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Strain Name:
C57BL/6J-MtgxR7170Btlr/Mmmh
Stock Number:
045332-MU
Citation ID:
RRID:MMRRC_045332-MU
Other Names:
R7170 (G1)
Major Collection:

Strain Information

Nedd4
Name: neural precursor cell expressed, developmentally down-regulated 4
Synonyms: Nedd4-1, Nedd4, Nedd4a, E430025J12Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17999
HGNC: HGNC:7727
Homologene: 134533
Slc32a1
Name: solute carrier family 32 (GABA vesicular transporter), member 1
Synonyms: R75019, VGAT, Viaat
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22348
Homologene: 56451
Apoa5
Name: apolipoprotein A-V
Synonyms: RAP3, Apoav, 1300007O05Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 66113
Homologene: 14197
Emx1
Name: empty spiracles homeobox 1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 13796
HGNC: HGNC:3340
Homologene: 55799
Nsd1
Name: nuclear receptor-binding SET-domain protein 1
Synonyms: KMT3B
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 18193
VEGA: 13
Homologene: 32543
Mta3
Name: metastasis associated 3
Synonyms: 1110002J22Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 116871
Homologene: 14282
Fubp3
Name: far upstream element (FUSE) binding protein 3
Synonyms: FBP3, A330051M14Rik, Marta2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 320267
HGNC: HGNC:4005
Homologene: 45954
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 5,602,396 bp
  • A to G, chromosome 1 at 52,662,388 bp
  • A to T, chromosome 1 at 53,066,395 bp
  • G to A, chromosome 1 at 82,715,909 bp
  • A to G, chromosome 1 at 83,265,985 bp
  • G to A, chromosome 1 at 93,193,982 bp
  • A to G, chromosome 1 at 160,645,365 bp
  • A to T, chromosome 2 at 28,985,842 bp
  • A to G, chromosome 2 at 31,598,620 bp
  • A to C, chromosome 2 at 76,767,352 bp
  • C to T, chromosome 2 at 87,737,712 bp
  • A to G, chromosome 2 at 89,880,841 bp
  • A to T, chromosome 2 at 89,881,053 bp
  • T to C, chromosome 2 at 158,611,323 bp
  • T to A, chromosome 3 at 88,102,063 bp
  • T to A, chromosome 3 at 93,148,713 bp
  • A to G, chromosome 4 at 35,083,839 bp
  • A to G, chromosome 4 at 44,040,361 bp
  • A to T, chromosome 4 at 76,071,962 bp
  • A to G, chromosome 4 at 130,313,970 bp
  • C to G, chromosome 5 at 3,968,745 bp
  • T to A, chromosome 5 at 27,498,823 bp
  • A to T, chromosome 5 at 86,237,619 bp
  • A to T, chromosome 5 at 122,555,533 bp
  • A to G, chromosome 5 at 135,367,511 bp
  • A to T, chromosome 5 at 137,463,494 bp
  • T to C, chromosome 6 at 6,956,043 bp
  • C to T, chromosome 6 at 12,352,091 bp
  • T to A, chromosome 6 at 18,868,390 bp
  • G to A, chromosome 6 at 85,188,001 bp
  • C to T, chromosome 6 at 113,413,878 bp
  • A to T, chromosome 6 at 120,973,733 bp
  • T to C, chromosome 6 at 122,452,738 bp
  • T to C, chromosome 7 at 44,542,148 bp
  • T to C, chromosome 7 at 123,499,807 bp
  • T to C, chromosome 7 at 143,896,365 bp
  • A to G, chromosome 8 at 85,350,485 bp
  • T to C, chromosome 8 at 123,271,206 bp
  • T to C, chromosome 9 at 16,006,574 bp
  • T to C, chromosome 9 at 31,170,443 bp
  • T to C, chromosome 9 at 44,810,721 bp
  • T to C, chromosome 9 at 46,270,139 bp
  • T to A, chromosome 9 at 69,373,190 bp
  • T to A, chromosome 9 at 72,670,099 bp
  • C to A, chromosome 9 at 105,143,401 bp
  • C to T, chromosome 9 at 119,945,541 bp
  • G to T, chromosome 9 at 121,978,235 bp
  • C to T, chromosome 10 at 86,713,503 bp
  • C to A, chromosome 10 at 123,171,195 bp
  • T to A, chromosome 10 at 128,040,121 bp
  • T to C, chromosome 11 at 85,495,918 bp
  • T to C, chromosome 11 at 106,202,478 bp
  • T to C, chromosome 11 at 109,945,828 bp
  • A to G, chromosome 11 at 119,452,575 bp
  • C to A, chromosome 11 at 120,362,509 bp
  • T to C, chromosome 12 at 4,249,722 bp
  • T to C, chromosome 12 at 70,044,239 bp
  • T to C, chromosome 12 at 78,683,889 bp
  • T to A, chromosome 12 at 104,389,518 bp
  • T to A, chromosome 13 at 55,261,626 bp
  • A to T, chromosome 13 at 67,294,177 bp
  • T to C, chromosome 14 at 47,706,410 bp
  • A to C, chromosome 14 at 62,587,180 bp
  • G to T, chromosome 15 at 76,500,021 bp
  • T to C, chromosome 16 at 28,445,179 bp
  • G to T, chromosome 16 at 58,615,728 bp
  • A to G, chromosome 16 at 94,122,700 bp
  • G to T, chromosome 17 at 19,531,971 bp
  • C to T, chromosome 17 at 22,375,135 bp
  • T to C, chromosome 17 at 31,848,772 bp
  • G to A, chromosome 17 at 43,446,138 bp
  • G to A, chromosome 17 at 46,648,255 bp
  • C to T, chromosome 17 at 83,714,662 bp
  • T to G, chromosome 18 at 12,404,076 bp
  • T to C, chromosome 18 at 37,756,664 bp
  • T to C, chromosome 18 at 69,633,578 bp
  • A to G, chromosome 19 at 18,713,373 bp
  • A to T, chromosome 19 at 40,326,129 bp
  • A to T, chromosome 19 at 45,078,416 bp
  • A to G, chromosome X at 112,644,345 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7170 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045332-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.