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Strain Name:
C57BL/6J-MtgxR7423Btlr/Mmmh
Stock Number:
045501-MU
Citation ID:
RRID:MMRRC_045501-MU
Other Names:
R7423 (G1)
Major Collection:

Strain Information

Pdpk1
Name: 3-phosphoinositide dependent protein kinase 1
Synonyms: Pkb kinase, Pdk1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18607
Homologene: 37643
Pofut2
Name: protein O-fucosyltransferase 2
Synonyms: FUT13, 2310011G23Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 80294
VEGA: 10
Homologene: 12724
Sbf2
Name: SET binding factor 2
Synonyms: SBF2, 4833411B01Rik, mMTMH1, B430219L04Rik, Mtmr13
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 319934
HGNC: HGNC:2135
Homologene: 41810
Pik3cd
Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta
Synonyms: p110delta, 2410099E07Rik, 2610208K16Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18707
HGNC: HGNC:8977
Homologene: 3686
Tpgs1
Name: tubulin polyglutamylase complex subunit 1
Synonyms: Gtrgeo22, Gm16517
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 110012
VEGA: 10
Homologene: 17525
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 58,121,069 bp
  • G to A, chromosome 1 at 80,523,780 bp
  • T to C, chromosome 1 at 87,786,301 bp
  • A to G, chromosome 1 at 178,329,284 bp
  • A to C, chromosome 1 at 190,799,096 bp
  • C to A, chromosome 2 at 15,068,172 bp
  • T to A, chromosome 3 at 54,318,029 bp
  • T to C, chromosome 3 at 88,309,196 bp
  • T to C, chromosome 3 at 122,275,526 bp
  • G to A, chromosome 4 at 4,076,921 bp
  • A to T, chromosome 4 at 14,825,858 bp
  • A to G, chromosome 4 at 45,256,948 bp
  • A to T, chromosome 4 at 53,686,406 bp
  • A to T, chromosome 4 at 59,076,264 bp
  • A to T, chromosome 4 at 115,790,630 bp
  • G to A, chromosome 4 at 130,774,545 bp
  • G to A, chromosome 4 at 148,556,344 bp
  • A to T, chromosome 4 at 149,651,763 bp
  • A to C, chromosome 4 at 154,983,737 bp
  • A to G, chromosome 4 at 156,230,685 bp
  • A to G, chromosome 5 at 32,150,463 bp
  • T to A, chromosome 5 at 103,847,101 bp
  • G to A, chromosome 5 at 109,297,528 bp
  • A to G, chromosome 5 at 115,134,684 bp
  • A to T, chromosome 6 at 3,374,408 bp
  • G to C, chromosome 6 at 29,468,106 bp
  • A to G, chromosome 6 at 81,946,560 bp
  • G to A, chromosome 6 at 85,830,495 bp
  • G to A, chromosome 6 at 122,079,726 bp
  • A to G, chromosome 6 at 125,128,859 bp
  • T to C, chromosome 7 at 6,393,957 bp
  • C to T, chromosome 7 at 28,894,255 bp
  • A to T, chromosome 7 at 44,014,769 bp
  • A to G, chromosome 7 at 97,507,719 bp
  • A to G, chromosome 7 at 110,438,848 bp
  • A to T, chromosome 7 at 115,550,023 bp
  • G to A, chromosome 7 at 127,489,461 bp
  • A to C, chromosome 8 at 35,804,607 bp
  • T to C, chromosome 9 at 21,036,905 bp
  • A to G, chromosome 9 at 106,967,171 bp
  • A to T, chromosome 9 at 121,103,621 bp
  • A to G, chromosome 9 at 123,964,385 bp
  • A to T, chromosome 10 at 3,547,088 bp
  • A to T, chromosome 10 at 18,093,887 bp
  • A to T, chromosome 10 at 27,212,226 bp
  • G to T, chromosome 10 at 43,022,568 bp
  • A to G, chromosome 10 at 63,048,004 bp
  • T to C, chromosome 10 at 77,262,439 bp
  • G to T, chromosome 10 at 79,675,821 bp
  • A to T, chromosome 10 at 91,059,606 bp
  • A to T, chromosome 10 at 116,492,398 bp
  • C to T, chromosome 10 at 127,357,406 bp
  • A to T, chromosome 11 at 3,531,200 bp
  • T to A, chromosome 11 at 53,068,555 bp
  • T to G, chromosome 11 at 57,529,503 bp
  • G to A, chromosome 11 at 74,121,985 bp
  • G to T, chromosome 11 at 78,194,817 bp
  • A to G, chromosome 11 at 93,943,796 bp
  • A to T, chromosome 11 at 100,135,560 bp
  • A to G, chromosome 12 at 78,900,110 bp
  • G to T, chromosome 12 at 112,609,738 bp
  • A to T, chromosome 12 at 113,597,500 bp
  • T to C, chromosome 13 at 9,878,809 bp
  • G to T, chromosome 13 at 19,190,644 bp
  • A to T, chromosome 13 at 46,696,644 bp
  • T to A, chromosome 13 at 49,038,132 bp
  • T to C, chromosome 13 at 51,123,290 bp
  • A to T, chromosome 13 at 96,048,548 bp
  • A to T, chromosome 14 at 29,997,115 bp
  • G to A, chromosome 14 at 30,571,035 bp
  • A to G, chromosome 14 at 31,171,701 bp
  • A to G, chromosome 14 at 32,628,821 bp
  • T to A, chromosome 14 at 53,946,478 bp
  • A to G, chromosome 14 at 75,049,643 bp
  • T to A, chromosome 15 at 78,292,560 bp
  • T to A, chromosome 15 at 91,572,680 bp
  • T to A, chromosome 15 at 99,329,400 bp
  • A to G, chromosome 16 at 17,258,129 bp
  • T to G, chromosome 16 at 25,422,253 bp
  • C to T, chromosome 16 at 55,233,664 bp
  • T to A, chromosome 17 at 13,361,241 bp
  • C to A, chromosome 17 at 24,110,900 bp
  • A to G, chromosome 17 at 28,648,203 bp
  • T to C, chromosome 17 at 30,704,769 bp
  • T to A, chromosome 17 at 46,726,140 bp
  • A to C, chromosome 17 at 56,414,793 bp
  • A to T, chromosome 17 at 56,868,257 bp
  • A to T, chromosome 17 at 57,214,767 bp
  • T to C, chromosome 17 at 65,858,981 bp
  • T to C, chromosome 18 at 36,667,483 bp
  • T to A, chromosome 18 at 63,777,380 bp
  • A to T, chromosome 19 at 4,621,178 bp
  • C to A, chromosome 19 at 6,910,002 bp
  • A to G, chromosome 19 at 10,664,340 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7423 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045501-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.