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Strain Name:
C57BL/6J-MtgxR7644Btlr/Mmmh
Stock Number:
045723-MU
Citation ID:
RRID:MMRRC_045723-MU
Other Names:
R7644 (G1)
Major Collection:

Strain Information

Smarca4
Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Synonyms: SNF2beta, Brg1, SW1/SNF, b2b692Clo, b2b508.1Clo
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20586
Homologene: 135927
Clasp1
Name: CLIP associating protein 1
Synonyms: 5730583A19Rik, 1700030C23Rik, mCLASP1, CLASP1alpha, CLASP1, B130045P17Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 76707
Homologene: 41024
Dennd2b
Name: DENN domain containing 2B
Synonyms: 2610305K15Rik, 2010004M01Rik, St5, Denn2b
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 76954
Homologene: 3951
Abl2
Name: ABL proto-oncogene 2, non-receptor tyrosine kinase
Synonyms: Arg, Abll
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 11352
HGNC: HGNC:77
Homologene: 5278
Adgrl2
Name: adhesion G protein-coupled receptor L2
Synonyms: Lphh1, Lec1, Lphn2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 99633
Homologene: 22712
Sf3b1
Name: splicing factor 3b, subunit 1
Synonyms: SAP155, Prp10, SF3b155, 2810001M05Rik, Targ4
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 81898
Homologene: 6696
Srrm2
Name: serine/arginine repetitive matrix 2
Synonyms: 5033413A03Rik, SRm300
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 75956
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 24,185,162 bp
  • G to A, chromosome 1 at 54,997,143 bp
  • T to C, chromosome 1 at 63,058,771 bp
  • T to C, chromosome 1 at 88,200,785 bp
  • T to G, chromosome 1 at 118,512,750 bp
  • T to C, chromosome 1 at 133,914,618 bp
  • G to T, chromosome 1 at 135,256,506 bp
  • G to A, chromosome 1 at 138,067,907 bp
  • A to G, chromosome 1 at 156,615,993 bp
  • A to G, chromosome 1 at 178,679,274 bp
  • C to T, chromosome 2 at 30,797,954 bp
  • T to A, chromosome 2 at 69,696,840 bp
  • T to A, chromosome 2 at 71,410,181 bp
  • T to C, chromosome 2 at 76,919,780 bp
  • A to T, chromosome 2 at 91,041,954 bp
  • T to A, chromosome 2 at 160,896,770 bp
  • A to G, chromosome 3 at 39,010,241 bp
  • C to T, chromosome 3 at 89,745,519 bp
  • G to T, chromosome 3 at 108,413,490 bp
  • C to T, chromosome 3 at 148,839,153 bp
  • A to G, chromosome 3 at 152,357,952 bp
  • T to A, chromosome 4 at 63,395,397 bp
  • A to G, chromosome 4 at 135,733,035 bp
  • A to T, chromosome 4 at 138,317,372 bp
  • T to C, chromosome 5 at 21,978,931 bp
  • A to G, chromosome 5 at 107,620,301 bp
  • G to C, chromosome 5 at 109,339,971 bp
  • A to G, chromosome 6 at 34,872,773 bp
  • T to C, chromosome 6 at 41,690,808 bp
  • G to A, chromosome 6 at 72,309,835 bp
  • C to T, chromosome 6 at 123,125,015 bp
  • G to A, chromosome 7 at 14,561,691 bp
  • G to A, chromosome 7 at 15,957,549 bp
  • A to C, chromosome 7 at 43,769,710 bp
  • G to A, chromosome 7 at 44,191,880 bp
  • C to A, chromosome 7 at 79,444,471 bp
  • A to T, chromosome 7 at 79,451,668 bp
  • A to G, chromosome 7 at 85,957,538 bp
  • A to G, chromosome 7 at 92,567,660 bp
  • A to G, chromosome 7 at 108,565,442 bp
  • A to T, chromosome 7 at 109,556,793 bp
  • G to A, chromosome 7 at 127,032,962 bp
  • G to C, chromosome 7 at 141,637,746 bp
  • A to G, chromosome 8 at 22,640,427 bp
  • T to C, chromosome 8 at 26,013,488 bp
  • T to A, chromosome 8 at 33,574,417 bp
  • C to T, chromosome 8 at 69,887,894 bp
  • C to T, chromosome 8 at 71,484,830 bp
  • C to A, chromosome 8 at 71,634,538 bp
  • T to A, chromosome 8 at 94,658,768 bp
  • T to C, chromosome 8 at 117,801,884 bp
  • T to A, chromosome 8 at 122,357,322 bp
  • A to T, chromosome 9 at 14,734,415 bp
  • G to T, chromosome 9 at 21,655,654 bp
  • C to A, chromosome 9 at 39,047,342 bp
  • T to C, chromosome 9 at 101,936,194 bp
  • T to A, chromosome 9 at 102,537,773 bp
  • T to C, chromosome 10 at 14,760,689 bp
  • T to A, chromosome 10 at 116,048,228 bp
  • C to T, chromosome 10 at 117,770,470 bp
  • A to G, chromosome 10 at 128,953,501 bp
  • C to A, chromosome 11 at 8,875,758 bp
  • T to C, chromosome 11 at 69,400,206 bp
  • C to T, chromosome 11 at 70,345,542 bp
  • T to C, chromosome 11 at 95,750,088 bp
  • T to A, chromosome 11 at 99,908,222 bp
  • A to G, chromosome 12 at 98,855,944 bp
  • T to A, chromosome 13 at 25,107,691 bp
  • T to A, chromosome 13 at 41,030,110 bp
  • G to T, chromosome 13 at 61,356,968 bp
  • G to A, chromosome 13 at 67,541,449 bp
  • C to T, chromosome 13 at 100,678,376 bp
  • T to C, chromosome 14 at 33,944,890 bp
  • T to A, chromosome 14 at 56,167,319 bp
  • A to G, chromosome 14 at 66,297,097 bp
  • T to A, chromosome 15 at 8,223,127 bp
  • C to T, chromosome 15 at 64,699,369 bp
  • T to C, chromosome 15 at 92,310,009 bp
  • G to A, chromosome 15 at 99,594,226 bp
  • T to C, chromosome 16 at 14,221,824 bp
  • A to T, chromosome 17 at 23,477,291 bp
  • G to A, chromosome 17 at 23,819,320 bp
  • A to T, chromosome 17 at 33,950,445 bp
  • T to G, chromosome 18 at 49,893,552 bp
  • G to A, chromosome 18 at 76,944,175 bp
  • C to T, chromosome 19 at 5,450,304 bp
  • T to C, chromosome 19 at 32,811,834 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7644 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045723-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.