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Strain Name:
C57BL/6J-MtgxR7498Btlr/Mmmh
Stock Number:
045571-MU
Citation ID:
RRID:MMRRC_045571-MU
Other Names:
R7498 (G1)
Major Collection:

Strain Information

Zmpste24
Name: zinc metallopeptidase, STE24
Synonyms: A530043O15Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230709
Homologene: 4277
Tal1
Name: T cell acute lymphocytic leukemia 1
Synonyms: SCL/tal-1, Scl, bHLHa17, Hpt
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 21349
Homologene: 2400
Nudt2
Name: nudix hydrolase 2
Synonyms: APAH1, 2310051L06Rik, nudix (nucleoside diphosphate linked moiety X)-type motif 2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 66401
HGNC: HGNC:8049
Homologene: 896
Gli2
Name: GLI-Kruppel family member GLI2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14633
HGNC: HGNC:4318
Homologene: 12725
Wnk1
Name: WNK lysine deficient protein kinase 1
Synonyms: 6430573H23Rik, Prkwnk1, EG406236, Hsn2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232341
Homologene: 14253
Hdlbp
Name: high density lipoprotein (HDL) binding protein
Synonyms: D1Ertd101e, 1110005P14Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 110611
HGNC: HGNC:4857
Homologene: 38035
Rapgef6
Name: Rap guanine nucleotide exchange factor (GEF) 6
Synonyms: PDZ-GEF2, RA-GEF-2, Pdzgef2, C030018K18Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 192786
Homologene: 22968
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 9,919,077 bp
  • T to G, chromosome 1 at 46,325,765 bp
  • T to A, chromosome 1 at 93,413,615 bp
  • T to A, chromosome 1 at 95,591,693 bp
  • T to C, chromosome 1 at 118,835,835 bp
  • C to T, chromosome 1 at 119,417,878 bp
  • G to A, chromosome 2 at 24,406,984 bp
  • A to G, chromosome 2 at 25,195,792 bp
  • A to C, chromosome 2 at 31,383,475 bp
  • A to T, chromosome 2 at 32,371,749 bp
  • C to T, chromosome 2 at 52,258,176 bp
  • G to A, chromosome 2 at 68,667,668 bp
  • A to C, chromosome 2 at 70,965,625 bp
  • G to A, chromosome 2 at 90,436,565 bp
  • T to A, chromosome 2 at 135,262,233 bp
  • G to T, chromosome 2 at 135,262,234 bp
  • C to T, chromosome 3 at 127,679,778 bp
  • T to A, chromosome 3 at 148,859,216 bp
  • C to A, chromosome 4 at 15,266,176 bp
  • T to C, chromosome 4 at 41,480,539 bp
  • T to C, chromosome 4 at 45,062,194 bp
  • A to G, chromosome 4 at 99,748,696 bp
  • A to T, chromosome 4 at 115,068,682 bp
  • A to G, chromosome 4 at 121,082,831 bp
  • C to T, chromosome 4 at 155,694,738 bp
  • C to A, chromosome 5 at 26,261,190 bp
  • GGACCTCTCCGTG to GG, chromosome 5 at 33,735,422 bp
  • C to T, chromosome 5 at 87,474,641 bp
  • T to A, chromosome 5 at 97,088,119 bp
  • T to C, chromosome 5 at 118,002,174 bp
  • G to A, chromosome 5 at 135,363,914 bp
  • G to T, chromosome 6 at 83,103,231 bp
  • T to C, chromosome 6 at 119,927,196 bp
  • G to A, chromosome 7 at 29,717,102 bp
  • A to T, chromosome 7 at 64,208,909 bp
  • C to T, chromosome 7 at 80,313,150 bp
  • A to G, chromosome 7 at 96,848,017 bp
  • A to T, chromosome 7 at 106,944,368 bp
  • T to A, chromosome 7 at 108,630,416 bp
  • T to A, chromosome 8 at 10,400,589 bp
  • A to C, chromosome 8 at 69,519,823 bp
  • G to A, chromosome 8 at 84,101,763 bp
  • A to G, chromosome 8 at 123,447,844 bp
  • A to G, chromosome 9 at 22,002,257 bp
  • A to C, chromosome 9 at 51,948,598 bp
  • A to T, chromosome 9 at 86,494,411 bp
  • T to A, chromosome 10 at 81,268,901 bp
  • T to A, chromosome 10 at 82,291,279 bp
  • G to T, chromosome 11 at 54,620,004 bp
  • G to A, chromosome 11 at 59,082,713 bp
  • A to G, chromosome 11 at 67,097,048 bp
  • C to A, chromosome 11 at 67,283,437 bp
  • G to A, chromosome 11 at 74,929,106 bp
  • G to A, chromosome 11 at 78,053,362 bp
  • A to T, chromosome 11 at 82,982,187 bp
  • C to T, chromosome 12 at 53,142,705 bp
  • T to A, chromosome 12 at 65,099,391 bp
  • A to T, chromosome 12 at 77,412,934 bp
  • A to T, chromosome 13 at 55,346,977 bp
  • A to T, chromosome 13 at 81,440,225 bp
  • C to T, chromosome 14 at 54,165,361 bp
  • G to A, chromosome 14 at 103,655,993 bp
  • A to T, chromosome 15 at 4,763,364 bp
  • T to A, chromosome 15 at 9,727,539 bp
  • T to A, chromosome 15 at 81,639,843 bp
  • G to T, chromosome 15 at 85,543,679 bp
  • T to C, chromosome 16 at 5,077,105 bp
  • G to A, chromosome 17 at 33,137,859 bp
  • A to G, chromosome 17 at 33,883,872 bp
  • A to T, chromosome 17 at 37,012,092 bp
  • A to G, chromosome 17 at 37,752,351 bp
  • A to G, chromosome 17 at 40,225,802 bp
  • A to G, chromosome 17 at 74,660,470 bp
  • C to T, chromosome 18 at 89,769,319 bp
  • A to T, chromosome 19 at 9,012,019 bp
  • A to T, chromosome 19 at 18,876,120 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7498 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045571-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.