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Strain Name:
C57BL/6J-MtgxR7290Btlr/Mmmh
Stock Number:
045362-MU
Citation ID:
RRID:MMRRC_045362-MU
Other Names:
R7290 (G1)
Major Collection:

Strain Information

Kifc2
Name: kinesin family member C2
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 16581
VEGA: 15
Homologene: 7800
Lamb1
Name: laminin B1
Synonyms: Lamb-1, C81607, C80098, D130003D08Rik, Lamb1-1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 16777
HGNC: HGNC:6486
Homologene: 1722
Cadps
Name: Ca2+-dependent secretion activator
Synonyms: CAPS1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 27062
VEGA: 14
HGNC: HGNC:1426
Homologene: 2755
Itm2b
Name: integral membrane protein 2B
Synonyms: D14Sel6, Bri2, Bricd2b
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 16432
VEGA: 14
HGNC: HGNC:6174
Homologene: 7388
Tlx3
Name: T cell leukemia, homeobox 3
Synonyms: Tlx1l2, Rnx, Hox11l2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 27140
Homologene: 23181
Ptdss2
Name: phosphatidylserine synthase 2
Synonyms: PSS2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 27388
Homologene: 8462
Dgka
Name: diacylglycerol kinase, alpha
Synonyms: Dagk1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 13139
VEGA: 10
HGNC: HGNC:2849
Homologene: 1028
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 34,462,811 bp
  • G to A, chromosome 1 at 39,539,939 bp
  • A to T, chromosome 1 at 54,548,066 bp
  • A to T, chromosome 1 at 66,601,197 bp
  • G to T, chromosome 1 at 116,221,889 bp
  • G to A, chromosome 1 at 116,821,191 bp
  • A to G, chromosome 1 at 152,544,395 bp
  • A to G, chromosome 1 at 172,072,806 bp
  • A to T, chromosome 1 at 173,529,531 bp
  • G to A, chromosome 1 at 181,628,174 bp
  • T to C, chromosome 2 at 130,141,190 bp
  • A to T, chromosome 2 at 155,992,762 bp
  • A to G, chromosome 3 at 57,672,924 bp
  • A to T, chromosome 3 at 59,097,726 bp
  • G to A, chromosome 3 at 93,796,848 bp
  • G to A, chromosome 3 at 98,162,941 bp
  • T to A, chromosome 4 at 19,880,060 bp
  • T to C, chromosome 4 at 24,517,139 bp
  • C to T, chromosome 4 at 127,123,501 bp
  • G to T, chromosome 5 at 138,807,554 bp
  • A to G, chromosome 6 at 30,747,159 bp
  • G to A, chromosome 6 at 47,819,995 bp
  • T to C, chromosome 6 at 101,151,245 bp
  • A to G, chromosome 7 at 23,660,623 bp
  • T to A, chromosome 7 at 64,372,486 bp
  • G to T, chromosome 7 at 130,729,373 bp
  • T to C, chromosome 7 at 141,151,780 bp
  • G to A, chromosome 8 at 79,189,813 bp
  • C to A, chromosome 8 at 91,115,041 bp
  • T to A, chromosome 8 at 93,534,683 bp
  • T to C, chromosome 8 at 128,476,296 bp
  • T to C, chromosome 9 at 30,902,610 bp
  • A to G, chromosome 9 at 39,047,398 bp
  • A to T, chromosome 9 at 77,457,839 bp
  • A to T, chromosome 9 at 80,136,515 bp
  • A to T, chromosome 9 at 82,871,293 bp
  • T to G, chromosome 10 at 23,950,400 bp
  • T to C, chromosome 10 at 60,376,841 bp
  • T to G, chromosome 10 at 88,174,494 bp
  • T to C, chromosome 10 at 128,733,599 bp
  • A to T, chromosome 11 at 3,998,093 bp
  • G to A, chromosome 11 at 33,203,514 bp
  • A to G, chromosome 11 at 36,023,471 bp
  • A to G, chromosome 11 at 50,196,981 bp
  • A to G, chromosome 11 at 50,900,743 bp
  • C to A, chromosome 11 at 75,493,957 bp
  • A to T, chromosome 11 at 84,521,877 bp
  • A to G, chromosome 11 at 87,611,237 bp
  • G to A, chromosome 11 at 98,378,219 bp
  • T to A, chromosome 11 at 101,258,949 bp
  • T to A, chromosome 11 at 110,030,888 bp
  • G to T, chromosome 11 at 115,786,256 bp
  • T to C, chromosome 11 at 115,804,789 bp
  • G to A, chromosome 12 at 16,711,738 bp
  • T to A, chromosome 12 at 31,265,596 bp
  • A to G, chromosome 12 at 84,941,945 bp
  • T to C, chromosome 12 at 115,742,647 bp
  • T to C, chromosome 13 at 55,161,449 bp
  • G to T, chromosome 13 at 99,954,699 bp
  • G to A, chromosome 13 at 103,862,326 bp
  • C to A, chromosome 13 at 104,187,433 bp
  • C to A, chromosome 13 at 111,768,111 bp
  • A to T, chromosome 14 at 12,616,099 bp
  • T to A, chromosome 14 at 27,438,653 bp
  • T to C, chromosome 14 at 70,925,940 bp
  • C to T, chromosome 14 at 73,368,345 bp
  • A to G, chromosome 15 at 11,277,366 bp
  • A to T, chromosome 15 at 58,098,651 bp
  • A to T, chromosome 15 at 76,660,704 bp
  • T to C, chromosome 15 at 89,144,308 bp
  • A to T, chromosome 15 at 90,967,229 bp
  • G to A, chromosome 15 at 101,231,538 bp
  • T to A, chromosome 16 at 16,910,955 bp
  • A to T, chromosome 16 at 18,564,056 bp
  • A to G, chromosome 16 at 73,004,520 bp
  • G to A, chromosome 17 at 35,618,869 bp
  • A to T, chromosome 18 at 31,243,168 bp
  • A to G, chromosome 18 at 44,837,491 bp
  • G to T, chromosome 19 at 46,623,437 bp
  • T to C, chromosome 19 at 54,046,404 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7290 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045362-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.