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Strain Name:
C57BL/6J-MtgxR7698Btlr/Mmmh
Stock Number:
045759-MU
Citation ID:
RRID:MMRRC_045759-MU
Other Names:
R7698 (G1)
Major Collection:

Strain Information

Camkk2
Name: calcium/calmodulin-dependent protein kinase kinase 2, beta
Synonyms: 6330570N16Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 207565
HGNC: HGNC:1470
Homologene: 32756
Ank2
Name: ankyrin 2, brain
Synonyms: ankyrin B, Ank-2, Ankyrin-2, Ankyrin-B, Gm4392
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 109676
HGNC: HGNC:493
Dst
Name: dystonin
Synonyms: bullous pemphigoid antigen 1, BPAG1-n, BPAG1, Bpag1, Bpag, ah, bullous pemphigoid antigen 1, athetoid, Macf2, 2310001O04Rik, nmf203, A830042E19Rik, nmf339
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Mtmr7
Name: myotubularin related protein 7
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 54384
HGNC: HGNC:7454
Homologene: 99732
Spen
Name: spen family transcription repressor
Synonyms: Mint
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 56381
Homologene: 124461
Phf20
Name: PHD finger protein 20
Synonyms: 6820402O20Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228829
Homologene: 9507
Ppp1r9a
Name: protein phosphatase 1, regulatory subunit 9A
Synonyms: 4930518N04Rik, neurabin-I, A230094E16Rik, 2810430P21Rik, Neurabin I, NRB
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 243725
Homologene: 14247
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 24,312,078 bp
  • G to A, chromosome 1 at 34,190,387 bp
  • T to G, chromosome 1 at 37,625,371 bp
  • C to A, chromosome 1 at 119,961,929 bp
  • T to C, chromosome 1 at 132,122,378 bp
  • A to T, chromosome 1 at 162,639,445 bp
  • A to T, chromosome 1 at 189,662,072 bp
  • T to C, chromosome 1 at 193,161,767 bp
  • T to C, chromosome 2 at 31,423,153 bp
  • G to T, chromosome 2 at 72,364,681 bp
  • C to G, chromosome 2 at 72,438,314 bp
  • A to T, chromosome 2 at 105,126,816 bp
  • A to T, chromosome 2 at 122,280,764 bp
  • T to A, chromosome 2 at 156,294,138 bp
  • C to T, chromosome 2 at 156,749,095 bp
  • T to C, chromosome 2 at 164,083,743 bp
  • G to A, chromosome 3 at 103,165,043 bp
  • A to G, chromosome 3 at 127,032,211 bp
  • T to C, chromosome 4 at 26,327,763 bp
  • C to T, chromosome 4 at 43,366,735 bp
  • C to T, chromosome 4 at 43,414,900 bp
  • C to G, chromosome 4 at 58,832,660 bp
  • C to T, chromosome 4 at 63,225,718 bp
  • A to G, chromosome 4 at 141,472,845 bp
  • T to A, chromosome 4 at 148,558,498 bp
  • C to T, chromosome 4 at 155,002,787 bp
  • T to A, chromosome 5 at 65,808,157 bp
  • A to G, chromosome 5 at 122,746,419 bp
  • A to T, chromosome 6 at 4,906,430 bp
  • T to C, chromosome 6 at 86,707,415 bp
  • C to A, chromosome 6 at 116,043,802 bp
  • T to A, chromosome 6 at 142,565,753 bp
  • A to T, chromosome 7 at 25,273,172 bp
  • A to T, chromosome 7 at 89,923,681 bp
  • T to A, chromosome 7 at 105,035,907 bp
  • T to C, chromosome 7 at 122,169,258 bp
  • G to T, chromosome 8 at 21,666,629 bp
  • C to T, chromosome 8 at 22,258,987 bp
  • G to A, chromosome 8 at 40,606,884 bp
  • T to C, chromosome 9 at 35,777,176 bp
  • T to A, chromosome 9 at 38,115,892 bp
  • A to T, chromosome 9 at 61,412,856 bp
  • T to C, chromosome 9 at 65,042,336 bp
  • C to T, chromosome 9 at 80,217,656 bp
  • G to A, chromosome 9 at 110,817,311 bp
  • T to C, chromosome 10 at 41,348,076 bp
  • A to T, chromosome 11 at 60,720,092 bp
  • G to A, chromosome 11 at 69,405,981 bp
  • A to T, chromosome 11 at 75,190,441 bp
  • A to G, chromosome 11 at 75,510,972 bp
  • A to G, chromosome 11 at 109,429,007 bp
  • A to G, chromosome 12 at 11,283,140 bp
  • A to T, chromosome 12 at 75,949,064 bp
  • A to G, chromosome 12 at 76,405,309 bp
  • T to C, chromosome 12 at 80,174,537 bp
  • T to C, chromosome 13 at 11,761,315 bp
  • T to C, chromosome 13 at 24,911,748 bp
  • T to A, chromosome 13 at 61,162,567 bp
  • C to A, chromosome 14 at 34,052,613 bp
  • T to C, chromosome 14 at 55,062,849 bp
  • C to A, chromosome 14 at 75,206,211 bp
  • A to T, chromosome 15 at 41,096,091 bp
  • T to C, chromosome 15 at 82,558,970 bp
  • C to A, chromosome 16 at 16,240,659 bp
  • A to G, chromosome 16 at 22,939,309 bp
  • C to G, chromosome 16 at 33,234,447 bp
  • C to T, chromosome 16 at 44,433,786 bp
  • C to A, chromosome 16 at 88,506,356 bp
  • T to C, chromosome 17 at 17,733,334 bp
  • T to C, chromosome 17 at 46,439,940 bp
  • A to G, chromosome 18 at 37,732,631 bp
  • A to G, chromosome 18 at 64,571,022 bp
  • T to C, chromosome 19 at 12,862,713 bp
  • T to C, chromosome 19 at 13,375,040 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7698 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045759-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.