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Strain Detail Sheet

Strain Name:
C57BL/6J-MtgxR7365Btlr/Mmmh
Stock Number:
045449-MU
Citation ID:
RRID:MMRRC_045449-MU
Other Names:
R7365 (G1)
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Epha7
Name: Eph receptor A7
Synonyms: MDK1, Ebk, Cek11, Ehk3, Hek11, Mdk1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 13841
HGNC: HGNC:3390
Homologene: 20935

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Ep400
Name: E1A binding protein p400
Synonyms: p400, mDomino, 1700020J09Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 75560
Homologene: 38779

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Gene Ontology
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Mouse Phenome DB
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KOMP Phenotyping
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ClinGen
Tnpo3
Name: transportin 3
Synonyms: 5730544L10Rik, C430013M08Rik, D6Ertd313e
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 320938
Homologene: 40848

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Gene Ontology
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ClinGen
Wfs1
Name: wolframin ER transmembrane glycoprotein
Synonyms: wolframin, Wolfram syndrome 1 homolog (human)
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 22393
Homologene: 4380

Additional Resources
IMPC
Google
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Sirt1
Name: sirtuin 1
Synonyms: Sir2, Sir2alpha
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 93759
Homologene: 56556

Additional Resources
IMPC
Google
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PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
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Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Ankrd17
Name: ankyrin repeat domain 17
Synonyms: Gtar, 4933425K22Rik, A130069E23Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 81702
Homologene: 82403

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Ezh2
Name: enhancer of zeste 2 polycomb repressive complex 2 subunit
Synonyms: Enx-1, Enx1h, KMT6
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 14056
HGNC: HGNC:3527
Homologene: 37926

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 24,744,867 bp
  • A to T, chromosome 1 at 28,780,152 bp
  • A to T, chromosome 1 at 53,497,138 bp
  • T to A, chromosome 1 at 87,389,017 bp
  • C to T, chromosome 1 at 111,861,573 bp
  • C to T, chromosome 2 at 32,303,001 bp
  • A to T, chromosome 2 at 65,098,373 bp
  • A to G, chromosome 2 at 89,440,198 bp
  • A to G, chromosome 2 at 101,630,773 bp
  • A to T, chromosome 2 at 112,043,014 bp
  • A to G, chromosome 2 at 120,494,814 bp
  • G to A, chromosome 2 at 125,352,049 bp
  • A to G, chromosome 2 at 127,426,981 bp
  • A to T, chromosome 2 at 164,854,322 bp
  • C to A, chromosome 3 at 34,650,972 bp
  • A to G, chromosome 3 at 89,408,665 bp
  • A to T, chromosome 3 at 93,148,534 bp
  • C to A, chromosome 3 at 100,963,302 bp
  • C to A, chromosome 3 at 109,628,099 bp
  • T to C, chromosome 3 at 144,922,768 bp
  • T to A, chromosome 3 at 145,098,784 bp
  • T to G, chromosome 3 at 158,198,161 bp
  • C to T, chromosome 4 at 28,871,937 bp
  • A to G, chromosome 4 at 41,098,003 bp
  • T to G, chromosome 4 at 48,051,290 bp
  • A to G, chromosome 4 at 107,350,541 bp
  • T to C, chromosome 4 at 155,872,230 bp
  • A to C, chromosome 5 at 36,967,732 bp
  • A to G, chromosome 5 at 44,020,831 bp
  • C to A, chromosome 5 at 88,501,488 bp
  • T to C, chromosome 5 at 90,291,151 bp
  • T to A, chromosome 5 at 109,293,239 bp
  • A to G, chromosome 5 at 109,297,522 bp
  • T to C, chromosome 5 at 110,719,614 bp
  • T to C, chromosome 5 at 138,562,936 bp
  • A to G, chromosome 5 at 138,820,981 bp
  • A to G, chromosome 5 at 150,532,337 bp
  • A to G, chromosome 6 at 29,556,996 bp
  • G to T, chromosome 6 at 47,533,758 bp
  • A to C, chromosome 6 at 52,259,882 bp
  • A to G, chromosome 6 at 136,560,710 bp
  • T to A, chromosome 7 at 26,371,397 bp
  • T to C, chromosome 7 at 29,085,755 bp
  • G to A, chromosome 7 at 35,429,928 bp
  • T to C, chromosome 7 at 35,776,418 bp
  • T to C, chromosome 7 at 46,298,308 bp
  • T to A, chromosome 7 at 105,128,345 bp
  • A to G, chromosome 7 at 106,713,964 bp
  • T to A, chromosome 7 at 128,135,309 bp
  • C to T, chromosome 8 at 70,029,234 bp
  • T to A, chromosome 8 at 71,474,493 bp
  • T to A, chromosome 8 at 71,485,110 bp
  • A to T, chromosome 8 at 110,557,662 bp
  • A to T, chromosome 8 at 110,601,273 bp
  • T to C, chromosome 8 at 116,514,957 bp
  • A to G, chromosome 8 at 125,155,041 bp
  • A to T, chromosome 9 at 38,930,776 bp
  • T to C, chromosome 9 at 69,400,512 bp
  • T to C, chromosome 9 at 79,706,360 bp
  • A to T, chromosome 9 at 105,422,999 bp
  • A to G, chromosome 10 at 63,322,003 bp
  • T to C, chromosome 10 at 93,644,804 bp
  • G to A, chromosome 10 at 121,083,909 bp
  • G to A, chromosome 10 at 121,667,811 bp
  • A to T, chromosome 10 at 127,176,847 bp
  • A to G, chromosome 10 at 127,877,024 bp
  • T to C, chromosome 11 at 29,161,334 bp
  • T to A, chromosome 11 at 46,207,440 bp
  • A to G, chromosome 11 at 67,242,848 bp
  • T to C, chromosome 11 at 98,221,084 bp
  • G to A, chromosome 11 at 108,939,376 bp
  • G to A, chromosome 13 at 4,237,070 bp
  • A to G, chromosome 13 at 11,640,275 bp
  • T to C, chromosome 13 at 54,996,573 bp
  • T to C, chromosome 13 at 55,714,788 bp
  • G to A, chromosome 13 at 62,700,905 bp
  • G to T, chromosome 13 at 97,143,021 bp
  • T to A, chromosome 14 at 16,416,649 bp
  • A to C, chromosome 14 at 28,040,389 bp
  • A to G, chromosome 14 at 45,279,014 bp
  • G to T, chromosome 14 at 57,873,920 bp
  • A to G, chromosome 14 at 70,134,527 bp
  • T to C, chromosome 14 at 118,627,654 bp
  • A to G, chromosome 14 at 120,920,085 bp
  • T to A, chromosome 15 at 74,610,371 bp
  • G to A, chromosome 16 at 18,777,095 bp
  • A to T, chromosome 16 at 30,308,608 bp
  • T to A, chromosome 16 at 34,723,619 bp
  • T to C, chromosome 16 at 45,980,426 bp
  • T to A, chromosome 16 at 49,001,653 bp
  • CACTTAC to CAC, chromosome 16 at 90,772,573 bp
  • G to T, chromosome 17 at 25,373,210 bp
  • A to T, chromosome 17 at 33,033,378 bp
  • C to A, chromosome 18 at 7,000,747 bp
  • G to A, chromosome 18 at 71,826,123 bp
  • A to T, chromosome 19 at 44,132,140 bp
  • A to T, chromosome 19 at 60,766,644 bp

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7365 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
045449-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.