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Strain Name:
C57BL/6J-MtgxR7708Btlr/Mmmh
Stock Number:
045709-MU
Citation ID:
RRID:MMRRC_045709-MU
Other Names:
R7708 (G1)
Major Collection:

Strain Information

Tnf
Name: tumor necrosis factor
Synonyms: TNF-alpha, tumor necrosis factor-alpha, TNF alpha, TNFalpha, DIF, Tnfsf1a, Tnfa
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 21926
Homologene: 496
Scn3a
Name: sodium channel, voltage-gated, type III, alpha
Synonyms: LOC381367, Nav1.3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20269
Homologene: 56005
Mtmr7
Name: myotubularin related protein 7
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 54384
HGNC: HGNC:7454
Homologene: 99732
Phf21a
Name: PHD finger protein 21A
Synonyms: 80kDa, Braf35/HDAC complex (Bhc), PFTF1, Bhc80, D030065N23Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 192285
Homologene: 9597
Fkbp5
Name: FK506 binding protein 5
Synonyms: FKBP51, Dit1, D17Ertd592e
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 14229
HGNC: HGNC:3721
Homologene: 3038
Asap1
Name: ArfGAP with SH3 domain, ankyrin repeat and PH domain1
Synonyms: Ddef1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 13196
HGNC: HGNC:2720
Homologene: 7684
Clock
Name: clock circadian regulator
Synonyms: 5330400M04Rik, KAT13D, bHLHe8
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 12753
HGNC: HGNC:2082
Homologene: 3603
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 132,325,032 bp
  • A to G, chromosome 1 at 174,117,734 bp
  • A to G, chromosome 1 at 187,232,766 bp
  • C to T, chromosome 2 at 65,483,168 bp
  • T to C, chromosome 2 at 92,327,166 bp
  • T to A, chromosome 2 at 111,144,617 bp
  • T to C, chromosome 2 at 111,231,518 bp
  • A to G, chromosome 3 at 59,865,335 bp
  • G to A, chromosome 3 at 96,880,625 bp
  • A to T, chromosome 3 at 126,597,440 bp
  • G to T, chromosome 4 at 62,496,100 bp
  • A to T, chromosome 4 at 63,357,813 bp
  • T to A, chromosome 5 at 76,266,409 bp
  • C to T, chromosome 5 at 86,524,169 bp
  • C to T, chromosome 5 at 103,629,262 bp
  • T to A, chromosome 5 at 123,997,469 bp
  • T to G, chromosome 6 at 41,535,683 bp
  • A to G, chromosome 6 at 52,164,562 bp
  • G to A, chromosome 7 at 24,916,881 bp
  • T to A, chromosome 7 at 85,412,547 bp
  • T to C, chromosome 7 at 103,310,561 bp
  • C to T, chromosome 7 at 108,630,841 bp
  • A to G, chromosome 8 at 40,590,511 bp
  • A to G, chromosome 8 at 40,680,519 bp
  • T to C, chromosome 8 at 79,175,962 bp
  • A to T, chromosome 9 at 38,473,385 bp
  • A to T, chromosome 9 at 39,593,755 bp
  • A to G, chromosome 10 at 116,162,597 bp
  • C to A, chromosome 11 at 43,202,143 bp
  • G to C, chromosome 11 at 104,964,571 bp
  • C to T, chromosome 12 at 30,006,602 bp
  • T to G, chromosome 12 at 72,069,959 bp
  • T to C, chromosome 12 at 112,607,557 bp
  • T to C, chromosome 13 at 23,114,099 bp
  • A to G, chromosome 13 at 37,929,570 bp
  • T to A, chromosome 13 at 42,164,277 bp
  • T to A, chromosome 13 at 63,841,514 bp
  • C to T, chromosome 13 at 104,147,337 bp
  • T to C, chromosome 15 at 64,152,872 bp
  • A to T, chromosome 15 at 98,313,148 bp
  • T to C, chromosome 15 at 101,487,932 bp
  • A to G, chromosome 17 at 28,438,097 bp
  • A to C, chromosome 17 at 35,200,158 bp
  • G to T, chromosome 17 at 46,172,700 bp
  • A to G, chromosome 17 at 46,779,345 bp
  • T to A, chromosome 18 at 37,671,443 bp
  • G to T, chromosome 18 at 74,216,243 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7708 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045709-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.