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Strain Name:
C57BL/6J-MtgxR7329Btlr/Mmmh
Stock Number:
045422-MU
Citation ID:
RRID:MMRRC_045422-MU
Other Names:
R7329 (G1)
Major Collection:

Strain Information

Sulf2
Name: sulfatase 2
Synonyms: 2010004N24Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 72043
Homologene: 10313
Cntnap2
Name: contactin associated protein-like 2
Synonyms: 5430425M22Rik, Caspr2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 66797
Homologene: 69159
Lamb3
Name: laminin, beta 3
Synonyms: nicein, 125kDa
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16780
HGNC: HGNC:6490
Homologene: 191
Trdmt1
Name: tRNA aspartic acid methyltransferase 1
Synonyms: Rnmt2, Dnmt2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 13434
HGNC: HGNC:2977
Homologene: 3249
Arrdc4
Name: arrestin domain containing 4
Synonyms: 2410003C09Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 66412
Homologene: 41636
Phf20
Name: PHD finger protein 20
Synonyms: 6820402O20Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228829
Homologene: 9507
Pcbp1
Name: poly(rC) binding protein 1
Synonyms: [a]CP-1, WBP17, hnRNP E1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 23983
HGNC: HGNC:8647
Homologene: 68506
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 20,547,519 bp
  • A to G, chromosome 1 at 60,217,196 bp
  • A to C, chromosome 1 at 97,750,753 bp
  • A to T, chromosome 1 at 188,553,198 bp
  • C to A, chromosome 1 at 193,320,540 bp
  • A to G, chromosome 2 at 13,468,771 bp
  • A to G, chromosome 2 at 13,516,122 bp
  • A to G, chromosome 2 at 36,568,696 bp
  • A to T, chromosome 2 at 49,942,500 bp
  • T to C, chromosome 2 at 87,857,241 bp
  • T to A, chromosome 2 at 129,285,364 bp
  • T to A, chromosome 2 at 156,304,632 bp
  • T to C, chromosome 2 at 166,117,088 bp
  • T to A, chromosome 2 at 173,009,445 bp
  • A to T, chromosome 3 at 94,402,855 bp
  • A to G, chromosome 3 at 107,896,331 bp
  • T to G, chromosome 4 at 61,227,689 bp
  • T to A, chromosome 4 at 73,744,137 bp
  • C to T, chromosome 4 at 86,779,874 bp
  • T to A, chromosome 4 at 86,841,081 bp
  • T to C, chromosome 4 at 124,856,719 bp
  • T to C, chromosome 4 at 141,843,711 bp
  • T to A, chromosome 5 at 34,829,755 bp
  • A to T, chromosome 5 at 36,616,395 bp
  • T to G, chromosome 5 at 52,756,869 bp
  • T to A, chromosome 5 at 65,263,135 bp
  • A to G, chromosome 5 at 112,440,907 bp
  • T to A, chromosome 5 at 129,716,641 bp
  • T to C, chromosome 5 at 142,904,391 bp
  • G to A, chromosome 6 at 47,271,271 bp
  • A to T, chromosome 6 at 67,954,645 bp
  • C to T, chromosome 6 at 68,571,103 bp
  • G to A, chromosome 6 at 73,144,722 bp
  • A to G, chromosome 6 at 86,525,116 bp
  • G to A, chromosome 7 at 3,739,876 bp
  • T to C, chromosome 7 at 12,991,162 bp
  • A to T, chromosome 7 at 17,090,686 bp
  • T to A, chromosome 7 at 30,235,252 bp
  • A to G, chromosome 7 at 43,351,971 bp
  • T to C, chromosome 7 at 46,656,861 bp
  • T to C, chromosome 7 at 48,872,110 bp
  • T to A, chromosome 7 at 68,741,027 bp
  • T to C, chromosome 7 at 102,900,832 bp
  • T to C, chromosome 7 at 110,049,017 bp
  • C to T, chromosome 8 at 11,226,494 bp
  • A to G, chromosome 8 at 106,913,463 bp
  • T to G, chromosome 9 at 7,011,247 bp
  • G to A, chromosome 9 at 21,342,752 bp
  • C to A, chromosome 9 at 38,378,160 bp
  • A to G, chromosome 9 at 55,214,977 bp
  • T to A, chromosome 9 at 67,760,137 bp
  • A to G, chromosome 9 at 77,776,191 bp
  • G to T, chromosome 9 at 97,461,369 bp
  • C to A, chromosome 9 at 103,449,922 bp
  • TTTACTCGTT to TTT, chromosome 10 at 32,889,896 bp
  • A to G, chromosome 10 at 100,447,419 bp
  • A to G, chromosome 11 at 68,810,191 bp
  • T to C, chromosome 11 at 72,712,208 bp
  • C to G, chromosome 11 at 73,027,047 bp
  • A to T, chromosome 11 at 88,169,866 bp
  • T to A, chromosome 11 at 94,509,247 bp
  • T to A, chromosome 11 at 99,004,246 bp
  • T to C, chromosome 11 at 106,017,377 bp
  • T to A, chromosome 11 at 108,014,277 bp
  • A to G, chromosome 11 at 121,160,732 bp
  • G to A, chromosome 12 at 75,966,984 bp
  • A to G, chromosome 12 at 83,978,082 bp
  • A to G, chromosome 13 at 32,716,783 bp
  • A to G, chromosome 14 at 47,310,463 bp
  • T to C, chromosome 14 at 80,011,929 bp
  • C to A, chromosome 15 at 36,555,476 bp
  • C to A, chromosome 15 at 74,539,245 bp
  • A to T, chromosome 16 at 16,767,019 bp
  • T to C, chromosome 16 at 90,386,682 bp
  • A to G, chromosome 17 at 20,846,690 bp
  • A to T, chromosome 17 at 56,803,681 bp
  • A to C, chromosome 18 at 36,540,821 bp
  • A to G, chromosome 19 at 9,001,792 bp
  • G to T, chromosome 19 at 10,587,904 bp
  • T to C, chromosome 19 at 58,789,222 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7329 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045422-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.