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Strain Name:
C57BL/6J-MtgxR7499Btlr/Mmmh
Stock Number:
045572-MU
Citation ID:
RRID:MMRRC_045572-MU
Other Names:
R7499 (G1)
Major Collection:

Strain Information

Dlx5
Name: distal-less homeobox 5
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 13395
HGNC: HGNC:2918
Homologene: 3825
Slco1a5
Name: solute carrier organic anion transporter family, member 1a5
Synonyms: Oatp3, Slc21a7
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 108096
Homologene: 56603
Cep170
Name: centrosomal protein 170
Synonyms: 4933426L22Rik, A330004A13Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 545389
Homologene: 22844
Emsy
Name: EMSY, BRCA2-interacting transcriptional repressor
Synonyms: 2210018M11Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233545
Homologene: 32465
Tep1
Name: telomerase associated protein 1
Synonyms: Tp1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 21745
VEGA: 14
Homologene: 5157
Clpb
Name: ClpB caseinolytic peptidase B
Synonyms: Skd3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20480
Homologene: 32067
Asb6
Name: ankyrin repeat and SOCS box-containing 6
Synonyms: 2510004M11Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 72323
Homologene: 9895
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 106,872,389 bp
  • T to A, chromosome 1 at 173,482,041 bp
  • A to T, chromosome 1 at 176,774,462 bp
  • T to C, chromosome 2 at 5,877,914 bp
  • C to T, chromosome 2 at 19,285,979 bp
  • T to C, chromosome 2 at 30,824,460 bp
  • T to C, chromosome 2 at 174,644,023 bp
  • T to C, chromosome 3 at 28,630,594 bp
  • G to A, chromosome 3 at 73,701,898 bp
  • A to G, chromosome 3 at 90,225,533 bp
  • A to T, chromosome 3 at 96,579,821 bp
  • A to G, chromosome 3 at 123,374,854 bp
  • T to C, chromosome 4 at 46,539,188 bp
  • T to C, chromosome 4 at 83,005,770 bp
  • T to C, chromosome 4 at 88,522,674 bp
  • T to A, chromosome 4 at 137,405,639 bp
  • T to A, chromosome 4 at 139,306,512 bp
  • C to A, chromosome 5 at 26,261,190 bp
  • GGACCTCTCCGTG to GG, chromosome 5 at 33,735,422 bp
  • T to C, chromosome 5 at 110,151,683 bp
  • T to C, chromosome 5 at 121,818,473 bp
  • G to A, chromosome 5 at 129,873,268 bp
  • C to A, chromosome 5 at 137,032,245 bp
  • G to A, chromosome 5 at 137,464,356 bp
  • G to A, chromosome 5 at 137,747,723 bp
  • G to A, chromosome 6 at 6,878,340 bp
  • A to C, chromosome 6 at 6,878,341 bp
  • A to G, chromosome 6 at 30,630,857 bp
  • C to A, chromosome 6 at 125,144,208 bp
  • T to C, chromosome 6 at 142,262,531 bp
  • A to G, chromosome 7 at 39,409,065 bp
  • T to C, chromosome 7 at 83,674,494 bp
  • A to G, chromosome 7 at 98,630,331 bp
  • T to C, chromosome 7 at 101,722,728 bp
  • A to G, chromosome 7 at 108,171,800 bp
  • A to G, chromosome 7 at 120,060,912 bp
  • A to T, chromosome 7 at 142,383,412 bp
  • T to C, chromosome 8 at 12,832,575 bp
  • T to C, chromosome 8 at 115,693,181 bp
  • A to G, chromosome 8 at 121,883,722 bp
  • T to A, chromosome 9 at 55,999,902 bp
  • A to G, chromosome 9 at 56,488,943 bp
  • A to T, chromosome 10 at 33,196,101 bp
  • T to C, chromosome 10 at 127,022,548 bp
  • T to A, chromosome 11 at 51,591,458 bp
  • T to C, chromosome 11 at 62,879,463 bp
  • G to A, chromosome 11 at 89,391,750 bp
  • C to A, chromosome 11 at 101,376,694 bp
  • A to G, chromosome 13 at 24,693,772 bp
  • G to A, chromosome 14 at 31,315,122 bp
  • G to A, chromosome 14 at 50,853,590 bp
  • G to A, chromosome 14 at 62,665,027 bp
  • A to G, chromosome 14 at 64,896,677 bp
  • A to T, chromosome 15 at 28,302,450 bp
  • G to T, chromosome 15 at 79,602,420 bp
  • G to A, chromosome 15 at 83,800,050 bp
  • T to C, chromosome 16 at 8,603,754 bp
  • G to A, chromosome 17 at 35,144,392 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7499 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045572-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.