MMRRC:045465-MU

Synonyms: GA_x6K02T2NKPP-754816-754257, GA_x6K02SYUPNB-557-3, GA_x6K02SYUNE5-2-727, MOR256-64, OTTMUSG00000005755, Olfr216, Olfr322-ps1, Olfr179, Olfr322

Type: Gene

Species: Mouse

Chromosome: 11

MGI: MGI:3030156

NCBI: 404346

Ensembl: ENSMUSG00000063549

VEGA: OTTMUSG00000005755

HGNC: HGNC:15021

Homologene: 19876

CCDS: CCDS36165.1

UCSC: Chr11:58556387-58557370

Additional Resources

IMPC
Google
Entrez
PubMed
IMSR

Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB

Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen

Tnfsf12

Name: tumor necrosis factor (ligand) superfamily, member 12

Synonyms: Tweak, APO3L, DR3L

Type: Gene

Species: Mouse

Chromosome: 11

MGI: MGI:1196259

NCBI: 21944

Ensembl: ENSMUSG00000097328

VEGA: OTTMUSG00000005999

HGNC: HGNC:33537|HGNC:11927

Homologene: 7979

CCDS: CCDS24907.1

UCSC: Chr11:69577066-69586924

Additional Resources

IMPC
Google
Entrez
PubMed
IMSR

Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB

Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen

Genetic Alterations

ENU-induced transitions at the following base pair locations (GRCm38):

G to A, chromosome 1 at 84,912,571 bp
A to T, chromosome 1 at 92,464,461 bp
A to T, chromosome 1 at 97,075,883 bp
A to G, chromosome 1 at 135,388,673 bp
T to A, chromosome 1 at 143,784,015 bp
T to G, chromosome 1 at 178,530,710 bp
G to A, chromosome 2 at 30,111,241 bp
A to T, chromosome 2 at 83,915,328 bp
A to T, chromosome 2 at 84,694,404 bp
A to G, chromosome 2 at 86,676,919 bp
A to G, chromosome 2 at 87,435,377 bp
G to A, chromosome 2 at 119,960,340 bp
A to T, chromosome 2 at 129,269,252 bp
A to T, chromosome 3 at 93,331,791 bp
A to G, chromosome 3 at 145,298,838 bp
T to A, chromosome 4 at 9,859,537 bp
C to A, chromosome 4 at 11,514,026 bp
T to A, chromosome 4 at 48,636,841 bp
A to T, chromosome 4 at 117,891,369 bp
G to A, chromosome 4 at 118,365,214 bp
C to T, chromosome 4 at 149,125,404 bp
A to T, chromosome 5 at 109,092,818 bp
A to G, chromosome 6 at 32,152,799 bp
G to T, chromosome 6 at 54,485,278 bp
A to G, chromosome 6 at 82,749,295 bp
A to G, chromosome 6 at 91,987,118 bp
A to G, chromosome 6 at 122,323,358 bp
T to A, chromosome 6 at 136,840,455 bp
C to A, chromosome 7 at 10,904,033 bp
C to A, chromosome 7 at 19,585,273 bp
T to A, chromosome 7 at 30,765,368 bp
A to T, chromosome 7 at 86,694,752 bp
T to C, chromosome 7 at 88,430,429 bp
A to G, chromosome 7 at 128,694,586 bp
C to A, chromosome 8 at 22,669,050 bp
A to C, chromosome 8 at 71,603,826 bp
G to A, chromosome 8 at 85,050,119 bp
A to G, chromosome 8 at 125,447,646 bp
C to T, chromosome 9 at 45,968,849 bp
T to A, chromosome 9 at 53,562,778 bp
T to A, chromosome 9 at 70,297,295 bp
T to G, chromosome 9 at 83,953,713 bp
A to T, chromosome 10 at 67,189,758 bp
A to T, chromosome 10 at 79,531,007 bp
A to T, chromosome 10 at 84,528,284 bp
A to G, chromosome 10 at 109,716,671 bp
T to A, chromosome 11 at 5,868,548 bp
T to C, chromosome 11 at 6,217,777 bp
T to C, chromosome 11 at 58,666,185 bp
A to G, chromosome 11 at 69,687,066 bp
A to G, chromosome 11 at 73,485,889 bp
C to G, chromosome 11 at 80,223,934 bp
A to G, chromosome 11 at 106,264,776 bp
T to G, chromosome 12 at 81,315,805 bp
T to G, chromosome 12 at 85,044,468 bp
T to C, chromosome 13 at 49,268,309 bp
T to G, chromosome 14 at 49,074,196 bp
T to C, chromosome 14 at 52,215,319 bp
A to T, chromosome 14 at 55,068,253 bp
T to C, chromosome 14 at 57,917,353 bp
T to A, chromosome 15 at 79,716,153 bp
C to G, chromosome 15 at 98,456,697 bp
G to T, chromosome 16 at 5,097,971 bp
G to A, chromosome 16 at 7,070,035 bp
TTGTG to TTG, chromosome 16 at 14,300,145 bp
T to C, chromosome 16 at 64,926,843 bp
T to G, chromosome 17 at 18,440,472 bp
A to G, chromosome 17 at 37,806,081 bp
A to T, chromosome 17 at 71,700,517 bp
A to G, chromosome 17 at 88,002,854 bp
A to C, chromosome 18 at 50,164,850 bp
A to T, chromosome 19 at 10,866,994 bp

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.

Strain GQC Summary

Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice

Littermates of all relevant genotypes.

Strain Origin

Donor

Bruce Beutler, M.D., University of Texas Southwestern Medical Center.

Primary Reference

MUTAGENETIX record for R7383 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Strain Development

C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.

Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information Expand All

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color

Black

Breeding

MMRRC Breeding System

Random intra-strain mating

Breeding Scheme(s)

When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.

Overall Breeding Performance

Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size

Undetermined

Recommended wean age

3 weeks

Order Information Expand All

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)

MMRRC Item #	Description	Distribution Fee / Unit (US $) Shipping & Handling not included	Units	Notes
045465-MU-RESUS	Litter recovered from cryo-archive	$5,475.00 / Non-Profit	Litter	Recovered litter⁴; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

¹ The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

² An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

³ An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

⁴ The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.

Strain Detail Sheet

Strain Information Expand All

Colony and Husbandry Information Expand All

Order Information Expand All