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Strain Name:
C57BL/6J-MtgxR7488Btlr/Mmmh
Stock Number:
045562-MU
Citation ID:
RRID:MMRRC_045562-MU
Other Names:
R7488 (G1)
Major Collection:

Strain Information

Cdk4
Name: cyclin dependent kinase 4
Synonyms: p34PSK-J3/cdk4, Crk3
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 12567
HGNC: HGNC:1773
Homologene: 55429
Tfdp2
Name: transcription factor Dp 2
Synonyms: DP3, DP-3, DP3, A330080J22Rik, 1110029I05Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 211586
Homologene: 4578
Pds5b
Name: PDS5 cohesin associated factor B
Synonyms: AS3, Aprin
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100710
Homologene: 41001
Por
Name: cytochrome p450 oxidoreductase
Synonyms: NADH cytochrome P450 oxydoreductase, CYPOR, CPR, 4933424M13Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18984
HGNC: HGNC:9208
Homologene: 725
Jak2
Name: Janus kinase 2
Synonyms: C81284
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 16452
VEGA: 19
HGNC: HGNC:6192
Homologene: 21033
Wwp1
Name: WW domain containing E3 ubiquitin protein ligase 1
Synonyms: SDRP1, Tiul1, AIP5, 8030445B08Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 107568
Homologene: 21385
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 26,683,958 bp
  • C to T, chromosome 1 at 33,779,140 bp
  • T to C, chromosome 1 at 38,050,306 bp
  • T to A, chromosome 1 at 69,539,385 bp
  • A to T, chromosome 1 at 174,837,119 bp
  • G to A, chromosome 2 at 31,420,830 bp
  • A to T, chromosome 2 at 35,296,421 bp
  • A to T, chromosome 2 at 52,220,221 bp
  • C to T, chromosome 2 at 66,757,230 bp
  • C to T, chromosome 2 at 69,277,802 bp
  • T to C, chromosome 2 at 87,358,253 bp
  • A to G, chromosome 2 at 120,039,957 bp
  • T to C, chromosome 2 at 156,077,937 bp
  • A to G, chromosome 3 at 20,270,324 bp
  • T to C, chromosome 3 at 96,560,223 bp
  • G to T, chromosome 3 at 130,584,701 bp
  • A to C, chromosome 3 at 131,297,947 bp
  • G to T, chromosome 4 at 19,627,660 bp
  • T to A, chromosome 4 at 32,602,058 bp
  • A to G, chromosome 4 at 83,473,038 bp
  • T to C, chromosome 4 at 108,266,458 bp
  • T to A, chromosome 4 at 149,982,636 bp
  • T to C, chromosome 4 at 154,032,967 bp
  • A to G, chromosome 5 at 86,326,450 bp
  • G to T, chromosome 5 at 120,624,724 bp
  • C to G, chromosome 5 at 121,208,528 bp
  • A to T, chromosome 5 at 135,733,644 bp
  • T to A, chromosome 5 at 143,991,583 bp
  • A to T, chromosome 5 at 150,723,337 bp
  • T to A, chromosome 6 at 53,947,742 bp
  • T to A, chromosome 6 at 91,265,863 bp
  • G to T, chromosome 6 at 122,581,562 bp
  • T to A, chromosome 6 at 124,779,112 bp
  • A to T, chromosome 7 at 5,610,768 bp
  • T to C, chromosome 7 at 15,989,442 bp
  • A to G, chromosome 7 at 98,615,555 bp
  • T to A, chromosome 7 at 121,763,434 bp
  • C to A, chromosome 8 at 18,933,592 bp
  • A to G, chromosome 8 at 45,962,294 bp
  • A to G, chromosome 8 at 85,055,034 bp
  • A to G, chromosome 9 at 6,359,739 bp
  • A to G, chromosome 9 at 7,124,855 bp
  • A to T, chromosome 9 at 9,970,565 bp
  • A to G, chromosome 9 at 36,954,831 bp
  • A to G, chromosome 9 at 37,730,687 bp
  • A to G, chromosome 9 at 45,175,555 bp
  • C to A, chromosome 9 at 51,290,060 bp
  • A to G, chromosome 9 at 64,924,482 bp
  • A to G, chromosome 9 at 96,297,642 bp
  • T to A, chromosome 9 at 105,402,705 bp
  • A to T, chromosome 10 at 3,957,491 bp
  • A to C, chromosome 10 at 75,160,330 bp
  • A to G, chromosome 10 at 91,054,380 bp
  • T to A, chromosome 10 at 127,064,237 bp
  • G to T, chromosome 10 at 129,111,736 bp
  • T to C, chromosome 11 at 62,589,750 bp
  • G to A, chromosome 11 at 72,759,943 bp
  • C to T, chromosome 11 at 73,238,529 bp
  • G to A, chromosome 11 at 101,200,900 bp
  • T to A, chromosome 12 at 71,150,405 bp
  • C to T, chromosome 12 at 73,982,511 bp
  • C to A, chromosome 12 at 100,857,051 bp
  • A to G, chromosome 12 at 112,785,021 bp
  • T to C, chromosome 12 at 114,048,358 bp
  • T to C, chromosome 13 at 54,717,915 bp
  • T to A, chromosome 13 at 91,675,090 bp
  • T to A, chromosome 14 at 34,567,445 bp
  • A to C, chromosome 14 at 52,638,515 bp
  • T to C, chromosome 14 at 54,632,180 bp
  • A to G, chromosome 14 at 54,933,002 bp
  • G to T, chromosome 15 at 25,889,542 bp
  • A to T, chromosome 15 at 64,120,125 bp
  • G to A, chromosome 16 at 14,389,899 bp
  • C to A, chromosome 16 at 18,540,470 bp
  • A to G, chromosome 17 at 20,208,783 bp
  • G to A, chromosome 18 at 10,122,762 bp
  • T to C, chromosome 18 at 34,824,881 bp
  • A to G, chromosome 18 at 57,191,115 bp
  • G to A, chromosome 19 at 5,667,459 bp
  • T to C, chromosome 19 at 22,978,573 bp
  • C to T, chromosome 19 at 29,298,383 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7488 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045562-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.