptin165, A330049M09Rik, C130039F11Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 64009
VEGA: 10
Homologene: 52329
St7
Name: suppression of tumorigenicity 7
Synonyms: RAY1, HELG, SEN4, TSG7, Fam4a2, 9430001H04Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 64213
Homologene: 10185
Trmt6
Name: tRNA methyltransferase 6
Synonyms: 3300001M20Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 66926
Homologene: 6816
Ppil4
Name: peptidylprolyl isomerase (cyclophilin)-like 4
Synonyms: PPIase, 3732410E19Rik, 3830425H19Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 67418
Homologene: 12126
Ubr4
Name: ubiquitin protein ligase E3 component n-recognin 4
Synonyms: A930005E13Rik, LOC381562, D930005K06Rik, 1810009A16Rik, Zubr1, p600
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 69116
Homologene: 10804
Krt10
Name: keratin 10
Synonyms: K10, K1C1, suprabasal cytokeratin 10, Krt-1.10, keratin 10, cytokeratin 10, D130054E02Rik, Krt1-10
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16661
HGNC: HGNC:6413
Homologene: 137236
Rbl2
Name: RB transcriptional corepressor like 2
Synonyms: Rb2, p130, retinoblastoma-like 2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 19651
HGNC: HGNC:9894
Homologene: 4098
Jmjd6
Name: jumonji domain containing 6
Synonyms: PtdSerR, 5730436I23Rik, PSR, Ptdsr
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 107817
Homologene: 9046
Nup58
Name: nucleoporin 58
Synonyms: 1700017F11Rik, Nupl1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 71844
VEGA: 14
Homologene: 40924
Fnip1
Name: folliculin interacting protein 1
Synonyms: A730024A03Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216742
Homologene: 28173
Atad2b
Name: ATPase family, AAA domain containing 2B
Synonyms: 1110014E10Rik, D530031C13Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 320817
VEGA: 12
Homologene: 86351
Zp2
Name: zona pellucida glycoprotein 2
Synonyms: Zp-2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22787
Homologene: 48194
Syn2
Name: synapsin II
Synonyms: 2900074L19Rik, Synapsin IIb, Synapsin IIa
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 20965
Homologene: 49348
Dnah14
Name: dynein, axonemal, heavy chain 14
Synonyms: LOC381311, A230079K17Rik, Gm980, Dnahc14
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 240960
HGNC: HGNC:2945
Homologene: 90078
Ulk4
Name: unc-51-like kinase 4
Synonyms: 4932415A06Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 209012
Homologene: 41205
Agbl3
Name: ATP/GTP binding protein-like 3
Synonyms: 2900053G10Rik, 6530406M24Rik, 4930431N21Rik, Ccp3, Ccp3
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 76223
Homologene: 35330
Vmn2r111
Name: vomeronasal 2, receptor 111
Synonyms: EG210876
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 210876
Homologene: 86604
Map3k13
Name: mitogen-activated protein kinase kinase kinase 13
Synonyms: C130026N12Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 71751
VEGA: 16
HGNC: HGNC:6852
Homologene: 37958
Papss2
Name: 3'-phosphoadenosine 5'-phosphosulfate synthase 2
Synonyms: Sk2, Atpsk2, 1810018P12Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 23972
VEGA: 19
HGNC: HGNC:8604
Homologene: 55840
Vmn2r79
Name: vomeronasal 2, receptor 79
Synonyms: EG621430
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 621430
Homologene: 115466
Mat1a
Name: methionine adenosyltransferase 1A
Synonyms: AdoMet, SAMS, MAT, SAMS1, MATA1, Ams
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 11720
VEGA: 14
HGNC: HGNC:6903
Homologene: 68057
Dhrs1
Name: dehydrogenase/reductase 1
Synonyms: 1110029G07Rik, dehydrogenase/reductase (SDR family) member 1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 52585
Homologene: 41696
Vmn2r27
Name: vomeronasal 2, receptor27
Synonyms: EG232367
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232367
Rchy1
Name: ring finger and CHY zinc finger domain containing 1
Synonyms: PRO1996, 6720407C15Rik, Zfp363, Pirh2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 68098
Homologene: 22894
Kif26b
Name: kinesin family member 26B
Synonyms: N-11 kinesin, D230039L06Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 269152
Homologene: 18623
Garin5b
Name: golgi associated RAB2 interactor family member 5B
Synonyms: 4930401F20Rik, Fam71e2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 243822
Homologene: 89225
Bmp2k
Name: BMP2 inducible kinase
Synonyms: BIKE, 4933417M22Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 140780
Homologene: 34134
Npffr2
Name: neuropeptide FF receptor 2
Synonyms: NPFF2, Gpr74
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 104443
HGNC: HGNC:4525
Homologene: 56982
Qrfpr
Name: pyroglutamylated RFamide peptide receptor
Synonyms: AQ27, Gpr103
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229214
Homologene: 18865
Slc19a3
Name: solute carrier family 19, member 3
Synonyms: ThTr2, A230084E24Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 80721
Homologene: 23530
Kbtbd4
Name: kelch repeat and BTB (POZ) domain containing 4
Synonyms: 2510026C23Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 67136
Homologene: 32320
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 83,026,360 bp
  • G to A, chromosome 1 at 132,122,350 bp
  • T to C, chromosome 1 at 178,928,306 bp
  • C to T, chromosome 1 at 181,738,987 bp
  • T to A, chromosome 2 at 90,909,291 bp
  • A to G, chromosome 2 at 132,812,153 bp
  • G to A, chromosome 3 at 36,180,265 bp
  • T to C, chromosome 4 at 123,432,281 bp
  • G to A, chromosome 4 at 139,414,394 bp
  • A to T, chromosome 5 at 89,583,012 bp
  • G to A, chromosome 5 at 91,957,958 bp
  • A to G, chromosome 5 at 97,088,078 bp
  • T to C, chromosome 5 at 118,721,474 bp
  • C to T, chromosome 6 at 17,852,314 bp
  • T to A, chromosome 6 at 34,799,299 bp
  • T to A, chromosome 6 at 115,281,098 bp
  • T to A, chromosome 6 at 124,192,410 bp
  • T to C, chromosome 7 at 4,758,465 bp
  • A to G, chromosome 7 at 87,003,533 bp
  • T to C, chromosome 7 at 120,132,525 bp
  • C to A, chromosome 8 at 91,070,370 bp
  • T to A, chromosome 9 at 55,015,901 bp
  • A to T, chromosome 9 at 121,141,894 bp
  • A to T, chromosome 10 at 5,218,645 bp
  • T to A, chromosome 10 at 7,798,422 bp
  • T to G, chromosome 11 at 54,502,611 bp
  • T to C, chromosome 11 at 99,387,395 bp
  • T to C, chromosome 11 at 116,842,500 bp
  • A to T, chromosome 12 at 4,990,949 bp
  • G to A, chromosome 14 at 41,121,422 bp
  • G to A, chromosome 14 at 55,741,272 bp
  • A to T, chromosome 14 at 60,223,223 bp
  • C to T, chromosome 16 at 21,921,777 bp
  • T to C, chromosome 17 at 22,559,051 bp
  • A to G, chromosome 19 at 32,663,148 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6546 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045325-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.