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Strain Name:
C57BL/6J-MtgxR7651Btlr/Mmmh
Stock Number:
045728-MU
Citation ID:
RRID:MMRRC_045728-MU
Other Names:
R7651 (G1)
Major Collection:

Strain Information

Hyal1
Name: hyaluronoglucosaminidase 1
Synonyms: Hyal-1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 15586
HGNC: HGNC:5320
Homologene: 5277
Gnb5
Name: guanine nucleotide binding protein (G protein), beta 5
Synonyms: G beta 5, Gbeta5
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 14697
VEGA: 9
HGNC: HGNC:4401
Homologene: 40714
Tnfsf4
Name: tumor necrosis factor (ligand) superfamily, member 4
Synonyms: OX40L, gp34, Txgp1l, Ath-1, TXGP1, CD134L, Ath1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 22164
Homologene: 2495
Utp20
Name: UTP20 small subunit processome component
Synonyms: mDRIM, DRIM, 3830408P06Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 70683
VEGA: 10
Homologene: 38373
Fgr
Name: FGR proto-oncogene, Src family tyrosine kinase
Synonyms: Ali18, Mhdaali18
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 14191
HGNC: HGNC:3697
Homologene: 3842
Ap2b1
Name: adaptor-related protein complex 2, beta 1 subunit
Synonyms: 1300012O03Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 71770
HGNC: HGNC:563
Homologene: 137384
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 105,809,446 bp
  • T to A, chromosome 1 at 140,570,461 bp
  • T to A, chromosome 1 at 150,454,945 bp
  • T to C, chromosome 1 at 161,417,022 bp
  • T to A, chromosome 2 at 163,732,355 bp
  • T to C, chromosome 2 at 167,188,361 bp
  • T to C, chromosome 3 at 14,481,449 bp
  • C to A, chromosome 3 at 86,741,466 bp
  • T to C, chromosome 4 at 94,582,639 bp
  • T to C, chromosome 4 at 94,923,707 bp
  • A to T, chromosome 4 at 132,995,013 bp
  • C to T, chromosome 4 at 134,251,648 bp
  • A to G, chromosome 4 at 135,690,608 bp
  • A to T, chromosome 4 at 139,669,627 bp
  • A to T, chromosome 4 at 154,028,099 bp
  • A to G, chromosome 4 at 154,291,067 bp
  • A to G, chromosome 5 at 30,359,614 bp
  • A to G, chromosome 5 at 36,027,978 bp
  • A to G, chromosome 5 at 37,134,273 bp
  • G to C, chromosome 5 at 90,467,355 bp
  • T to C, chromosome 5 at 94,303,377 bp
  • A to T, chromosome 5 at 114,733,235 bp
  • A to G, chromosome 5 at 118,056,601 bp
  • G to A, chromosome 5 at 122,367,382 bp
  • A to C, chromosome 5 at 147,354,922 bp
  • A to T, chromosome 6 at 27,760,258 bp
  • A to G, chromosome 6 at 29,444,050 bp
  • A to G, chromosome 6 at 52,172,273 bp
  • C to T, chromosome 6 at 139,622,072 bp
  • A to G, chromosome 7 at 46,241,761 bp
  • T to A, chromosome 7 at 85,751,938 bp
  • A to G, chromosome 7 at 127,774,064 bp
  • A to T, chromosome 7 at 130,623,154 bp
  • T to C, chromosome 7 at 141,192,151 bp
  • A to T, chromosome 7 at 141,704,201 bp
  • A to G, chromosome 7 at 141,796,254 bp
  • A to G, chromosome 7 at 141,864,023 bp
  • C to T, chromosome 7 at 142,273,816 bp
  • T to C, chromosome 8 at 12,279,689 bp
  • C to A, chromosome 8 at 12,396,686 bp
  • G to T, chromosome 8 at 25,017,300 bp
  • T to C, chromosome 8 at 94,037,353 bp
  • T to C, chromosome 9 at 20,588,512 bp
  • T to C, chromosome 9 at 45,773,852 bp
  • T to C, chromosome 9 at 75,343,571 bp
  • T to A, chromosome 9 at 75,682,050 bp
  • T to C, chromosome 9 at 80,264,266 bp
  • T to C, chromosome 9 at 83,548,472 bp
  • T to C, chromosome 9 at 89,729,410 bp
  • G to A, chromosome 9 at 107,578,370 bp
  • T to A, chromosome 9 at 109,494,646 bp
  • T to A, chromosome 10 at 5,205,074 bp
  • A to T, chromosome 10 at 5,343,416 bp
  • C to T, chromosome 10 at 21,156,374 bp
  • T to C, chromosome 10 at 88,754,595 bp
  • C to T, chromosome 10 at 99,016,968 bp
  • T to C, chromosome 10 at 116,251,179 bp
  • T to A, chromosome 11 at 30,837,334 bp
  • T to G, chromosome 11 at 58,293,362 bp
  • T to C, chromosome 11 at 83,339,430 bp
  • A to T, chromosome 11 at 83,645,085 bp
  • C to T, chromosome 11 at 95,001,942 bp
  • T to A, chromosome 12 at 105,176,435 bp
  • C to A, chromosome 12 at 115,567,385 bp
  • G to T, chromosome 13 at 62,518,513 bp
  • A to T, chromosome 14 at 31,528,335 bp
  • T to A, chromosome 14 at 53,337,507 bp
  • T to A, chromosome 14 at 88,469,153 bp
  • A to T, chromosome 15 at 10,328,378 bp
  • G to A, chromosome 15 at 12,859,436 bp
  • T to A, chromosome 16 at 32,756,575 bp
  • A to G, chromosome 17 at 20,502,349 bp
  • T to C, chromosome 17 at 22,559,051 bp
  • G to T, chromosome 17 at 23,291,012 bp
  • A to T, chromosome 17 at 29,148,205 bp
  • T to C, chromosome 18 at 37,490,993 bp
  • C to T, chromosome 18 at 77,981,400 bp
  • C to T, chromosome 18 at 82,554,374 bp
  • C to T, chromosome 19 at 17,120,408 bp
  • C to A, chromosome 19 at 60,467,145 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7651 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045728-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.