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Strain Name:
C57BL/6J-MtgxR7325Btlr/Mmmh
Stock Number:
045419-MU
Citation ID:
RRID:MMRRC_045419-MU
Other Names:
R7325 (G1)
Major Collection:

Strain Information

Rgs9
Name: regulator of G-protein signaling 9
Synonyms: Rgs9-2, RGS9-1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 19739
Homologene: 2845
Slc17a6
Name: solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6
Synonyms: 2900073D12Rik, VGLUT2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 140919
Homologene: 121617
Upp1
Name: uridine phosphorylase 1
Synonyms: UdRPase, UPase, Up
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 22271
Homologene: 2524
Mtmr14
Name: myotubularin related protein 14
Synonyms: 1110061O04Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 97287
Homologene: 11203
Snapc3
Name: small nuclear RNA activating complex, polypeptide 3
Synonyms: 5031401C21Rik, 4930558A07Rik, 1810020H02Rik, E030018J20Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 77634
Homologene: 31130
Fbxo15
Name: F-box protein 15
Synonyms: Fbx15, ecat3
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 50764
VEGA: 18
Homologene: 17644
Fip1l1
Name: factor interacting with PAPOLA and CPSF1
Synonyms: Rje, 1300019H17Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 66899
Homologene: 136254
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 25,532,630 bp
  • T to C, chromosome 1 at 55,304,598 bp
  • A to G, chromosome 1 at 121,306,937 bp
  • G to A, chromosome 1 at 171,174,465 bp
  • A to G, chromosome 2 at 86,333,000 bp
  • A to G, chromosome 2 at 119,520,139 bp
  • T to C, chromosome 2 at 120,724,704 bp
  • T to C, chromosome 2 at 139,757,043 bp
  • T to A, chromosome 2 at 150,916,166 bp
  • T to C, chromosome 3 at 87,713,292 bp
  • T to A, chromosome 3 at 93,203,372 bp
  • T to C, chromosome 3 at 103,321,636 bp
  • T to C, chromosome 3 at 108,702,928 bp
  • C to A, chromosome 4 at 49,490,213 bp
  • C to T, chromosome 4 at 65,542,669 bp
  • T to C, chromosome 4 at 83,435,270 bp
  • C to T, chromosome 4 at 108,017,702 bp
  • A to G, chromosome 5 at 74,536,799 bp
  • G to C, chromosome 5 at 146,158,361 bp
  • T to G, chromosome 6 at 23,409,935 bp
  • T to C, chromosome 6 at 90,022,272 bp
  • T to A, chromosome 6 at 113,269,548 bp
  • T to C, chromosome 6 at 141,989,225 bp
  • T to A, chromosome 6 at 149,020,570 bp
  • C to T, chromosome 7 at 6,316,586 bp
  • C to A, chromosome 7 at 33,944,357 bp
  • T to C, chromosome 7 at 37,479,860 bp
  • G to T, chromosome 7 at 51,645,018 bp
  • A to G, chromosome 7 at 126,871,088 bp
  • A to C, chromosome 7 at 127,025,553 bp
  • C to A, chromosome 7 at 144,411,685 bp
  • G to A, chromosome 8 at 16,058,707 bp
  • C to T, chromosome 8 at 105,955,715 bp
  • C to T, chromosome 9 at 94,712,898 bp
  • T to A, chromosome 9 at 103,085,749 bp
  • C to A, chromosome 10 at 45,589,507 bp
  • A to T, chromosome 10 at 80,723,803 bp
  • A to G, chromosome 10 at 90,941,432 bp
  • A to G, chromosome 10 at 128,022,919 bp
  • A to T, chromosome 10 at 130,362,041 bp
  • T to C, chromosome 11 at 9,134,743 bp
  • T to C, chromosome 11 at 23,419,052 bp
  • A to T, chromosome 11 at 69,940,355 bp
  • C to T, chromosome 11 at 73,888,275 bp
  • A to T, chromosome 11 at 109,276,581 bp
  • T to A, chromosome 11 at 114,925,032 bp
  • A to C, chromosome 13 at 30,381,907 bp
  • A to T, chromosome 13 at 34,050,341 bp
  • G to A, chromosome 13 at 47,041,484 bp
  • A to T, chromosome 13 at 93,472,743 bp
  • T to A, chromosome 14 at 50,866,038 bp
  • T to A, chromosome 15 at 86,033,008 bp
  • T to A, chromosome 16 at 32,884,248 bp
  • T to A, chromosome 16 at 38,321,601 bp
  • T to A, chromosome 16 at 49,005,821 bp
  • A to G, chromosome 17 at 18,303,987 bp
  • T to A, chromosome 17 at 34,632,187 bp
  • T to A, chromosome 18 at 37,145,360 bp
  • T to A, chromosome 18 at 37,343,387 bp
  • T to C, chromosome 18 at 61,736,846 bp
  • C to T, chromosome 18 at 84,959,118 bp
  • A to G, chromosome 19 at 13,364,637 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7325 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045419-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.