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Strain Name:
C57BL/6J-MtgxR7322Btlr/Mmmh
Stock Number:
045417-MU
Citation ID:
RRID:MMRRC_045417-MU
Other Names:
R7322 (G1)
Major Collection:

Strain Information

Epb41l1
Name: erythrocyte membrane protein band 4.1 like 1
Synonyms: 4.1N, Epb4.1l1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 13821
HGNC: HGNC:3378
Homologene: 8126
Pdzd2
Name: PDZ domain containing 2
Synonyms: 4930537L06Rik, LOC223364, A930022H17Rik, Pdzk3, Gm21706
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 68070
Homologene: 23393
Mrps6
Name: mitochondrial ribosomal protein S6
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 121022
Homologene: 41871
Rapgef2
Name: Rap guanine nucleotide exchange factor (GEF) 2
Synonyms: 5830453M24Rik, Pdzgef1, RA-GEF-1, CNRasGEF, nRapGEP
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 76089
Homologene: 35477
Map3k4
Name: mitogen-activated protein kinase kinase kinase 4
Synonyms: MTK1, MAPKKK4, D17Rp17, RP17, D17Rp17e, Mekk4, T-associated sex reversal, Tas
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 26407
VEGA: 17
HGNC: HGNC:6856
Homologene: 31346
Stk17b
Name: serine/threonine kinase 17b (apoptosis-inducing)
Synonyms: Drak2, 3110009A03Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 98267
Homologene: 31231
Tnrc6a
Name: trinucleotide repeat containing 6a
Synonyms: CAGH26, 3110054G10Rik, D130023A07Rik, 2010321I05Rik, Tnrc6
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233833
Homologene: 41399
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 51,473,070 bp
  • A to T, chromosome 1 at 53,765,945 bp
  • A to G, chromosome 1 at 54,259,966 bp
  • A to G, chromosome 1 at 64,735,328 bp
  • C to A, chromosome 1 at 87,087,462 bp
  • C to T, chromosome 1 at 146,682,688 bp
  • G to A, chromosome 1 at 171,174,465 bp
  • A to G, chromosome 2 at 31,459,081 bp
  • T to C, chromosome 2 at 37,105,591 bp
  • T to C, chromosome 2 at 74,684,011 bp
  • C to T, chromosome 2 at 86,332,564 bp
  • A to T, chromosome 2 at 87,048,479 bp
  • A to C, chromosome 2 at 125,479,195 bp
  • C to T, chromosome 2 at 131,053,694 bp
  • A to T, chromosome 2 at 156,503,851 bp
  • A to T, chromosome 2 at 164,910,821 bp
  • T to A, chromosome 2 at 167,451,302 bp
  • T to C, chromosome 3 at 54,370,280 bp
  • A to T, chromosome 3 at 79,145,823 bp
  • A to T, chromosome 4 at 48,083,238 bp
  • A to T, chromosome 4 at 53,067,151 bp
  • A to T, chromosome 4 at 131,989,719 bp
  • T to C, chromosome 4 at 136,896,154 bp
  • A to G, chromosome 4 at 150,934,337 bp
  • A to T, chromosome 5 at 65,383,364 bp
  • A to G, chromosome 5 at 124,821,269 bp
  • T to C, chromosome 6 at 126,533,791 bp
  • T to C, chromosome 7 at 5,308,645 bp
  • T to C, chromosome 7 at 43,311,138 bp
  • C to T, chromosome 7 at 99,691,848 bp
  • T to C, chromosome 7 at 100,877,797 bp
  • T to A, chromosome 7 at 103,321,287 bp
  • T to A, chromosome 7 at 123,171,508 bp
  • T to C, chromosome 7 at 127,929,563 bp
  • A to T, chromosome 8 at 11,615,841 bp
  • T to C, chromosome 8 at 13,595,857 bp
  • A to C, chromosome 8 at 71,537,207 bp
  • G to A, chromosome 9 at 21,110,204 bp
  • G to A, chromosome 9 at 108,126,421 bp
  • A to G, chromosome 10 at 79,975,695 bp
  • A to T, chromosome 10 at 127,545,564 bp
  • T to C, chromosome 11 at 43,212,547 bp
  • T to C, chromosome 11 at 78,348,623 bp
  • T to C, chromosome 11 at 82,116,582 bp
  • T to A, chromosome 11 at 88,081,584 bp
  • T to A, chromosome 12 at 75,984,024 bp
  • G to A, chromosome 12 at 83,950,668 bp
  • T to C, chromosome 12 at 105,604,304 bp
  • C to A, chromosome 12 at 115,920,667 bp
  • C to T, chromosome 13 at 9,690,409 bp
  • C to A, chromosome 13 at 59,726,976 bp
  • T to A, chromosome 13 at 100,299,388 bp
  • A to G, chromosome 14 at 7,448,178 bp
  • T to A, chromosome 14 at 51,101,411 bp
  • T to A, chromosome 14 at 70,968,391 bp
  • T to C, chromosome 15 at 12,437,162 bp
  • T to C, chromosome 15 at 77,961,676 bp
  • A to G, chromosome 16 at 18,070,907 bp
  • G to A, chromosome 16 at 66,882,846 bp
  • C to A, chromosome 16 at 92,058,447 bp
  • A to T, chromosome 16 at 96,066,119 bp
  • A to T, chromosome 17 at 12,270,946 bp
  • A to G, chromosome 17 at 33,215,699 bp
  • A to G, chromosome 17 at 53,677,057 bp
  • A to C, chromosome 17 at 57,302,266 bp
  • A to T, chromosome 17 at 79,621,611 bp
  • G to A, chromosome 18 at 22,105,549 bp
  • A to G, chromosome 18 at 84,095,135 bp
  • A to G, chromosome 19 at 44,157,690 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7322 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045417-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.