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Strain Name:
C57BL/6J-MtgxR7451Btlr/Mmmh
Stock Number:
045525-MU
Citation ID:
RRID:MMRRC_045525-MU
Other Names:
R7451 (G1)
Major Collection:

Strain Information

Cacna1g
Name: calcium channel, voltage-dependent, T type, alpha 1G subunit
Synonyms: a1G, Cav3.1d
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 12291
HGNC: HGNC:1394
Homologene: 22544
Lars1
Name: leucyl-tRNA synthetase 1
Synonyms: 3110009L02Rik, 2310045K21Rik, Lars
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 107045
VEGA: 18
HGNC: HGNC:6512
Homologene: 7083
Ranbp17
Name: RAN binding protein 17
Synonyms: 4932704E15Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 66011
Homologene: 36409
Nup155
Name: nucleoporin 155
Synonyms: D930027M19Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 170762
VEGA: 15
HGNC: HGNC:8063
Homologene: 43155
Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: Megalin, Gp330, D230004K18Rik, b2b1625.2Clo
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952
Mybl2
Name: myeloblastosis oncogene-like 2
Synonyms: Bmyb, B-Myb
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 17865
HGNC: HGNC:7548
Homologene: 1847
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 75,172,153 bp
  • G to A, chromosome 1 at 119,910,241 bp
  • T to G, chromosome 1 at 173,899,492 bp
  • C to A, chromosome 1 at 192,137,876 bp
  • A to G, chromosome 2 at 25,564,461 bp
  • T to C, chromosome 2 at 39,125,136 bp
  • C to T, chromosome 2 at 52,201,454 bp
  • T to A, chromosome 2 at 60,535,002 bp
  • T to G, chromosome 2 at 69,513,333 bp
  • C to T, chromosome 2 at 76,022,773 bp
  • A to G, chromosome 2 at 89,789,109 bp
  • T to C, chromosome 2 at 122,168,027 bp
  • A to T, chromosome 2 at 126,826,737 bp
  • C to T, chromosome 2 at 163,072,706 bp
  • G to T, chromosome 2 at 163,721,163 bp
  • G to T, chromosome 3 at 37,022,807 bp
  • T to C, chromosome 3 at 92,655,900 bp
  • T to C, chromosome 3 at 129,917,103 bp
  • C to T, chromosome 4 at 45,279,558 bp
  • T to A, chromosome 4 at 63,378,667 bp
  • T to A, chromosome 4 at 117,052,796 bp
  • C to T, chromosome 4 at 118,753,687 bp
  • A to T, chromosome 4 at 130,014,557 bp
  • A to G, chromosome 4 at 155,388,459 bp
  • C to G, chromosome 5 at 93,273,343 bp
  • A to G, chromosome 5 at 103,527,095 bp
  • A to C, chromosome 5 at 120,802,142 bp
  • C to T, chromosome 5 at 128,788,371 bp
  • T to A, chromosome 5 at 145,699,740 bp
  • T to A, chromosome 5 at 147,349,667 bp
  • T to A, chromosome 6 at 85,341,556 bp
  • A to G, chromosome 6 at 86,628,948 bp
  • C to A, chromosome 6 at 114,245,683 bp
  • C to T, chromosome 6 at 118,594,020 bp
  • T to A, chromosome 7 at 49,552,829 bp
  • A to T, chromosome 7 at 99,140,913 bp
  • T to C, chromosome 7 at 102,542,254 bp
  • A to G, chromosome 7 at 135,699,351 bp
  • A to T, chromosome 7 at 144,508,210 bp
  • C to T, chromosome 8 at 22,014,684 bp
  • T to C, chromosome 8 at 40,912,649 bp
  • G to A, chromosome 8 at 47,864,575 bp
  • A to T, chromosome 8 at 71,352,188 bp
  • GCCCC to GCCC, chromosome 8 at 91,033,790 bp
  • T to A, chromosome 8 at 91,033,818 bp
  • T to C, chromosome 8 at 94,676,243 bp
  • A to T, chromosome 8 at 104,213,996 bp
  • G to A, chromosome 9 at 39,702,127 bp
  • G to T, chromosome 9 at 49,471,879 bp
  • A to T, chromosome 9 at 64,235,731 bp
  • C to A, chromosome 9 at 65,588,348 bp
  • T to A, chromosome 9 at 109,732,623 bp
  • T to A, chromosome 9 at 121,729,656 bp
  • T to A, chromosome 10 at 79,891,528 bp
  • T to A, chromosome 10 at 82,108,742 bp
  • A to T, chromosome 11 at 33,284,114 bp
  • A to G, chromosome 11 at 58,581,690 bp
  • C to A, chromosome 11 at 69,142,920 bp
  • C to A, chromosome 11 at 89,015,737 bp
  • A to G, chromosome 11 at 94,429,075 bp
  • A to T, chromosome 11 at 101,991,457 bp
  • A to G, chromosome 11 at 115,812,155 bp
  • T to G, chromosome 12 at 11,457,981 bp
  • T to A, chromosome 12 at 64,996,975 bp
  • T to G, chromosome 12 at 102,000,792 bp
  • T to C, chromosome 13 at 15,726,291 bp
  • A to G, chromosome 13 at 19,077,368 bp
  • G to A, chromosome 14 at 30,816,811 bp
  • A to G, chromosome 14 at 34,195,805 bp
  • A to T, chromosome 14 at 53,806,639 bp
  • C to T, chromosome 14 at 59,791,181 bp
  • C to T, chromosome 15 at 8,145,607 bp
  • A to T, chromosome 15 at 27,747,913 bp
  • A to T, chromosome 15 at 92,770,067 bp
  • T to A, chromosome 16 at 20,375,070 bp
  • T to A, chromosome 17 at 6,029,791 bp
  • C to A, chromosome 17 at 37,446,305 bp
  • A to T, chromosome 17 at 73,574,809 bp
  • A to T, chromosome 17 at 80,247,113 bp
  • A to G, chromosome 18 at 37,716,004 bp
  • G to T, chromosome 18 at 42,202,550 bp
  • T to C, chromosome 18 at 75,519,803 bp
  • C to T, chromosome 19 at 4,625,053 bp
  • A to G, chromosome 19 at 10,253,355 bp
  • G to A, chromosome X at 37,518,992 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7451 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045525-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.