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Strain Name:
C57BL/6J-MtgxR7542Btlr/Mmmh
Stock Number:
045614-MU
Citation ID:
RRID:MMRRC_045614-MU
Other Names:
R7542 (G1)
Major Collection:

Strain Information

Frem2
Name: Fras1 related extracellular matrix protein 2
Synonyms: 8430406N05Rik, 6030440P17Rik, my, ne, b2b1562Clo
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 242022
Homologene: 18454
Manba
Name: mannosidase, beta A, lysosomal
Synonyms: Bmn, 2410030O07Rik, B930014J03Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 110173
HGNC: HGNC:6831
Homologene: 4317
Tmem59l
Name: transmembrane protein 59-like
Synonyms: 5330410G16Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 67937
Homologene: 8104
Odf2l
Name: outer dense fiber of sperm tails 2-like
Synonyms: 4733401D09Rik, 9630045K08Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 52184
Homologene: 12011
Fam53a
Name: family with sequence similarity 53, member A
Synonyms: 5430419M09Rik, DNTNP, 2410018C17Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 74504
Homologene: 18660
Urb2
Name: URB2 ribosome biogenesis 2 homolog (S. cerevisiae)
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 382038
Homologene: 8859
Med25
Name: mediator complex subunit 25
Synonyms: ESTM2, 2610529E18Rik, 2610034E13Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 75613
Homologene: 12614
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 46,784,498 bp
  • C to T, chromosome 1 at 131,711,641 bp
  • A to T, chromosome 2 at 82,984,852 bp
  • A to T, chromosome 2 at 88,958,775 bp
  • C to T, chromosome 2 at 118,121,174 bp
  • A to G, chromosome 2 at 118,791,118 bp
  • A to G, chromosome 3 at 38,981,355 bp
  • A to T, chromosome 3 at 38,981,621 bp
  • A to T, chromosome 3 at 53,652,579 bp
  • A to G, chromosome 3 at 54,315,654 bp
  • A to G, chromosome 3 at 83,269,284 bp
  • A to G, chromosome 3 at 97,766,655 bp
  • A to T, chromosome 3 at 108,458,227 bp
  • T to C, chromosome 3 at 135,566,593 bp
  • A to C, chromosome 3 at 145,153,436 bp
  • A to G, chromosome 4 at 3,595,439 bp
  • G to T, chromosome 4 at 43,445,941 bp
  • C to A, chromosome 5 at 21,955,181 bp
  • A to T, chromosome 5 at 30,933,593 bp
  • T to C, chromosome 5 at 33,371,663 bp
  • A to G, chromosome 5 at 33,607,471 bp
  • A to T, chromosome 5 at 123,148,447 bp
  • T to C, chromosome 5 at 137,819,523 bp
  • A to T, chromosome 6 at 85,629,362 bp
  • T to C, chromosome 7 at 8,255,489 bp
  • A to T, chromosome 7 at 33,173,322 bp
  • A to G, chromosome 7 at 44,595,714 bp
  • A to T, chromosome 7 at 44,891,791 bp
  • G to A, chromosome 7 at 45,020,342 bp
  • A to T, chromosome 7 at 46,376,673 bp
  • A to G, chromosome 7 at 80,393,459 bp
  • A to T, chromosome 7 at 104,382,218 bp
  • C to T, chromosome 8 at 18,631,689 bp
  • T to C, chromosome 8 at 21,843,344 bp
  • A to T, chromosome 8 at 40,680,809 bp
  • T to C, chromosome 8 at 70,485,164 bp
  • A to G, chromosome 8 at 70,708,766 bp
  • A to T, chromosome 8 at 83,395,545 bp
  • A to G, chromosome 8 at 124,028,588 bp
  • A to T, chromosome 8 at 124,675,103 bp
  • C to A, chromosome 9 at 26,818,195 bp
  • T to A, chromosome 9 at 39,594,631 bp
  • T to C, chromosome 9 at 40,211,147 bp
  • C to A, chromosome 9 at 57,629,000 bp
  • T to A, chromosome 9 at 73,012,459 bp
  • T to A, chromosome 9 at 89,601,911 bp
  • A to T, chromosome 9 at 106,651,169 bp
  • A to G, chromosome 10 at 81,514,302 bp
  • A to T, chromosome 10 at 109,823,533 bp
  • T to C, chromosome 10 at 128,018,034 bp
  • T to C, chromosome 11 at 62,850,596 bp
  • T to C, chromosome 11 at 70,321,756 bp
  • T to C, chromosome 11 at 80,478,364 bp
  • T to A, chromosome 11 at 102,400,045 bp
  • A to G, chromosome 11 at 106,014,561 bp
  • A to G, chromosome 12 at 53,069,234 bp
  • T to C, chromosome 12 at 72,506,320 bp
  • A to G, chromosome 13 at 34,638,163 bp
  • A to T, chromosome 14 at 42,466,784 bp
  • G to A, chromosome 14 at 50,862,491 bp
  • A to T, chromosome 14 at 78,510,292 bp
  • T to A, chromosome 15 at 25,941,210 bp
  • C to T, chromosome 17 at 15,370,347 bp
  • T to C, chromosome 17 at 28,363,755 bp
  • G to A, chromosome 18 at 36,940,089 bp
  • G to A, chromosome 18 at 36,947,731 bp
  • C to A, chromosome 18 at 57,189,570 bp
  • G to A, chromosome 19 at 4,333,830 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7542 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045614-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.