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Strain Name:
C57BL/6J-MtgxR7144Btlr/Mmmh
Stock Number:
045328-MU
Citation ID:
RRID:MMRRC_045328-MU
Other Names:
R7144 (G1)
Major Collection:

Strain Information

St8sia1
Name: ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1
Synonyms: alpha-2,8-sialyltransferase, ST8Sia I, GD3S, GD3 synthase, Siat8, Siat8a, 9330109E03Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 20449
Homologene: 2282
Myo10
Name: myosin X
Synonyms: myosin-X, D15Ertd600e
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 17909
HGNC: HGNC:7593
Homologene: 36328
Ap4e1
Name: adaptor-related protein complex AP-4, epsilon 1
Synonyms: 2310033A20Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 108011
HGNC: HGNC:573
Homologene: 22397
Limch1
Name: LIM and calponin homology domains 1
Synonyms: 3732412D22Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 77569
Homologene: 18953
Adcy10
Name: adenylate cyclase 10
Synonyms: soluble adenylyl cyclase, sAC, 4930431D04Rik, Sacy
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 271639
Homologene: 10188
Dst
Name: dystonin
Synonyms: bullous pemphigoid antigen 1, BPAG1-n, BPAG1, Bpag1, Bpag, ah, bullous pemphigoid antigen 1, athetoid, Macf2, 2310001O04Rik, nmf203, A830042E19Rik, nmf339
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Cdk2ap1
Name: cyclin dependent kinase 2 associated protein 1
Synonyms: ST19, DORC1, Doc1, p12
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 13445
Homologene: 3411
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 1 at 6,833,594 bp
  • A to G, chromosome 1 at 34,152,243 bp
  • A to T, chromosome 1 at 53,698,708 bp
  • G to T, chromosome 1 at 58,753,848 bp
  • G to C, chromosome 1 at 60,237,151 bp
  • A to T, chromosome 1 at 84,793,714 bp
  • A to C, chromosome 1 at 92,982,631 bp
  • T to C, chromosome 1 at 150,663,873 bp
  • A to T, chromosome 1 at 155,242,031 bp
  • A to G, chromosome 1 at 158,295,424 bp
  • G to T, chromosome 1 at 165,510,370 bp
  • A to G, chromosome 1 at 166,677,620 bp
  • C to T, chromosome 1 at 167,308,453 bp
  • A to G, chromosome 2 at 6,206,413 bp
  • T to C, chromosome 2 at 20,865,387 bp
  • T to G, chromosome 2 at 29,943,793 bp
  • A to G, chromosome 2 at 32,419,166 bp
  • T to A, chromosome 2 at 45,110,041 bp
  • G to T, chromosome 2 at 86,988,820 bp
  • T to C, chromosome 2 at 89,527,557 bp
  • T to C, chromosome 2 at 91,134,604 bp
  • T to A, chromosome 2 at 127,011,807 bp
  • T to G, chromosome 2 at 146,950,510 bp
  • A to G, chromosome 2 at 178,302,736 bp
  • G to T, chromosome 3 at 55,045,699 bp
  • A to G, chromosome 3 at 64,089,941 bp
  • T to A, chromosome 3 at 88,116,910 bp
  • G to T, chromosome 3 at 100,027,401 bp
  • T to C, chromosome 3 at 153,411,532 bp
  • T to C, chromosome 4 at 116,975,976 bp
  • A to T, chromosome 4 at 126,043,722 bp
  • A to G, chromosome 4 at 138,605,431 bp
  • A to C, chromosome 4 at 143,991,533 bp
  • T to G, chromosome 4 at 155,589,336 bp
  • A to G, chromosome 5 at 34,561,631 bp
  • T to C, chromosome 5 at 34,846,006 bp
  • A to G, chromosome 5 at 37,386,839 bp
  • A to T, chromosome 5 at 45,496,948 bp
  • A to G, chromosome 5 at 67,017,658 bp
  • A to G, chromosome 5 at 93,156,866 bp
  • G to A, chromosome 5 at 124,354,358 bp
  • A to G, chromosome 5 at 124,822,942 bp
  • C to A, chromosome 5 at 137,071,064 bp
  • A to T, chromosome 6 at 121,761,587 bp
  • A to G, chromosome 6 at 123,181,384 bp
  • G to A, chromosome 6 at 125,176,670 bp
  • T to G, chromosome 6 at 135,733,476 bp
  • C to A, chromosome 6 at 139,629,870 bp
  • T to C, chromosome 6 at 142,876,669 bp
  • T to C, chromosome 6 at 145,961,155 bp
  • A to G, chromosome 7 at 3,897,616 bp
  • A to G, chromosome 7 at 4,472,185 bp
  • T to C, chromosome 7 at 29,935,165 bp
  • T to A, chromosome 7 at 83,646,451 bp
  • T to A, chromosome 7 at 106,873,868 bp
  • A to G, chromosome 7 at 119,805,191 bp
  • A to T, chromosome 7 at 120,433,573 bp
  • G to T, chromosome 7 at 126,796,862 bp
  • T to C, chromosome 7 at 133,637,849 bp
  • T to C, chromosome 7 at 143,811,215 bp
  • T to G, chromosome 8 at 4,135,189 bp
  • T to A, chromosome 8 at 64,124,945 bp
  • A to T, chromosome 8 at 117,076,131 bp
  • A to T, chromosome 8 at 124,374,317 bp
  • A to T, chromosome 9 at 7,475,318 bp
  • A to T, chromosome 9 at 60,615,156 bp
  • T to C, chromosome 9 at 79,820,213 bp
  • T to C, chromosome 9 at 85,743,691 bp
  • A to C, chromosome 9 at 98,601,471 bp
  • T to C, chromosome 9 at 101,964,077 bp
  • G to A, chromosome 9 at 105,650,584 bp
  • T to A, chromosome 9 at 123,431,993 bp
  • T to A, chromosome 10 at 19,007,281 bp
  • A to C, chromosome 10 at 23,173,045 bp
  • T to C, chromosome 10 at 80,637,899 bp
  • A to T, chromosome 10 at 83,573,774 bp
  • A to C, chromosome 11 at 54,657,365 bp
  • G to C, chromosome 11 at 58,141,663 bp
  • A to C, chromosome 11 at 58,732,745 bp
  • A to C, chromosome 11 at 65,218,648 bp
  • A to T, chromosome 11 at 99,416,013 bp
  • C to A, chromosome 12 at 76,005,378 bp
  • T to C, chromosome 12 at 91,294,159 bp
  • A to G, chromosome 12 at 103,142,626 bp
  • T to G, chromosome 12 at 103,947,018 bp
  • A to G, chromosome 13 at 113,083,371 bp
  • T to C, chromosome 14 at 53,743,639 bp
  • G to A, chromosome 14 at 56,512,332 bp
  • A to T, chromosome 15 at 25,723,925 bp
  • T to A, chromosome 15 at 66,913,030 bp
  • T to A, chromosome 15 at 68,178,782 bp
  • A to G, chromosome 15 at 91,734,055 bp
  • A to G, chromosome 17 at 32,357,628 bp
  • A to G, chromosome 17 at 58,286,888 bp
  • T to A, chromosome 18 at 37,443,256 bp
  • A to T, chromosome 19 at 39,615,694 bp
  • G to A, chromosome 19 at 58,042,352 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7144 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045328-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.