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Strain Name:
C57BL/6J-MtgxR7400Btlr/Mmmh
Stock Number:
045482-MU
Citation ID:
RRID:MMRRC_045482-MU
Other Names:
R7400 (G1)
Major Collection:

Strain Information

Mmp10
Name: matrix metallopeptidase 10
Synonyms: stromelysin 2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17384
VEGA: 9
HGNC: HGNC:7156
Homologene: 20546
Slamf6
Name: SLAM family member 6
Synonyms: SF2000, NTB-A, KAL1b, KAL1, Ly108
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 30925
Homologene: 49945
Spen
Name: spen family transcription repressor
Synonyms: Mint
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 56381
Homologene: 124461
Hsp90ab1
Name: heat shock protein 90 alpha (cytosolic), class B member 1
Synonyms: Hsp84, Hsp90, Hsp84-1, C81438, Hspcb
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 15516
Homologene: 74306
Atp5mc2
Name: ATP synthase membrane subunit c locus 2
Synonyms: 1810041M08Rik, Atp5g2
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 67942
HGNC: HGNC:842
Homologene: 57052
Dock6
Name: dedicator of cytokinesis 6
Synonyms: 2410095B20Rik, 4931431C02Rik, C330023D02Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 319899
VEGA: 9
Homologene: 83291
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 19,142,926 bp
  • T to C, chromosome 1 at 60,042,642 bp
  • A to T, chromosome 1 at 133,274,024 bp
  • T to C, chromosome 1 at 150,674,430 bp
  • G to A, chromosome 1 at 171,695,925 bp
  • T to C, chromosome 1 at 171,919,793 bp
  • A to C, chromosome 1 at 179,700,274 bp
  • G to T, chromosome 2 at 25,406,245 bp
  • T to C, chromosome 2 at 68,666,203 bp
  • A to G, chromosome 2 at 117,298,545 bp
  • T to C, chromosome 2 at 118,791,886 bp
  • A to G, chromosome 2 at 131,086,095 bp
  • T to A, chromosome 2 at 156,126,323 bp
  • T to C, chromosome 2 at 180,153,321 bp
  • C to A, chromosome 3 at 38,887,924 bp
  • T to C, chromosome 3 at 88,167,731 bp
  • C to T, chromosome 3 at 89,230,646 bp
  • T to A, chromosome 3 at 108,679,688 bp
  • A to G, chromosome 3 at 154,762,577 bp
  • T to C, chromosome 4 at 141,473,741 bp
  • A to T, chromosome 5 at 21,971,934 bp
  • T to C, chromosome 5 at 30,385,188 bp
  • A to G, chromosome 5 at 65,148,590 bp
  • A to T, chromosome 6 at 41,771,744 bp
  • A to G, chromosome 6 at 52,217,053 bp
  • A to G, chromosome 6 at 138,073,057 bp
  • T to A, chromosome 7 at 10,274,947 bp
  • T to C, chromosome 7 at 15,950,656 bp
  • G to C, chromosome 7 at 63,938,248 bp
  • T to C, chromosome 7 at 96,694,803 bp
  • C to A, chromosome 7 at 98,443,640 bp
  • T to C, chromosome 7 at 103,187,380 bp
  • T to C, chromosome 7 at 105,035,210 bp
  • T to A, chromosome 7 at 105,902,040 bp
  • T to A, chromosome 7 at 145,321,906 bp
  • A to T, chromosome 8 at 99,279,560 bp
  • A to G, chromosome 8 at 117,257,405 bp
  • T to A, chromosome 9 at 7,503,300 bp
  • G to T, chromosome 9 at 21,801,807 bp
  • A to C, chromosome 9 at 31,108,275 bp
  • T to C, chromosome 9 at 57,681,940 bp
  • T to A, chromosome 9 at 70,586,070 bp
  • T to A, chromosome 9 at 103,250,662 bp
  • T to G, chromosome 9 at 108,681,973 bp
  • A to T, chromosome 10 at 5,218,580 bp
  • T to A, chromosome 10 at 42,698,217 bp
  • T to A, chromosome 10 at 95,995,570 bp
  • A to G, chromosome 11 at 32,824,471 bp
  • A to G, chromosome 11 at 94,483,400 bp
  • A to G, chromosome 11 at 99,543,143 bp
  • A to G, chromosome 11 at 115,860,113 bp
  • C to A, chromosome 12 at 64,471,617 bp
  • A to G, chromosome 12 at 103,104,630 bp
  • A to G, chromosome 12 at 115,758,217 bp
  • T to C, chromosome 13 at 34,959,977 bp
  • A to C, chromosome 14 at 20,408,267 bp
  • T to C, chromosome 14 at 31,157,384 bp
  • A to G, chromosome 14 at 53,977,563 bp
  • A to T, chromosome 14 at 55,576,769 bp
  • A to T, chromosome 15 at 35,378,900 bp
  • C to A, chromosome 15 at 85,069,720 bp
  • C to T, chromosome 15 at 102,665,112 bp
  • C to T, chromosome 16 at 21,922,322 bp
  • C to A, chromosome 16 at 94,335,900 bp
  • A to T, chromosome 17 at 28,858,976 bp
  • A to T, chromosome 17 at 38,305,331 bp
  • A to G, chromosome 17 at 45,569,284 bp
  • A to T, chromosome 18 at 12,643,799 bp
  • T to C, chromosome 18 at 37,854,502 bp
  • C to T, chromosome 18 at 65,638,913 bp
  • A to G, chromosome 19 at 6,171,508 bp
  • T to A, chromosome 19 at 9,014,613 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7400 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045482-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.