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Strain Name:
C57BL/6J-MtgxR7723Btlr/Mmmh
Stock Number:
045779-MU
Citation ID:
RRID:MMRRC_045779-MU
Other Names:
R7723 (G1)
Major Collection:

Strain Information

Nts
Name: neurotensin
Synonyms: neuromedin N, NT/N, NTS1, NMN-125, 5033428E16Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 67405
VEGA: 10
HGNC: HGNC:8038
Homologene: 4506
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Kat2b
Name: K(lysine) acetyltransferase 2B
Synonyms: A930006P13Rik, Pcaf
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18519
HGNC: HGNC:8638
Homologene: 20834
Mbd4
Name: methyl-CpG binding domain protein 4
Synonyms: Med1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 17193
HGNC: HGNC:6919
Homologene: 2916
Kdm1a
Name: lysine (K)-specific demethylase 1A
Synonyms: 1810043O07Rik, LSD1, Aof2, Kdm1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 99982
Homologene: 32240
Tasp1
Name: taspase, threonine aspartase 1
Synonyms: 4930485D02Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 75812
Homologene: 9795
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 130,707,588 bp
  • C to T, chromosome 1 at 146,701,671 bp
  • G to A, chromosome 2 at 22,871,562 bp
  • A to T, chromosome 2 at 76,808,075 bp
  • T to C, chromosome 2 at 80,656,031 bp
  • A to C, chromosome 2 at 88,311,475 bp
  • G to T, chromosome 2 at 97,630,654 bp
  • A to G, chromosome 2 at 114,644,755 bp
  • A to T, chromosome 2 at 125,382,034 bp
  • T to C, chromosome 2 at 131,280,338 bp
  • G to T, chromosome 2 at 139,985,131 bp
  • C to A, chromosome 3 at 66,205,672 bp
  • T to A, chromosome 3 at 129,706,137 bp
  • T to A, chromosome 4 at 33,944,416 bp
  • A to G, chromosome 4 at 118,665,717 bp
  • T to C, chromosome 4 at 123,432,924 bp
  • C to T, chromosome 4 at 128,723,089 bp
  • C to T, chromosome 4 at 136,557,749 bp
  • A to T, chromosome 4 at 136,896,433 bp
  • A to T, chromosome 5 at 63,808,004 bp
  • T to C, chromosome 5 at 145,154,829 bp
  • A to T, chromosome 5 at 146,079,344 bp
  • G to A, chromosome 6 at 48,464,638 bp
  • C to T, chromosome 6 at 48,835,935 bp
  • G to A, chromosome 6 at 72,359,074 bp
  • A to T, chromosome 6 at 115,845,363 bp
  • T to A, chromosome 6 at 132,855,904 bp
  • T to A, chromosome 6 at 141,909,069 bp
  • G to A, chromosome 7 at 21,098,194 bp
  • G to A, chromosome 7 at 48,808,819 bp
  • A to G, chromosome 7 at 99,735,677 bp
  • A to C, chromosome 8 at 46,038,479 bp
  • T to C, chromosome 8 at 46,576,314 bp
  • C to T, chromosome 8 at 61,711,458 bp
  • C to T, chromosome 8 at 91,015,209 bp
  • A to T, chromosome 9 at 39,956,624 bp
  • C to A, chromosome 9 at 40,288,397 bp
  • C to A, chromosome 9 at 42,366,936 bp
  • C to T, chromosome 9 at 59,779,858 bp
  • C to T, chromosome 9 at 66,371,876 bp
  • A to T, chromosome 10 at 23,786,854 bp
  • G to A, chromosome 10 at 57,527,822 bp
  • T to C, chromosome 10 at 64,088,648 bp
  • T to C, chromosome 10 at 102,484,923 bp
  • T to C, chromosome 11 at 70,526,570 bp
  • C to G, chromosome 11 at 70,612,910 bp
  • G to T, chromosome 11 at 73,037,232 bp
  • A to T, chromosome 12 at 55,741,513 bp
  • T to A, chromosome 14 at 52,778,864 bp
  • T to A, chromosome 14 at 55,872,045 bp
  • A to T, chromosome 16 at 19,530,608 bp
  • T to G, chromosome 16 at 45,126,435 bp
  • A to T, chromosome 17 at 43,625,960 bp
  • A to G, chromosome 17 at 53,638,387 bp
  • T to C, chromosome 17 at 63,896,278 bp
  • A to G, chromosome 17 at 85,687,675 bp
  • T to C, chromosome 18 at 35,652,889 bp
  • C to A, chromosome 18 at 37,302,512 bp
  • T to C, chromosome 18 at 54,897,601 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7723 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045779-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.