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Strain Name:
C57BL/6J-MtgxR7695Btlr/Mmmh
Stock Number:
045758-MU
Citation ID:
RRID:MMRRC_045758-MU
Other Names:
R7695 (G1)
Major Collection:

Strain Information

Myh9
Name: myosin, heavy polypeptide 9, non-muscle
Synonyms: D0Jmb2, E030044M24Rik, NMHC II-A, Myhn-1, Myhn1, myosin IIA, Fltn
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 17886
HGNC: HGNC:7579
Homologene: 129835
Ide
Name: insulin degrading enzyme
Synonyms: 1300012G03Rik, 4833415K22Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 15925
HGNC: HGNC:5381
Homologene: 3645
Pax2
Name: paired box 2
Synonyms: Pax-2, Opdc
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 18504
HGNC: HGNC:8616
Homologene: 2968
Ubap2
Name: ubiquitin-associated protein 2
Synonyms: 1190005K07Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 68926
Homologene: 73649
Dnmt1
Name: DNA methyltransferase 1
Synonyms: MTase, Dnmt1o, Cxxc9, MommeD2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 13433
VEGA: 9
HGNC: HGNC:2976
Homologene: 124071
Mfap4
Name: microfibrillar-associated protein 4
Synonyms: 1110007F23Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 76293
HGNC: HGNC:7035
Homologene: 134529
Zdbf2
Name: zinc finger, DBF-type containing 2
Synonyms: 9330107J05Rik, 4930431J08Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 73884
Homologene: 52868
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 11,162,273 bp
  • T to A, chromosome 1 at 63,307,370 bp
  • G to A, chromosome 1 at 85,579,092 bp
  • A to G, chromosome 2 at 65,711,907 bp
  • T to A, chromosome 2 at 76,835,961 bp
  • A to G, chromosome 2 at 90,118,863 bp
  • A to G, chromosome 2 at 111,645,625 bp
  • A to G, chromosome 3 at 33,814,519 bp
  • A to T, chromosome 3 at 59,326,191 bp
  • T to A, chromosome 3 at 87,739,462 bp
  • G to A, chromosome 3 at 108,402,753 bp
  • A to G, chromosome 4 at 41,211,740 bp
  • A to G, chromosome 4 at 46,875,687 bp
  • G to A, chromosome 4 at 126,180,098 bp
  • T to C, chromosome 5 at 36,947,182 bp
  • T to C, chromosome 5 at 104,435,143 bp
  • G to T, chromosome 5 at 114,256,587 bp
  • A to C, chromosome 5 at 143,085,904 bp
  • A to T, chromosome 6 at 70,687,311 bp
  • A to T, chromosome 6 at 78,377,136 bp
  • A to G, chromosome 6 at 87,384,440 bp
  • A to T, chromosome 6 at 126,534,211 bp
  • C to T, chromosome 7 at 99,336,292 bp
  • T to C, chromosome 7 at 102,723,659 bp
  • T to A, chromosome 7 at 130,728,903 bp
  • A to G, chromosome 7 at 139,086,123 bp
  • A to G, chromosome 8 at 3,926,005 bp
  • T to G, chromosome 8 at 13,798,279 bp
  • A to G, chromosome 8 at 84,920,928 bp
  • A to T, chromosome 8 at 95,770,632 bp
  • A to G, chromosome 9 at 5,353,641 bp
  • C to A, chromosome 9 at 20,913,985 bp
  • A to T, chromosome 9 at 58,902,929 bp
  • A to T, chromosome 10 at 21,299,217 bp
  • A to T, chromosome 11 at 61,485,719 bp
  • T to C, chromosome 11 at 69,969,501 bp
  • T to A, chromosome 11 at 73,940,894 bp
  • A to T, chromosome 12 at 72,541,576 bp
  • A to G, chromosome 13 at 62,456,744 bp
  • T to A, chromosome 13 at 74,363,178 bp
  • T to C, chromosome 13 at 98,984,258 bp
  • A to G, chromosome 14 at 7,551,798 bp
  • A to G, chromosome 14 at 43,725,006 bp
  • A to T, chromosome 14 at 115,092,594 bp
  • A to T, chromosome 15 at 47,820,381 bp
  • T to C, chromosome 15 at 74,543,638 bp
  • G to A, chromosome 15 at 76,183,855 bp
  • A to G, chromosome 15 at 77,766,736 bp
  • C to A, chromosome 17 at 22,455,021 bp
  • T to A, chromosome 17 at 47,597,496 bp
  • T to A, chromosome 18 at 34,428,245 bp
  • T to C, chromosome 18 at 34,794,559 bp
  • G to A, chromosome 19 at 37,329,036 bp
  • G to A, chromosome 19 at 41,928,080 bp
  • A to G, chromosome 19 at 44,833,199 bp
  • T to A, chromosome 19 at 47,267,831 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7695 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045758-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.