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Strain Name:
C57BL/6J-MtgxR7662Btlr/Mmmh
Stock Number:
045737-MU
Citation ID:
RRID:MMRRC_045737-MU
Other Names:
R7662 (G1)
Major Collection:

Strain Information

Dspp
Name: dentin sialophosphoprotein
Synonyms: Dmp3, Dpp, Dsp
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 666279
HGNC: HGNC:3054
Med1
Name: mediator complex subunit 1
Synonyms: TRAP 220, TRAP220, CRSP210, DRIP205, Pparbp, l11Jus15, PBP
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 19014
HGNC: HGNC:9234
Homologene: 21002
Arid5b
Name: AT-rich interaction domain 5B
Synonyms: 5430435G07Rik, Desrt, Mrf2beta, Mrf2alpha, Mrf2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 71371
VEGA: 10
Homologene: 45872
Cilk1
Name: ciliogenesis associated kinase 1
Synonyms: 2210420N10Rik, Ick
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 56542
Homologene: 69218
Lrp5
Name: low density lipoprotein receptor-related protein 5
Synonyms: LRP7, LR3
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 16973
HGNC: HGNC:6697
Homologene: 1746
Cdc6
Name: cell division cycle 6
Synonyms: cell division cycle 18 homolog (S.pombe)-like, CDC18L, CDC18(S.pombe)
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 23834
HGNC: HGNC:1744
Homologene: 68172
Cep290
Name: centrosomal protein 290
Synonyms: Nphp6, b2b1454Clo, b2b1752Clo, Kiaa
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216274
VEGA: 10
Homologene: 77213
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 23,877,774 bp
  • T to C, chromosome 1 at 93,940,892 bp
  • T to A, chromosome 1 at 118,199,407 bp
  • T to C, chromosome 1 at 150,382,294 bp
  • T to C, chromosome 1 at 153,825,479 bp
  • C to A, chromosome 2 at 6,553,917 bp
  • T to A, chromosome 2 at 15,871,454 bp
  • T to C, chromosome 2 at 31,382,345 bp
  • A to G, chromosome 2 at 76,862,400 bp
  • G to A, chromosome 2 at 174,646,141 bp
  • T to A, chromosome 3 at 27,131,798 bp
  • T to A, chromosome 3 at 30,643,303 bp
  • T to A, chromosome 3 at 89,403,451 bp
  • A to G, chromosome 3 at 132,177,868 bp
  • A to T, chromosome 3 at 132,674,066 bp
  • G to A, chromosome 4 at 32,818,694 bp
  • A to T, chromosome 4 at 58,957,679 bp
  • A to T, chromosome 4 at 111,877,540 bp
  • C to A, chromosome 4 at 117,213,741 bp
  • T to A, chromosome 4 at 118,726,307 bp
  • T to C, chromosome 4 at 139,010,561 bp
  • T to C, chromosome 4 at 140,728,995 bp
  • T to C, chromosome 4 at 152,104,298 bp
  • T to C, chromosome 5 at 8,969,072 bp
  • A to T, chromosome 5 at 104,177,870 bp
  • A to G, chromosome 5 at 120,662,184 bp
  • G to A, chromosome 5 at 144,357,890 bp
  • A to G, chromosome 5 at 144,832,511 bp
  • T to A, chromosome 6 at 3,562,304 bp
  • T to A, chromosome 6 at 24,566,090 bp
  • A to G, chromosome 6 at 39,493,103 bp
  • A to T, chromosome 6 at 77,636,869 bp
  • G to A, chromosome 6 at 92,049,931 bp
  • A to G, chromosome 6 at 123,331,562 bp
  • T to A, chromosome 7 at 21,360,627 bp
  • A to G, chromosome 7 at 29,953,853 bp
  • A to T, chromosome 7 at 35,787,804 bp
  • A to T, chromosome 7 at 43,189,912 bp
  • T to C, chromosome 7 at 55,979,624 bp
  • T to A, chromosome 7 at 86,811,284 bp
  • T to C, chromosome 7 at 140,294,812 bp
  • C to T, chromosome 7 at 141,078,379 bp
  • A to G, chromosome 8 at 48,335,727 bp
  • A to C, chromosome 8 at 85,539,276 bp
  • T to A, chromosome 8 at 114,282,344 bp
  • C to T, chromosome 8 at 125,818,771 bp
  • A to T, chromosome 9 at 9,661,385 bp
  • C to A, chromosome 9 at 36,870,500 bp
  • A to T, chromosome 9 at 39,394,093 bp
  • G to A, chromosome 9 at 55,978,715 bp
  • A to G, chromosome 9 at 64,852,431 bp
  • G to C, chromosome 9 at 78,167,620 bp
  • C to T, chromosome 9 at 79,775,231 bp
  • A to G, chromosome 9 at 87,027,038 bp
  • A to G, chromosome 9 at 120,494,211 bp
  • C to T, chromosome 10 at 68,098,587 bp
  • T to C, chromosome 10 at 76,387,522 bp
  • C to A, chromosome 10 at 77,846,852 bp
  • T to A, chromosome 10 at 100,537,803 bp
  • A to T, chromosome 11 at 57,507,565 bp
  • T to C, chromosome 11 at 58,447,443 bp
  • T to C, chromosome 11 at 98,155,392 bp
  • T to A, chromosome 11 at 98,910,612 bp
  • T to C, chromosome 11 at 98,917,010 bp
  • T to A, chromosome 11 at 108,942,456 bp
  • A to G, chromosome 12 at 29,826,869 bp
  • T to A, chromosome 12 at 112,639,738 bp
  • T to A, chromosome 13 at 21,192,158 bp
  • A to T, chromosome 13 at 54,463,804 bp
  • T to A, chromosome 14 at 30,917,330 bp
  • C to A, chromosome 14 at 41,134,212 bp
  • T to C, chromosome 14 at 44,561,933 bp
  • T to A, chromosome 15 at 55,076,165 bp
  • C to A, chromosome 15 at 57,822,176 bp
  • A to T, chromosome 15 at 103,462,585 bp
  • A to G, chromosome 16 at 56,617,323 bp
  • G to T, chromosome 17 at 18,305,369 bp
  • A to G, chromosome 17 at 46,166,435 bp
  • A to C, chromosome 17 at 78,839,055 bp
  • A to C, chromosome 18 at 13,844,116 bp
  • G to A, chromosome 18 at 37,498,735 bp
  • A to G, chromosome 19 at 3,686,353 bp
  • A to T, chromosome 19 at 18,642,178 bp
  • T to C, chromosome 19 at 47,681,501 bp
  • G to T, chromosome 19 at 56,320,283 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7662 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045737-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.