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Strain Name:
C57BL/6J-MtgxR7563Btlr/Mmmh
Stock Number:
045655-MU
Citation ID:
RRID:MMRRC_045655-MU
Other Names:
R7563 (G1)
Major Collection:

Strain Information

Kmt2e
Name: lysine (K)-specific methyltransferase 2E
Synonyms: 1810033J14Rik, D230038D11Rik, 9530077A04Rik, Mll5
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 69188
Homologene: 18822
Ssc4d
Name: scavenger receptor cysteine rich family, 4 domains
Synonyms: C330016E03Rik, Srcrb4d
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 109267
Homologene: 34137
Top2a
Name: topoisomerase (DNA) II alpha
Synonyms: DNA Topoisomerase II alpha, Top-2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 21973
Homologene: 830
Ipo5
Name: importin 5
Synonyms: RanBP5, 5730478E03Rik, 1110011C18Rik, Kpnb3, IMB3, importin beta 3
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 70572
VEGA: 14
HGNC: HGNC:6402
Homologene: 1710
Epha8
Name: Eph receptor A8
Synonyms: EphA8, Hek3, Eek
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 13842
HGNC: HGNC:3391
Homologene: 22436
Trim39
Name: tripartite motif-containing 39
Synonyms: RING-B box-coiled-coil-B30.2, RBCC-B30.2, tfp, 1100001D15Rik, Rnf23, E130103K13Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 79263
Homologene: 10940
Slc30a5
Name: solute carrier family 30 (zinc transporter), member 5
Synonyms: Zntl1, ZnT-5, ZTL1, 1810010K08Rik, Znt5
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 69048
VEGA: 13
Homologene: 41503
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 58,047,145 bp
  • T to C, chromosome 1 at 154,471,416 bp
  • T to C, chromosome 2 at 67,509,901 bp
  • T to A, chromosome 2 at 85,887,138 bp
  • T to C, chromosome 2 at 111,306,789 bp
  • T to C, chromosome 2 at 165,716,130 bp
  • T to A, chromosome 2 at 180,592,507 bp
  • C to T, chromosome 3 at 32,954,476 bp
  • A to G, chromosome 3 at 64,275,349 bp
  • T to C, chromosome 3 at 107,214,232 bp
  • A to G, chromosome 4 at 21,732,220 bp
  • A to C, chromosome 4 at 41,569,208 bp
  • T to C, chromosome 4 at 136,938,789 bp
  • A to T, chromosome 4 at 143,654,105 bp
  • A to G, chromosome 4 at 144,457,894 bp
  • T to C, chromosome 5 at 23,500,273 bp
  • C to T, chromosome 5 at 43,821,549 bp
  • T to C, chromosome 5 at 53,185,984 bp
  • T to C, chromosome 5 at 120,788,956 bp
  • A to G, chromosome 5 at 135,963,033 bp
  • G to A, chromosome 5 at 139,544,506 bp
  • G to T, chromosome 6 at 70,171,903 bp
  • TG to TGG, chromosome 6 at 108,664,857 bp
  • A to G, chromosome 6 at 129,593,738 bp
  • T to A, chromosome 7 at 5,488,201 bp
  • C to A, chromosome 7 at 25,081,352 bp
  • A to T, chromosome 7 at 89,509,830 bp
  • G to T, chromosome 7 at 100,608,197 bp
  • T to C, chromosome 8 at 46,293,593 bp
  • A to T, chromosome 8 at 66,468,313 bp
  • A to T, chromosome 8 at 83,420,556 bp
  • T to A, chromosome 8 at 93,178,039 bp
  • A to G, chromosome 8 at 105,315,756 bp
  • A to G, chromosome 8 at 109,525,374 bp
  • G to T, chromosome 9 at 77,112,997 bp
  • A to T, chromosome 9 at 90,219,010 bp
  • A to C, chromosome 9 at 90,226,248 bp
  • A to G, chromosome 9 at 108,379,344 bp
  • G to A, chromosome 10 at 43,876,517 bp
  • A to T, chromosome 10 at 82,660,825 bp
  • C to A, chromosome 11 at 95,021,413 bp
  • T to C, chromosome 11 at 99,016,179 bp
  • G to A, chromosome 11 at 100,741,854 bp
  • T to C, chromosome 12 at 64,473,285 bp
  • T to G, chromosome 13 at 27,314,238 bp
  • A to T, chromosome 13 at 100,803,972 bp
  • T to C, chromosome 13 at 109,951,007 bp
  • A to G, chromosome 14 at 55,099,749 bp
  • T to A, chromosome 14 at 120,946,155 bp
  • T to C, chromosome 16 at 34,392,094 bp
  • C to T, chromosome 17 at 23,291,026 bp
  • A to T, chromosome 17 at 23,717,328 bp
  • A to T, chromosome 17 at 36,260,915 bp
  • T to A, chromosome 17 at 45,633,965 bp
  • T to C, chromosome 19 at 9,011,165 bp
  • T to A, chromosome 19 at 23,720,316 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7563 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045655-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.