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Strain Name:
C57BL/6J-MtgxR7315Btlr/Mmmh
Stock Number:
045368-MU
Citation ID:
RRID:MMRRC_045368-MU
Other Names:
R7315 (G1)
Major Collection:

Strain Information

Kitl
Name: kit ligand
Synonyms: SF, stem cell factor, Steel factor, SLF, Mgf, Sl, Gb, grizzle-belly, Steel, SCF, Kitlg, blz
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 17311
HGNC: HGNC:6343
Homologene: 692
B4galt5
Name: UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 5
Synonyms: 9430078I07Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 56336
HGNC: HGNC:928
Homologene: 3507
Ext1
Name: exostosin glycosyltransferase 1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 14042
HGNC: HGNC:3512
Homologene: 30957
Gon4l
Name: gon-4 like
Synonyms: 1500041I23Rik, 2610100B20Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 76022
Homologene: 13002
Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: Megalin, Gp330, D230004K18Rik, b2b1625.2Clo
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952
Dscaml1
Name: DS cell adhesion molecule like 1
Synonyms: 4930435C18Rik, 4921507G06Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 114873
VEGA: 9
Homologene: 79549
Nmt1
Name: N-myristoyltransferase 1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18107
HGNC: HGNC:7857
Homologene: 69027
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 120,227,912 bp
  • A to G, chromosome 1 at 144,248,899 bp
  • T to C, chromosome 1 at 155,129,290 bp
  • T to C, chromosome 2 at 5,339,230 bp
  • C to A, chromosome 2 at 29,734,492 bp
  • T to C, chromosome 2 at 69,491,822 bp
  • T to C, chromosome 2 at 111,780,659 bp
  • A to G, chromosome 2 at 148,442,541 bp
  • C to A, chromosome 2 at 150,570,583 bp
  • A to G, chromosome 2 at 150,950,050 bp
  • A to G, chromosome 2 at 167,301,376 bp
  • A to G, chromosome 3 at 79,506,205 bp
  • C to T, chromosome 3 at 86,998,113 bp
  • T to A, chromosome 3 at 88,895,179 bp
  • G to A, chromosome 3 at 145,431,870 bp
  • T to A, chromosome 4 at 43,696,961 bp
  • A to T, chromosome 4 at 126,882,592 bp
  • C to T, chromosome 4 at 133,487,084 bp
  • G to A, chromosome 4 at 139,301,870 bp
  • T to C, chromosome 4 at 155,463,019 bp
  • T to G, chromosome 4 at 156,241,360 bp
  • A to T, chromosome 6 at 29,379,363 bp
  • T to G, chromosome 6 at 38,295,147 bp
  • C to T, chromosome 6 at 73,084,760 bp
  • A to G, chromosome 6 at 145,815,751 bp
  • A to G, chromosome 7 at 49,548,289 bp
  • T to C, chromosome 7 at 86,664,237 bp
  • A to C, chromosome 7 at 120,294,118 bp
  • T to C, chromosome 7 at 141,690,402 bp
  • T to A, chromosome 8 at 9,987,970 bp
  • G to A, chromosome 8 at 31,164,738 bp
  • A to G, chromosome 8 at 33,581,516 bp
  • A to T, chromosome 8 at 91,076,012 bp
  • T to C, chromosome 8 at 107,081,217 bp
  • T to A, chromosome 8 at 124,794,330 bp
  • G to T, chromosome 9 at 19,845,835 bp
  • T to C, chromosome 9 at 37,730,576 bp
  • A to T, chromosome 9 at 37,855,247 bp
  • G to A, chromosome 9 at 45,745,125 bp
  • C to T, chromosome 9 at 56,925,867 bp
  • A to G, chromosome 9 at 104,316,224 bp
  • A to T, chromosome 9 at 116,109,738 bp
  • G to A, chromosome 10 at 100,016,112 bp
  • T to A, chromosome 10 at 116,362,379 bp
  • A to G, chromosome 11 at 3,976,085 bp
  • G to A, chromosome 11 at 34,069,906 bp
  • G to C, chromosome 11 at 69,946,433 bp
  • A to G, chromosome 11 at 102,356,484 bp
  • A to G, chromosome 11 at 103,060,183 bp
  • G to A, chromosome 11 at 104,515,444 bp
  • A to G, chromosome 11 at 116,849,102 bp
  • T to G, chromosome 11 at 117,723,528 bp
  • A to G, chromosome 12 at 36,390,374 bp
  • A to G, chromosome 12 at 87,819,703 bp
  • A to T, chromosome 13 at 32,972,257 bp
  • T to C, chromosome 14 at 8,256,139 bp
  • T to C, chromosome 14 at 34,071,547 bp
  • C to T, chromosome 14 at 52,121,001 bp
  • C to T, chromosome 14 at 65,931,440 bp
  • T to C, chromosome 15 at 53,073,387 bp
  • C to T, chromosome 15 at 89,814,338 bp
  • A to G, chromosome 16 at 59,202,133 bp
  • A to T, chromosome 16 at 63,552,609 bp
  • C to T, chromosome 17 at 12,210,298 bp
  • C to T, chromosome 17 at 23,945,306 bp
  • G to A, chromosome 17 at 24,894,866 bp
  • T to A, chromosome 17 at 32,058,571 bp
  • T to A, chromosome 17 at 34,841,169 bp
  • G to T, chromosome 17 at 37,362,660 bp
  • T to C, chromosome 17 at 46,551,794 bp
  • T to C, chromosome 17 at 71,080,897 bp
  • C to A, chromosome 17 at 84,696,714 bp
  • A to T, chromosome 18 at 20,579,160 bp
  • A to G, chromosome 18 at 46,876,984 bp
  • T to C, chromosome 19 at 32,639,225 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7315 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045368-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.