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Strain Name:
C57BL/6J-MtgxR7194Btlr/Mmmh
Stock Number:
045335-MU
Citation ID:
RRID:MMRRC_045335-MU
Other Names:
R7194 (G1)
Major Collection:

Strain Information

Hmox1
Name: heme oxygenase 1
Synonyms: Hsp32, HO-1, heme oxygenase 1, D8Wsu38e, Hmox, HO1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 15368
HGNC: HGNC:5013
Homologene: 31075
Col3a1
Name: collagen, type III, alpha 1
Synonyms: Col3a-1, Tsk-2, Tsk2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12825
HGNC: HGNC:2201
Homologene: 55433
App
Name: amyloid beta precursor protein
Synonyms: betaAPP, Abeta, protease nexin II, Adap, Cvap, appican, E030013M08Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 11820
VEGA: 16
HGNC: HGNC:620
Homologene: 56379
Slc17a6
Name: solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6
Synonyms: 2900073D12Rik, VGLUT2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 140919
Homologene: 121617
Ppp4c
Name: protein phosphatase 4, catalytic subunit
Synonyms: PPX, 1110002D08Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 56420
HGNC: HGNC:9319
Homologene: 2038
Gpr139
Name: G protein-coupled receptor 139
Synonyms: LOC209776, GPRg1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 209776
Homologene: 45860
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 45,331,700 bp
  • A to G, chromosome 1 at 71,602,323 bp
  • A to T, chromosome 1 at 72,659,033 bp
  • A to T, chromosome 1 at 84,794,222 bp
  • T to C, chromosome 1 at 172,119,944 bp
  • C to A, chromosome 1 at 172,280,627 bp
  • T to G, chromosome 1 at 173,729,924 bp
  • C to G, chromosome 1 at 190,944,997 bp
  • T to A, chromosome 1 at 192,125,961 bp
  • C to A, chromosome 2 at 130,806,788 bp
  • G to T, chromosome 3 at 38,888,884 bp
  • T to A, chromosome 3 at 38,983,895 bp
  • T to A, chromosome 3 at 94,955,143 bp
  • T to G, chromosome 3 at 129,855,059 bp
  • T to A, chromosome 3 at 133,348,192 bp
  • A to G, chromosome 4 at 59,213,210 bp
  • T to A, chromosome 4 at 60,740,735 bp
  • G to A, chromosome 4 at 135,474,421 bp
  • A to T, chromosome 4 at 144,162,599 bp
  • T to A, chromosome 5 at 109,292,783 bp
  • G to A, chromosome 5 at 120,675,492 bp
  • A to T, chromosome 6 at 18,879,343 bp
  • T to C, chromosome 6 at 27,618,487 bp
  • C to T, chromosome 6 at 41,141,595 bp
  • A to G, chromosome 6 at 54,286,177 bp
  • G to T, chromosome 6 at 68,632,044 bp
  • T to G, chromosome 6 at 96,164,775 bp
  • A to G, chromosome 7 at 3,824,793 bp
  • C to A, chromosome 7 at 20,510,513 bp
  • T to A, chromosome 7 at 51,626,892 bp
  • G to A, chromosome 7 at 105,435,345 bp
  • T to A, chromosome 7 at 107,722,980 bp
  • C to T, chromosome 7 at 119,144,673 bp
  • T to C, chromosome 7 at 126,786,481 bp
  • A to G, chromosome 8 at 21,656,597 bp
  • A to G, chromosome 8 at 24,651,852 bp
  • A to G, chromosome 8 at 40,796,412 bp
  • A to G, chromosome 8 at 60,526,622 bp
  • C to A, chromosome 8 at 71,482,363 bp
  • T to C, chromosome 8 at 75,096,923 bp
  • A to T, chromosome 8 at 109,959,845 bp
  • A to G, chromosome 9 at 18,674,454 bp
  • G to A, chromosome 9 at 39,647,091 bp
  • T to C, chromosome 9 at 57,022,286 bp
  • C to T, chromosome 9 at 78,367,720 bp
  • T to C, chromosome 10 at 5,110,859 bp
  • A to G, chromosome 10 at 58,476,769 bp
  • A to G, chromosome 11 at 53,265,938 bp
  • C to T, chromosome 11 at 61,450,857 bp
  • G to A, chromosome 11 at 67,211,357 bp
  • C to T, chromosome 11 at 97,673,568 bp
  • T to A, chromosome 11 at 99,374,404 bp
  • A to G, chromosome 11 at 106,324,236 bp
  • A to T, chromosome 11 at 120,618,973 bp
  • A to G, chromosome 12 at 70,194,320 bp
  • A to C, chromosome 13 at 38,209,899 bp
  • A to T, chromosome 14 at 39,472,478 bp
  • T to A, chromosome 14 at 61,210,089 bp
  • T to C, chromosome 15 at 44,529,116 bp
  • T to C, chromosome 15 at 82,391,768 bp
  • A to C, chromosome 15 at 98,843,833 bp
  • T to C, chromosome 15 at 101,542,756 bp
  • T to C, chromosome 16 at 56,007,188 bp
  • C to T, chromosome 16 at 56,562,371 bp
  • C to T, chromosome 16 at 85,025,431 bp
  • A to T, chromosome 17 at 91,088,961 bp
  • A to T, chromosome 18 at 9,848,248 bp
  • T to A, chromosome 19 at 37,174,752 bp
  • T to C, chromosome 19 at 41,871,540 bp
  • A to T, chromosome Y at 1,304,672 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7194 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045335-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.