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Strain Name:
C57BL/6J-MtgxR7443Btlr/Mmmh
Stock Number:
045519-MU
Citation ID:
RRID:MMRRC_045519-MU
Other Names:
R7443 (G1)
Major Collection:

Strain Information

Tns3
Name: tensin 3
Synonyms: TEM6, F830010I22Rik, Tens1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 319939
Homologene: 49713
Rapgef2
Name: Rap guanine nucleotide exchange factor (GEF) 2
Synonyms: 5830453M24Rik, Pdzgef1, RA-GEF-1, CNRasGEF, nRapGEP
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 76089
Homologene: 35477
Abl2
Name: ABL proto-oncogene 2, non-receptor tyrosine kinase
Synonyms: Arg, Abll
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 11352
HGNC: HGNC:77
Homologene: 5278
Mdc1
Name: mediator of DNA damage checkpoint 1
Synonyms: NFBD1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 240087
Homologene: 67092
Tbc1d5
Name: TBC1 domain family, member 5
Synonyms: 1600014N05Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 72238
VEGA: 17
Homologene: 8834
Plcg2
Name: phospholipase C, gamma 2
Synonyms: Plcg-2, PLCgamma2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234779
HGNC: HGNC:9066
Homologene: 55671
Mccc2
Name: methylcrotonoyl-Coenzyme A carboxylase 2 (beta)
Synonyms: 4930552N12Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 78038
VEGA: 13
HGNC: HGNC:6937
Homologene: 11145
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 37,881,036 bp
  • T to C, chromosome 1 at 106,881,970 bp
  • C to A, chromosome 1 at 133,384,573 bp
  • T to C, chromosome 1 at 133,384,631 bp
  • T to A, chromosome 1 at 134,077,695 bp
  • T to C, chromosome 1 at 156,625,381 bp
  • T to A, chromosome 2 at 13,455,509 bp
  • C to A, chromosome 2 at 24,962,493 bp
  • T to A, chromosome 2 at 85,663,568 bp
  • T to A, chromosome 2 at 89,751,941 bp
  • T to A, chromosome 2 at 104,684,135 bp
  • T to C, chromosome 2 at 152,339,772 bp
  • T to G, chromosome 2 at 152,911,426 bp
  • A to G, chromosome 2 at 163,559,012 bp
  • C to A, chromosome 3 at 36,553,841 bp
  • T to C, chromosome 3 at 79,081,224 bp
  • A to G, chromosome 3 at 82,371,356 bp
  • A to T, chromosome 4 at 46,396,145 bp
  • A to T, chromosome 4 at 115,248,559 bp
  • G to A, chromosome 4 at 116,173,467 bp
  • T to C, chromosome 5 at 20,927,623 bp
  • T to A, chromosome 5 at 45,672,310 bp
  • T to C, chromosome 5 at 76,856,638 bp
  • A to G, chromosome 5 at 105,330,950 bp
  • T to A, chromosome 5 at 109,192,043 bp
  • C to A, chromosome 5 at 120,805,419 bp
  • T to C, chromosome 6 at 83,264,315 bp
  • C to T, chromosome 6 at 138,377,222 bp
  • A to G, chromosome 7 at 12,922,995 bp
  • T to A, chromosome 7 at 21,098,020 bp
  • A to G, chromosome 7 at 34,353,838 bp
  • T to A, chromosome 7 at 44,116,110 bp
  • T to A, chromosome 7 at 45,638,724 bp
  • A to G, chromosome 7 at 104,300,842 bp
  • A to T, chromosome 7 at 121,132,410 bp
  • A to G, chromosome 7 at 140,611,048 bp
  • A to G, chromosome 8 at 71,630,959 bp
  • A to G, chromosome 8 at 79,190,418 bp
  • T to C, chromosome 8 at 105,380,867 bp
  • A to T, chromosome 8 at 117,504,289 bp
  • A to G, chromosome 9 at 108,574,290 bp
  • T to C, chromosome 9 at 109,114,607 bp
  • T to C, chromosome 10 at 75,143,136 bp
  • A to T, chromosome 10 at 79,540,455 bp
  • A to T, chromosome 10 at 130,472,719 bp
  • G to A, chromosome 11 at 8,451,442 bp
  • T to C, chromosome 11 at 67,220,505 bp
  • A to G, chromosome 11 at 70,618,266 bp
  • A to T, chromosome 11 at 73,516,848 bp
  • G to A, chromosome 11 at 84,315,793 bp
  • C to A, chromosome 11 at 88,074,663 bp
  • T to A, chromosome 11 at 103,969,150 bp
  • T to C, chromosome 12 at 99,388,779 bp
  • A to G, chromosome 12 at 111,710,727 bp
  • G to T, chromosome 12 at 113,272,165 bp
  • A to T, chromosome 13 at 21,388,438 bp
  • T to A, chromosome 13 at 65,049,489 bp
  • G to A, chromosome 13 at 99,993,636 bp
  • A to T, chromosome 14 at 4,871,265 bp
  • A to G, chromosome 14 at 50,425,050 bp
  • G to A, chromosome 14 at 55,871,416 bp
  • T to C, chromosome 15 at 74,775,400 bp
  • T to A, chromosome 16 at 58,999,990 bp
  • T to C, chromosome 16 at 59,775,625 bp
  • C to T, chromosome 17 at 12,980,934 bp
  • T to A, chromosome 17 at 23,460,133 bp
  • C to T, chromosome 17 at 23,460,345 bp
  • T to A, chromosome 17 at 35,850,820 bp
  • T to A, chromosome 17 at 36,286,053 bp
  • T to C, chromosome 17 at 50,966,735 bp
  • T to C, chromosome 17 at 75,364,437 bp
  • A to T, chromosome 18 at 60,246,050 bp
  • A to G, chromosome 19 at 11,791,844 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7443 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045519-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.