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Strain Name:
C57BL/6J-MtgxR7721Btlr/Mmmh
Stock Number:
045777-MU
Citation ID:
RRID:MMRRC_045777-MU
Other Names:
R7721 (G1)
Major Collection:

Strain Information

Mob4
Name: MOB family member 4, phocein
Synonyms: 2610109B12Rik, Prei3, Mobkl3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 19070
Homologene: 9116
Dtx4
Name: deltex 4, E3 ubiquitin ligase
Synonyms: RNF155
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 207521
VEGA: 19
Homologene: 27781
Ankrd11
Name: ankyrin repeat domain 11
Synonyms: 2410104C19Rik, 9530048I21Rik, 3010027A04Rik, Yod
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 77087
Homologene: 69134
Tpr
Name: translocated promoter region, nuclear basket protein
Synonyms: 2610029M07Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 108989
Homologene: 37753
Faf1
Name: Fas-associated factor 1
Synonyms: Fam, Dffrx
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 14084
HGNC: HGNC:3578
Homologene: 5120
Uqcc1
Name: ubiquinol-cytochrome c reductase complex assembly factor 1
Synonyms: mbFZb, 3110038N19Rik, Bfzp, 2310079L17Rik, 2410003P15Rik, Uqcc, Cbp3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 56046
Homologene: 14709
Dmtf1
Name: cyclin D binding myb like transcription factor 1
Synonyms: Dmp1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 23857
Homologene: 8017
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 53,631,683 bp
  • C to T, chromosome 1 at 55,148,320 bp
  • A to G, chromosome 1 at 150,444,429 bp
  • A to G, chromosome 2 at 104,258,529 bp
  • T to C, chromosome 2 at 155,858,146 bp
  • G to A, chromosome 2 at 166,577,890 bp
  • T to G, chromosome 3 at 59,097,899 bp
  • A to T, chromosome 3 at 92,738,030 bp
  • T to A, chromosome 4 at 33,944,416 bp
  • T to C, chromosome 4 at 43,957,770 bp
  • T to A, chromosome 4 at 109,736,597 bp
  • C to A, chromosome 4 at 124,991,417 bp
  • T to C, chromosome 5 at 6,771,263 bp
  • T to C, chromosome 5 at 9,126,564 bp
  • A to G, chromosome 5 at 112,355,517 bp
  • T to C, chromosome 5 at 121,646,866 bp
  • A to G, chromosome 5 at 136,968,161 bp
  • C to T, chromosome 5 at 151,586,693 bp
  • A to G, chromosome 6 at 124,630,058 bp
  • A to G, chromosome 6 at 133,851,727 bp
  • T to G, chromosome 7 at 82,606,520 bp
  • A to G, chromosome 7 at 139,188,153 bp
  • T to G, chromosome 8 at 35,826,263 bp
  • T to C, chromosome 8 at 70,291,012 bp
  • G to T, chromosome 8 at 122,894,759 bp
  • C to T, chromosome 9 at 36,637,863 bp
  • A to T, chromosome 9 at 38,523,717 bp
  • A to G, chromosome 9 at 39,184,760 bp
  • T to C, chromosome 9 at 50,776,642 bp
  • A to T, chromosome 9 at 110,985,880 bp
  • T to A, chromosome 10 at 127,760,252 bp
  • T to A, chromosome 11 at 69,121,526 bp
  • A to T, chromosome 11 at 83,187,563 bp
  • T to A, chromosome 11 at 104,724,540 bp
  • A to G, chromosome 11 at 116,966,801 bp
  • G to T, chromosome 12 at 36,157,144 bp
  • T to A, chromosome 13 at 20,280,803 bp
  • A to C, chromosome 13 at 58,236,985 bp
  • T to A, chromosome 13 at 71,960,057 bp
  • A to G, chromosome 14 at 31,141,456 bp
  • T to A, chromosome 14 at 44,103,038 bp
  • A to T, chromosome 14 at 52,263,760 bp
  • G to T, chromosome 14 at 63,133,316 bp
  • A to T, chromosome 15 at 89,101,401 bp
  • T to A, chromosome 16 at 31,990,434 bp
  • C to A, chromosome 16 at 64,786,207 bp
  • G to T, chromosome 17 at 25,836,149 bp
  • T to A, chromosome 17 at 40,207,518 bp
  • A to G, chromosome 17 at 56,771,819 bp
  • A to T, chromosome 18 at 36,999,636 bp
  • A to G, chromosome 19 at 12,482,136 bp
  • T to A, chromosome 19 at 13,726,843 bp
  • T to A, chromosome 19 at 57,696,331 bp
  • A to T, chromosome 19 at 61,226,586 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7721 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045777-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.