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Strain Name:
C57BL/6J-MtgxR7345Btlr/Mmmh
Stock Number:
045379-MU
Citation ID:
RRID:MMRRC_045379-MU
Other Names:
R7345 (G1)
Major Collection:

Strain Information

Mapk8
Name: mitogen-activated protein kinase 8
Synonyms: JNK1, c-Jun N-terminal kinase, Prkm8
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 26419
HGNC: HGNC:6881
Homologene: 56760
Map2k6
Name: mitogen-activated protein kinase kinase 6
Synonyms: MAP kinase kinase 6, MKK6, SAPKK3, Prkmk6
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 26399
HGNC: HGNC:6846
Homologene: 55686
Anks3
Name: ankyrin repeat and sterile alpha motif domain containing 3
Synonyms: 2700067D09Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 72615
VEGA: 16
Homologene: 12475
Stx1a
Name: syntaxin 1A (brain)
Synonyms: HPC-1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 20907
Homologene: 37941
Timeless
Name: timeless circadian clock 1
Synonyms: tim
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 21853
Homologene: 31206
Med13l
Name: mediator complex subunit 13-like
Synonyms: 2210413I17Rik, 6330591G05Rik, Trap240L, 9030618F05Rik, Thrap2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 76199
Homologene: 25256
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 75,384,835 bp
  • T to C, chromosome 2 at 30,340,601 bp
  • C to A, chromosome 2 at 38,960,127 bp
  • C to A, chromosome 2 at 129,304,773 bp
  • C to T, chromosome 2 at 158,217,209 bp
  • T to C, chromosome 3 at 61,003,674 bp
  • T to C, chromosome 3 at 61,364,118 bp
  • C to T, chromosome 3 at 125,714,659 bp
  • T to C, chromosome 4 at 43,173,966 bp
  • T to C, chromosome 4 at 43,428,022 bp
  • T to C, chromosome 4 at 45,342,583 bp
  • T to C, chromosome 4 at 88,628,179 bp
  • C to T, chromosome 4 at 135,546,246 bp
  • C to A, chromosome 4 at 154,267,315 bp
  • T to C, chromosome 5 at 15,527,904 bp
  • T to C, chromosome 5 at 77,349,119 bp
  • T to C, chromosome 5 at 100,385,270 bp
  • T to A, chromosome 5 at 110,303,903 bp
  • T to C, chromosome 5 at 118,742,760 bp
  • T to A, chromosome 5 at 135,037,188 bp
  • G to T, chromosome 5 at 137,621,261 bp
  • A to T, chromosome 6 at 124,318,938 bp
  • T to C, chromosome 7 at 3,695,615 bp
  • G to A, chromosome 7 at 12,416,595 bp
  • T to A, chromosome 7 at 16,796,160 bp
  • G to A, chromosome 7 at 19,394,889 bp
  • A to C, chromosome 7 at 99,346,356 bp
  • A to T, chromosome 7 at 105,703,967 bp
  • C to T, chromosome 7 at 108,613,063 bp
  • A to T, chromosome 7 at 125,645,670 bp
  • A to G, chromosome 9 at 8,099,816 bp
  • A to G, chromosome 9 at 39,060,579 bp
  • G to A, chromosome 9 at 39,078,334 bp
  • C to T, chromosome 10 at 128,249,754 bp
  • T to C, chromosome 11 at 19,924,958 bp
  • T to C, chromosome 11 at 80,096,006 bp
  • G to A, chromosome 11 at 97,466,478 bp
  • A to C, chromosome 11 at 98,042,613 bp
  • A to T, chromosome 11 at 110,492,908 bp
  • G to T, chromosome 11 at 115,786,256 bp
  • C to T, chromosome 13 at 13,980,480 bp
  • A to C, chromosome 13 at 65,152,743 bp
  • A to G, chromosome 13 at 96,396,796 bp
  • T to A, chromosome 14 at 14,955,503 bp
  • A to T, chromosome 14 at 33,408,111 bp
  • C to T, chromosome 14 at 55,635,531 bp
  • A to G, chromosome 14 at 75,042,353 bp
  • A to T, chromosome 15 at 68,105,043 bp
  • A to G, chromosome 15 at 102,162,578 bp
  • A to G, chromosome 16 at 4,955,910 bp
  • G to A, chromosome 17 at 32,486,779 bp
  • A to G, chromosome 17 at 35,864,584 bp
  • T to C, chromosome 18 at 37,491,923 bp
  • A to G, chromosome 18 at 71,378,824 bp
  • A to G, chromosome 18 at 74,708,024 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7345 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045379-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.