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Strain Name:
C57BL/6J-MtgxR7509Btlr/Mmmh
Stock Number:
045582-MU
Citation ID:
RRID:MMRRC_045582-MU
Other Names:
R7509 (G1)
Major Collection:

Strain Information

Tnpo1
Name: transportin 1
Synonyms: D13Ertd688e, Kpnb2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 238799
HGNC: HGNC:6401
Homologene: 5358
Erbb4
Name: erb-b2 receptor tyrosine kinase 4
Synonyms: ErbB4, Her4
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13869
HGNC: HGNC:3432
Homologene: 21084
Pola2
Name: polymerase (DNA directed), alpha 2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 18969
VEGA: 19
Homologene: 48121
Med13l
Name: mediator complex subunit 13-like
Synonyms: 2210413I17Rik, 6330591G05Rik, Trap240L, 9030618F05Rik, Thrap2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 76199
Homologene: 25256
Trpm7
Name: transient receptor potential cation channel, subfamily M, member 7
Synonyms: 5033407O22Rik, 4833414K03Rik, Ltpr7, CHAK1, CHAK, TRP-PLIK, 2310022G15Rik, LTRPC7
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 58800
Homologene: 9774
Suco
Name: SUN domain containing ossification factor
Synonyms: osteopotentia, Opt, AI848100
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226551
HGNC: HGNC:1240
Homologene: 32212
Helb
Name: helicase (DNA) B
Synonyms: D10Ertd664e
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 117599
Homologene: 50463
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 34,500,199 bp
  • T to C, chromosome 1 at 37,387,830 bp
  • A to T, chromosome 1 at 68,250,580 bp
  • A to G, chromosome 1 at 83,026,260 bp
  • A to T, chromosome 1 at 161,845,334 bp
  • A to T, chromosome 1 at 161,970,976 bp
  • A to G, chromosome 1 at 178,077,512 bp
  • A to T, chromosome 2 at 126,849,922 bp
  • A to T, chromosome 2 at 150,577,704 bp
  • A to T, chromosome 2 at 161,013,154 bp
  • C to T, chromosome 2 at 177,945,700 bp
  • A to T, chromosome 3 at 100,151,187 bp
  • T to C, chromosome 3 at 105,032,730 bp
  • A to T, chromosome 4 at 58,090,683 bp
  • G to A, chromosome 4 at 101,020,651 bp
  • A to C, chromosome 4 at 134,252,113 bp
  • G to A, chromosome 5 at 45,696,108 bp
  • T to A, chromosome 5 at 57,720,187 bp
  • A to T, chromosome 5 at 109,427,829 bp
  • A to T, chromosome 5 at 110,330,705 bp
  • A to G, chromosome 5 at 118,748,930 bp
  • C to T, chromosome 5 at 142,718,506 bp
  • A to G, chromosome 5 at 151,647,510 bp
  • T to C, chromosome 6 at 81,953,275 bp
  • AC to ACC, chromosome 6 at 113,690,898 bp
  • C to T, chromosome 6 at 123,385,423 bp
  • T to C, chromosome 6 at 125,642,169 bp
  • A to G, chromosome 7 at 81,038,340 bp
  • T to C, chromosome 7 at 105,406,890 bp
  • A to G, chromosome 7 at 119,534,388 bp
  • A to T, chromosome 7 at 141,892,141 bp
  • A to G, chromosome 8 at 25,955,726 bp
  • T to G, chromosome 8 at 111,259,043 bp
  • T to A, chromosome 9 at 38,349,572 bp
  • T to A, chromosome 9 at 39,623,327 bp
  • T to A, chromosome 9 at 44,336,911 bp
  • C to T, chromosome 9 at 62,781,940 bp
  • T to G, chromosome 9 at 77,181,396 bp
  • T to A, chromosome 9 at 82,025,577 bp
  • G to A, chromosome 10 at 79,396,008 bp
  • G to T, chromosome 10 at 86,713,432 bp
  • A to G, chromosome 10 at 93,710,568 bp
  • A to T, chromosome 10 at 100,466,094 bp
  • A to G, chromosome 10 at 117,659,840 bp
  • T to A, chromosome 10 at 120,089,814 bp
  • G to A, chromosome 10 at 123,032,552 bp
  • T to A, chromosome 11 at 30,998,507 bp
  • G to A, chromosome 11 at 59,051,629 bp
  • C to T, chromosome 11 at 67,210,461 bp
  • C to T, chromosome 11 at 76,797,876 bp
  • C to A, chromosome 11 at 106,187,094 bp
  • T to C, chromosome 12 at 24,982,361 bp
  • T to C, chromosome 12 at 116,365,876 bp
  • G to T, chromosome 13 at 70,787,164 bp
  • T to A, chromosome 13 at 96,947,170 bp
  • A to C, chromosome 13 at 98,870,243 bp
  • G to A, chromosome 14 at 30,895,447 bp
  • A to G, chromosome 14 at 36,938,645 bp
  • G to A, chromosome 14 at 70,266,682 bp
  • A to G, chromosome 14 at 70,548,488 bp
  • G to A, chromosome 14 at 74,947,608 bp
  • G to A, chromosome 14 at 77,797,024 bp
  • T to C, chromosome 15 at 74,958,286 bp
  • G to A, chromosome 15 at 99,722,086 bp
  • T to A, chromosome 16 at 4,939,218 bp
  • C to A, chromosome 16 at 37,060,343 bp
  • T to A, chromosome 16 at 37,060,344 bp
  • A to T, chromosome 17 at 7,928,736 bp
  • G to A, chromosome 17 at 18,582,733 bp
  • C to G, chromosome 17 at 26,438,746 bp
  • C to A, chromosome 18 at 37,342,021 bp
  • T to A, chromosome 18 at 37,675,857 bp
  • A to G, chromosome 18 at 45,683,120 bp
  • A to T, chromosome 18 at 50,164,682 bp
  • G to T, chromosome 18 at 60,603,494 bp
  • A to G, chromosome 18 at 89,247,071 bp
  • A to T, chromosome 19 at 5,961,166 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7509 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045582-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.