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Strain Name:
C57BL/6J-MtgxR7470Btlr/Mmmh
Stock Number:
045544-MU
Citation ID:
RRID:MMRRC_045544-MU
Other Names:
R7470 (G1)
Major Collection:

Strain Information

Nfatc2
Name: nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 2
Synonyms: NFAT1, NFATp, NFAT1-D
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18019
HGNC: HGNC:7776
Homologene: 7861
Slc12a1
Name: solute carrier family 12, member 1
Synonyms: Nkcc2, mBSC1, D630042G03Rik, urehr3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20495
Homologene: 286
Vcp
Name: valosin containing protein
Synonyms: CDC48, p97, p97/VCP, AAA ATPase p97
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 269523
Homologene: 5168
Hgf
Name: hepatocyte growth factor
Synonyms: scatter factor, NK1, SF/HGF, HGF/SF, NK2, C230052L06Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 15234
HGNC: HGNC:4893
Homologene: 503
Cacna1g
Name: calcium channel, voltage-dependent, T type, alpha 1G subunit
Synonyms: a1G, Cav3.1d
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 12291
HGNC: HGNC:1394
Homologene: 22544
Spen
Name: spen family transcription repressor
Synonyms: Mint
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 56381
Homologene: 124461
Arhgef12
Name: Rho guanine nucleotide exchange factor 12
Synonyms: LARG, 2310014B11Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 69632
VEGA: 9
Homologene: 9088
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 64,042,313 bp
  • T to A, chromosome 1 at 66,622,462 bp
  • T to A, chromosome 1 at 93,008,663 bp
  • G to C, chromosome 1 at 131,764,043 bp
  • A to C, chromosome 1 at 153,754,031 bp
  • T to A, chromosome 1 at 156,376,516 bp
  • G to T, chromosome 1 at 184,928,044 bp
  • T to A, chromosome 2 at 5,138,304 bp
  • A to C, chromosome 2 at 25,909,833 bp
  • A to T, chromosome 2 at 53,042,365 bp
  • A to T, chromosome 2 at 87,962,449 bp
  • C to T, chromosome 2 at 103,734,881 bp
  • G to A, chromosome 2 at 125,217,895 bp
  • A to C, chromosome 2 at 131,075,824 bp
  • A to G, chromosome 2 at 146,424,667 bp
  • T to C, chromosome 2 at 152,687,662 bp
  • G to A, chromosome 2 at 168,523,307 bp
  • A to T, chromosome 2 at 168,925,811 bp
  • T to G, chromosome 3 at 100,963,162 bp
  • T to C, chromosome 3 at 101,451,075 bp
  • A to G, chromosome 3 at 129,855,087 bp
  • A to T, chromosome 3 at 135,242,155 bp
  • A to G, chromosome 4 at 42,982,891 bp
  • C to A, chromosome 4 at 45,990,144 bp
  • T to C, chromosome 4 at 96,535,471 bp
  • T to A, chromosome 4 at 113,756,931 bp
  • G to T, chromosome 4 at 113,885,803 bp
  • A to C, chromosome 4 at 134,539,750 bp
  • T to C, chromosome 4 at 141,479,294 bp
  • A to T, chromosome 4 at 148,261,070 bp
  • T to A, chromosome 5 at 12,508,185 bp
  • C to A, chromosome 5 at 16,618,856 bp
  • G to T, chromosome 5 at 21,942,741 bp
  • T to G, chromosome 5 at 87,254,112 bp
  • T to A, chromosome 5 at 90,531,627 bp
  • T to C, chromosome 5 at 113,942,427 bp
  • T to C, chromosome 5 at 142,992,725 bp
  • C to A, chromosome 6 at 30,900,046 bp
  • A to T, chromosome 6 at 78,376,088 bp
  • A to G, chromosome 6 at 111,501,515 bp
  • T to A, chromosome 7 at 43,047,886 bp
  • A to G, chromosome 7 at 127,475,825 bp
  • A to G, chromosome 8 at 25,735,060 bp
  • T to C, chromosome 8 at 79,673,360 bp
  • A to G, chromosome 8 at 109,638,376 bp
  • T to A, chromosome 9 at 37,919,296 bp
  • C to A, chromosome 9 at 39,613,702 bp
  • A to T, chromosome 9 at 43,040,552 bp
  • A to T, chromosome 9 at 55,991,338 bp
  • T to C, chromosome 9 at 56,620,624 bp
  • A to G, chromosome 9 at 107,005,445 bp
  • T to C, chromosome 10 at 14,444,066 bp
  • T to C, chromosome 10 at 21,358,784 bp
  • C to A, chromosome 10 at 76,508,397 bp
  • T to C, chromosome 10 at 82,059,939 bp
  • G to A, chromosome 10 at 87,563,424 bp
  • T to A, chromosome 11 at 73,395,888 bp
  • T to C, chromosome 11 at 86,776,702 bp
  • T to A, chromosome 11 at 94,461,939 bp
  • C to T, chromosome 11 at 99,145,557 bp
  • A to C, chromosome 11 at 99,804,364 bp
  • C to T, chromosome 12 at 103,094,976 bp
  • T to A, chromosome 13 at 32,816,327 bp
  • T to A, chromosome 13 at 33,486,634 bp
  • T to C, chromosome 13 at 41,163,104 bp
  • A to T, chromosome 13 at 65,292,146 bp
  • G to T, chromosome 14 at 20,309,723 bp
  • A to G, chromosome 14 at 26,427,420 bp
  • C to A, chromosome 14 at 29,998,033 bp
  • A to G, chromosome 14 at 55,066,750 bp
  • A to G, chromosome 14 at 60,802,895 bp
  • A to G, chromosome 14 at 64,028,566 bp
  • C to A, chromosome 14 at 101,900,604 bp
  • T to C, chromosome 14 at 123,572,044 bp
  • T to A, chromosome 15 at 77,717,943 bp
  • A to G, chromosome 16 at 17,575,113 bp
  • T to A, chromosome 16 at 49,884,222 bp
  • A to G, chromosome 17 at 34,899,396 bp
  • A to T, chromosome 17 at 54,276,962 bp
  • T to C, chromosome 17 at 84,226,041 bp
  • T to C, chromosome 17 at 88,562,221 bp
  • C to T, chromosome 18 at 24,108,838 bp
  • G to A, chromosome 19 at 4,867,814 bp
  • T to A, chromosome 19 at 11,076,350 bp
  • A to G, chromosome 19 at 58,732,025 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7470 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045544-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.