Strain Name:
C57BL/6J-MtgxR7576Btlr/Mmmh
Stock Number:
045661-MU
Citation ID:
RRID:MMRRC_045661-MU
Other Names:
R7576 (G1)
Major Collection:

Strain Information

Neurod1
Name: neurogenic differentiation 1
Synonyms: Neurod, Nd1, bHLHa3, BETA2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18012
HGNC: HGNC:7762
Homologene: 1871
Nrxn2
Name: neurexin II
Synonyms: neurexin II beta, 6430591O13Rik, neurexin II alpha
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 18190
HGNC: HGNC:8009
Homologene: 86984
Rbpms
Name: RNA binding protein gene with multiple splicing
Synonyms: 2700019M19Rik, 2010300K22Rik, hermes
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 19663
Homologene: 38238
Pds5b
Name: PDS5 cohesin associated factor B
Synonyms: Aprin, AS3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100710
Homologene: 41001
Tns3
Name: tensin 3
Synonyms: TEM6, F830010I22Rik, Tens1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 319939
Homologene: 49713
Ahdc1
Name: AT hook, DNA binding motif, containing 1
Synonyms: D030015G18Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230793
Homologene: 17144
Myh10
Name: myosin, heavy polypeptide 10, non-muscle
Synonyms: nonmuscle myosin heavy chain IIB, SMemb, NMHC-B, nonmuscle myosin heavy chain II-B, Myhn-2, 5730504C04Rik, myosin IIB, Fltn, Myhn2, Fltn, NMHC II-B, Myosin IIB, 9330167F11Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 77579
HGNC: HGNC:7568
Homologene: 55941
Ccdc91
Name: coiled-coil domain containing 91
Synonyms: 1700086G08Rik, 1810060J02Rik, p56
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 67015
Homologene: 10131
Hnrnpll
Name: heterogeneous nuclear ribonucleoprotein L-like
Synonyms: 2510028H02Rik, 2810036L13Rik, Hnrpll
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 72692
Homologene: 26701
Cdk5rap2
Name: CDK5 regulatory subunit associated protein 2
Synonyms: an, 2900018K03Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 214444
Homologene: 49533
Akap9
Name: A kinase anchor protein 9
Synonyms: G1-448-15, repro12, AKAP450, mei2-5, 5730481H23Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100986
HGNC: HGNC:379
Homologene: 134583
Ubr7
Name: ubiquitin protein ligase E3 component n-recognin 7 (putative)
Synonyms: 5730410I19Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 66622
VEGA: 12
Homologene: 11998
Epcam
Name: epithelial cell adhesion molecule
Synonyms: gp40, panepithelial glycoprotein 314, EpCAM1, Ly74, Egp314, EpCAM, Tacstd1, Ep-CAM, EGP-2, GA733-2, TROP1, CD326
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 17075
VEGA: 17
Homologene: 1764
Robo1
Name: roundabout guidance receptor 1
Synonyms: DUTT1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19876
Homologene: 2206
Bcl2
Name: B cell leukemia/lymphoma 2
Synonyms: C430015F12Rik, Bcl-2, D830018M01Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12043
HGNC: HGNC:990
Homologene: 527
Gja8
Name: gap junction protein, alpha 8
Synonyms: connexin 50, alpha 8 connexin, dcm, Lop10, Aey5, Cnx50, Cx50
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14616
HGNC: HGNC:4281
Homologene: 3857
Erc1
Name: ELKS/RAB6-interacting/CAST family member 1
Synonyms: RAB6IP2B, RAB6IP2A, Rab6ip2, 9630025C19Rik, 5033405M01Rik, Elks1, B430107L16Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 111173
Homologene: 14229
Ppid
Name: peptidylprolyl isomerase D (cyclophilin D)
Synonyms: cytoplasmic cyclophilin D, cyclophilin 40, CYP-40, Ppidl, 4930564J03Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 67738
HGNC: HGNC:9257
Homologene: 31283
Pacs1
Name: phosphofurin acidic cluster sorting protein 1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 107975
VEGA: 19
Homologene: 9970
Zfp960
Name: zinc finger protein 960
Synonyms: BC018101
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 449000
Homologene: 133884
Steap2
Name: six transmembrane epithelial antigen of prostate 2
Synonyms: 4921538B17Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 74051
Homologene: 17682
Mansc1
Name: MANSC domain containing 1
Synonyms: 9130403P13Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 67729
Homologene: 49522
Lama1
Name: laminin, alpha 1
Synonyms: Lama
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 16772
VEGA: 17
HGNC: HGNC:6481
Homologene: 21146
Carm1
Name: coactivator-associated arginine methyltransferase 1
Synonyms: m9Bei, Prmt4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 59035
Homologene: 10990
Golm1
Name: golgi membrane protein 1
Synonyms: Golph2, GP73, PSEC0257, D030064E01Rik, 2310001L02Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 105348
VEGA: 13
Homologene: 12346
Dync2i2
Name: dynein 2 intermediate chain 2
Synonyms: Wdr34, 3200002I06Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 71820
Homologene: 14156
Tctn1
Name: tectonic family member 1
Synonyms: G730031O11Rik, Tect1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 654470
Homologene: 49770
Hectd4
Name: HECT domain E3 ubiquitin protein ligase 4
Synonyms: Gm15800
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 269700
Homologene: 28297
Ppp2r5b
Name: protein phosphatase 2, regulatory subunit B', beta
Synonyms: B'beta
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 225849
HGNC: HGNC:9310
Homologene: 38157
Slc5a2
Name: solute carrier family 5 (sodium/glucose cotransporter), member 2
Synonyms: Sglt2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 246787
Homologene: 2289
Muc4
Name: mucin 4
Synonyms: 4933405I11Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 140474
HGNC: HGNC:7514
Homologene: 124469
Enpep
Name: glutamyl aminopeptidase
Synonyms: Ly51, aminopeptidase-A, 6030431M22Rik, APA, Bp-1/6C3, Ly-51
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 13809
HGNC: HGNC:3355
Homologene: 68216
Glt6d1
Name: glycosyltransferase 6 domain containing 1
Synonyms: 4933411C14Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 71103
Homologene: 18826
Brinp3
Name: bone morphogenetic protein/retinoic acid inducible neural specific 3
Synonyms: Fam5c, B830045N13Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 215378
Homologene: 17786
Mast3
Name: microtubule associated serine/threonine kinase 3
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 546071
Homologene: 66191
Ptprt
Name: protein tyrosine phosphatase receptor type T
Synonyms: RPTPrho
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 19281
HGNC: HGNC:9682
Homologene: 56924
Fndc8
Name: fibronectin type III domain containing 8
Synonyms: 4930466G16Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 78919
Homologene: 9716
Pigp
Name: phosphatidylinositol glycan anchor biosynthesis, class P
Synonyms: Dcrc, Dscr5
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 56176
HGNC: HGNC:3046
Homologene: 32444
Ciao3
Name: cytosolic iron-sulfur assembly component 3
Synonyms: 9030612I22Rik, Narfl
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 67563
Homologene: 6750
Alpk2
Name: alpha-kinase 2
Synonyms: Hak
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225638
Homologene: 50475
Rfx4
Name: regulatory factor X, 4 (influences HLA class II expression)
Synonyms: 4933412G19Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 71137
HGNC: HGNC:9985
Homologene: 31119
Ssc5d
Name: scavenger receptor cysteine rich family, 5 domains
Synonyms: A430110N23Rik, s5d-srcrb
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 269855
Homologene: 77555
Nwd2
Name: NACHT and WD repeat domain containing 2
Synonyms: 3110047P20Rik, B830017A01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 319807
Homologene: 14974
Ppp4r4
Name: protein phosphatase 4, regulatory subunit 4
Synonyms: 8430415E04Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 74521
Homologene: 12571
Ppef2
Name: protein phosphatase, EF hand calcium-binding domain 2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 19023
HGNC: HGNC:9244
Homologene: 55959
Vmn2r92
Name: vomeronasal 2, receptor 92
Synonyms: EG627111
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 627111
Homologene: 115024
Ccdc30
Name: coiled-coil domain containing 30
Synonyms: 4930445I03Rik, 1700001O02Rik, 1700111D19Rik, 1700041C02Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 73332
Homologene: 137391
Retreg2
Name: reticulophagy regulator family member 2
Synonyms: MGC47289, Fam134a
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 227298
Homologene: 11507
Gemin6
Name: gem nuclear organelle associated protein 6
Synonyms: 2610019B15Rik, 2810470M17Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 67242
Homologene: 11711
Or10j3b
Name: olfactory receptor family 10 subfamily J member 3B
Synonyms: MOR267-2, GA_x6K02T2R7CC-630397-629456, Olfr1404
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 258881
Homologene: 133587
Zfp229
Name: zinc finger protein 229
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 381067
Homologene: 136287
Zc3h6
Name: zinc finger CCCH type containing 6
Synonyms: 4631426G04Rik, 4833425H18Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 78751
Homologene: 35328
Fmn1
Name: formin 1
Synonyms: Fmn, formin-1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14260
HGNC: HGNC:3768
Homologene: 121778
Gbp10
Name: guanylate-binding protein 10
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 626578
Homologene: 128731
Usp16
Name: ubiquitin specific peptidase 16
Synonyms: 1200004E02Rik, UBP-M, 2810483I07Rik, 6330514E22Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 74112
Homologene: 38183
Ilvbl
Name: ilvB (bacterial acetolactate synthase)-like
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216136
VEGA: 10
HGNC: HGNC:6041
Homologene: 68532
Sac3d1
Name: SAC3 domain containing 1
Synonyms: Shd1, 2410004C24Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 66406
VEGA: 19
Homologene: 40867
Usp44
Name: ubiquitin specific peptidase 44
Synonyms: E430004F17Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 327799
VEGA: 10
Homologene: 12961
Smyd4
Name: SET and MYND domain containing 4
Synonyms: G430029E23Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 319822
Homologene: 35098
Calr4
Name: calreticulin 4
Synonyms: 4933403L16Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 108802
Homologene: 86813
Lin7b
Name: lin-7 homolog B, crumbs cell polarity complex component
Synonyms: MALS-2, Veli2, LIN-7B
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22342
Homologene: 22648
Fastkd1
Name: FAST kinase domains 1
Synonyms: 5330408N05Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 320720
Homologene: 36420
Gje1
Name: gap junction protein, epsilon 1
Synonyms: Gjf1, AEY12, D230044M03Rik, Gsfaey12, connexin 23, Cx23
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 76743
Homologene: 87405
Gltpd2
Name: glycolipid transfer protein domain containing 2
Synonyms: C730027E14Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216871
Homologene: 51691
She
Name: src homology 2 domain-containing transforming protein E
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 214547
Homologene: 18186
Or6z7
Name: olfactory receptor family 6 subfamily Z member 7
Synonyms: GA_x6K02T2QGBW-3210997-3210059, Olfr5, MOR103-8
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18349
Homologene: 72037
Gm10153
Name: predicted gene 10153
Type: Gene
Species: Mouse
Chromosome: 7
Tmtc2
Name: transmembrane and tetratricopeptide repeat containing 2
Synonyms: D330034A10Rik, 8430438D04Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 278279
Homologene: 25521
Krtap5-20
Name: keratin associated protein 5-20
Synonyms: Gm4559
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 100043627
VEGA: 7
Or8u3-ps
Name: olfactory receptor family 8 subfamily U member 3
Synonyms: Olfr1038-ps, GA_x6K02T2Q125-47591072-47592031, MOR185-3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 259015
Homologene: 17463
Ighv7-3
Name: immunoglobulin heavy variable 7-3
Synonyms: Gm16842
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 629822
Igkv8-24
Name: immunoglobulin kappa chain variable 8-24
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 677858
HGNC: HGNC:5834
Scgb2b19
Name: secretoglobin, family 2B, member 19
Synonyms: Gm5894, Abpbg19
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 545947
Homologene: 83171
Gm28729
Name: predicted gene 28729
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 102635744
Homologene: 139355
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 75,144,688 bp
  • A to G, chromosome 1 at 106,712,423 bp
  • A to T, chromosome 1 at 146,901,563 bp
  • A to G, chromosome 1 at 173,215,971 bp
  • A to G, chromosome 2 at 25,814,122 bp
  • C to T, chromosome 2 at 30,048,778 bp
  • A to G, chromosome 2 at 69,694,644 bp
  • G to T, chromosome 2 at 79,454,345 bp
  • A to G, chromosome 2 at 86,122,376 bp
  • A to G, chromosome 2 at 113,365,008 bp
  • C to T, chromosome 2 at 129,014,553 bp
  • T to C, chromosome 2 at 161,607,305 bp
  • T to A, chromosome 3 at 79,600,391 bp
  • T to C, chromosome 3 at 89,831,612 bp
  • T to C, chromosome 3 at 96,919,893 bp
  • T to A, chromosome 3 at 129,284,091 bp
  • T to C, chromosome 4 at 70,266,872 bp
  • A to T, chromosome 4 at 109,238,964 bp
  • A to G, chromosome 4 at 119,349,866 bp
  • A to T, chromosome 4 at 133,065,002 bp
  • C to G, chromosome 5 at 3,968,745 bp
  • A to G, chromosome 5 at 5,677,406 bp
  • A to G, chromosome 5 at 63,807,393 bp
  • T to A, chromosome 5 at 92,253,134 bp
  • G to A, chromosome 5 at 105,236,149 bp
  • A to T, chromosome 5 at 121,349,459 bp
  • A to T, chromosome 5 at 122,248,008 bp
  • A to G, chromosome 5 at 150,778,261 bp
  • A to G, chromosome 6 at 70,217,018 bp
  • G to T, chromosome 6 at 119,824,760 bp
  • T to A, chromosome 6 at 134,610,711 bp
  • C to G, chromosome 6 at 147,590,459 bp
  • C to A, chromosome 7 at 4,928,573 bp
  • T to A, chromosome 7 at 6,480,331 bp
  • A to T, chromosome 7 at 33,279,786 bp
  • A to T, chromosome 7 at 45,369,225 bp
  • G to A, chromosome 7 at 128,265,805 bp
  • A to C, chromosome 7 at 142,190,122 bp
  • A to G, chromosome 7 at 142,273,940 bp
  • A to G, chromosome 8 at 33,866,388 bp
  • A to G, chromosome 8 at 70,781,194 bp
  • T to G, chromosome 9 at 21,586,536 bp
  • G to A, chromosome 9 at 96,494,357 bp
  • A to G, chromosome 10 at 14,716,757 bp
  • A to T, chromosome 10 at 78,583,697 bp
  • A to G, chromosome 10 at 84,863,349 bp
  • A to G, chromosome 10 at 93,846,428 bp
  • A to G, chromosome 10 at 105,370,621 bp
  • T to A, chromosome 11 at 8,541,192 bp
  • A to G, chromosome 11 at 68,802,166 bp
  • T to A, chromosome 11 at 70,519,480 bp
  • T to A, chromosome 11 at 75,390,206 bp
  • T to G, chromosome 11 at 82,897,574 bp
  • T to G, chromosome 12 at 102,769,139 bp
  • A to G, chromosome 12 at 103,596,449 bp
  • A to T, chromosome 12 at 114,153,245 bp
  • T to C, chromosome 13 at 59,645,106 bp
  • C to T, chromosome 15 at 76,187,746 bp
  • C to T, chromosome 16 at 32,754,500 bp
  • C to T, chromosome 16 at 72,970,181 bp
  • T to C, chromosome 16 at 87,479,300 bp
  • A to G, chromosome 16 at 94,370,405 bp
  • A to G, chromosome 17 at 17,087,965 bp
  • A to C, chromosome 17 at 18,167,359 bp
  • A to G, chromosome 17 at 21,745,299 bp
  • A to C, chromosome 17 at 25,778,970 bp
  • G to A, chromosome 17 at 67,782,041 bp
  • G to A, chromosome 17 at 80,044,514 bp
  • T to A, chromosome 17 at 80,225,726 bp
  • T to C, chromosome 17 at 87,640,293 bp
  • T to C, chromosome 18 at 65,306,816 bp
  • T to A, chromosome 19 at 5,145,120 bp
  • T to A, chromosome 19 at 6,116,148 bp
  • T to G, chromosome 19 at 6,228,484 bp
  • G to T, chromosome 19 at 6,531,510 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7576 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045661-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.