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Strain Name:
C57BL/6J-MtgxR7818Btlr/Mmmh
Stock Number:
045872-MU
Citation ID:
RRID:MMRRC_045872-MU
Other Names:
R7818 (G1)
Major Collection:

Strain Information

Prph
Name: peripherin
Synonyms: Prph1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 19132
VEGA: 15
HGNC: HGNC:9461
Homologene: 4559
Lmf1
Name: lipase maturation factor 1
Synonyms: 2400010G15Rik, Tmem112
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 76483
Homologene: 23384
Slc1a2
Name: solute carrier family 1 (glial high affinity glutamate transporter), member 2
Synonyms: GLT1, MGLT1, Eaat2, 1700091C19Rik, GLT-1, 2900019G14Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20511
Homologene: 3075
Sez6
Name: seizure related gene 6
Synonyms: sez-6, D11Bhm177e
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20370
Homologene: 10948
Zmynd8
Name: zinc finger, MYND-type containing 8
Synonyms: 1110013E22Rik, 2010005I16Rik, ZMYND8, RACK7, 3632413B07Rik, Prkcbp1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228880
HGNC: HGNC:9397
Homologene: 32679
Spidr
Name: scaffolding protein involved in DNA repair
Synonyms: 2310008H04Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224008
VEGA: 16
Homologene: 51693
Dock1
Name: dedicator of cytokinesis 1
Synonyms: D630004B07Rik, 9130006G06Rik, Dock180, b2b3190Clo
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 330662
HGNC: HGNC:2987
Homologene: 55575
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 74,946,645 bp
  • C to T, chromosome 1 at 84,760,806 bp
  • T to C, chromosome 1 at 88,234,612 bp
  • T to C, chromosome 1 at 154,398,406 bp
  • G to A, chromosome 1 at 155,130,636 bp
  • T to C, chromosome 1 at 162,647,494 bp
  • A to G, chromosome 2 at 25,181,238 bp
  • A to G, chromosome 2 at 26,276,174 bp
  • T to C, chromosome 2 at 30,396,491 bp
  • A to G, chromosome 2 at 89,751,944 bp
  • A to G, chromosome 2 at 102,743,956 bp
  • G to A, chromosome 2 at 165,842,831 bp
  • A to T, chromosome 2 at 167,989,426 bp
  • A to T, chromosome 2 at 181,574,667 bp
  • G to T, chromosome 3 at 113,629,951 bp
  • A to T, chromosome 4 at 9,475,015 bp
  • A to G, chromosome 4 at 60,004,884 bp
  • T to C, chromosome 4 at 83,014,008 bp
  • T to A, chromosome 4 at 94,827,716 bp
  • C to T, chromosome 4 at 113,942,726 bp
  • A to T, chromosome 4 at 124,926,663 bp
  • C to A, chromosome 4 at 130,014,560 bp
  • C to T, chromosome 4 at 130,014,969 bp
  • T to A, chromosome 4 at 140,798,142 bp
  • G to A, chromosome 4 at 148,946,076 bp
  • T to A, chromosome 5 at 4,013,875 bp
  • A to G, chromosome 5 at 34,465,653 bp
  • T to A, chromosome 5 at 100,806,310 bp
  • T to C, chromosome 5 at 117,115,749 bp
  • T to A, chromosome 5 at 127,564,088 bp
  • A to T, chromosome 5 at 138,301,443 bp
  • C to A, chromosome 6 at 17,099,744 bp
  • T to C, chromosome 6 at 69,438,491 bp
  • G to A, chromosome 6 at 124,517,725 bp
  • C to G, chromosome 7 at 91,940,017 bp
  • G to A, chromosome 7 at 107,903,023 bp
  • A to G, chromosome 7 at 112,345,307 bp
  • A to G, chromosome 7 at 114,491,440 bp
  • T to A, chromosome 7 at 134,763,865 bp
  • T to C, chromosome 7 at 139,255,324 bp
  • A to T, chromosome 8 at 34,873,028 bp
  • G to A, chromosome 8 at 61,487,942 bp
  • G to A, chromosome 8 at 66,889,376 bp
  • G to T, chromosome 8 at 124,329,788 bp
  • T to A, chromosome 9 at 18,945,009 bp
  • T to A, chromosome 9 at 40,092,712 bp
  • T to A, chromosome 9 at 46,304,221 bp
  • C to T, chromosome 9 at 92,490,790 bp
  • T to C, chromosome 9 at 105,414,757 bp
  • C to A, chromosome 9 at 106,369,130 bp
  • A to T, chromosome 9 at 106,570,539 bp
  • A to C, chromosome 9 at 119,784,111 bp
  • A to G, chromosome 10 at 4,549,603 bp
  • T to C, chromosome 10 at 39,823,902 bp
  • A to G, chromosome 10 at 41,263,166 bp
  • T to A, chromosome 10 at 88,558,667 bp
  • C to T, chromosome 10 at 127,295,793 bp
  • A to G, chromosome 11 at 5,977,812 bp
  • A to T, chromosome 11 at 66,025,211 bp
  • C to T, chromosome 11 at 77,976,902 bp
  • A to G, chromosome 11 at 91,576,126 bp
  • T to C, chromosome 11 at 116,543,910 bp
  • A to T, chromosome 11 at 117,792,127 bp
  • C to T, chromosome 11 at 120,021,455 bp
  • C to T, chromosome 13 at 23,554,991 bp
  • T to A, chromosome 13 at 95,986,820 bp
  • G to T, chromosome 14 at 30,479,721 bp
  • A to T, chromosome 14 at 50,361,828 bp
  • A to T, chromosome 14 at 55,099,724 bp
  • G to T, chromosome 14 at 87,472,200 bp
  • T to C, chromosome 15 at 55,065,930 bp
  • A to G, chromosome 15 at 75,981,015 bp
  • A to G, chromosome 15 at 89,096,816 bp
  • A to G, chromosome 15 at 99,057,872 bp
  • T to C, chromosome 16 at 16,114,865 bp
  • A to C, chromosome 16 at 16,673,573 bp
  • A to G, chromosome 16 at 17,894,447 bp
  • T to A, chromosome 16 at 26,698,847 bp
  • T to A, chromosome 16 at 45,737,553 bp
  • A to T, chromosome 17 at 21,587,723 bp
  • G to T, chromosome 17 at 23,953,262 bp
  • T to G, chromosome 17 at 25,662,591 bp
  • A to T, chromosome 17 at 70,849,608 bp
  • A to T, chromosome 18 at 20,050,132 bp
  • T to C, chromosome 18 at 53,723,103 bp
  • G to C, chromosome 18 at 60,829,051 bp
  • T to A, chromosome 18 at 61,281,509 bp
  • A to G, chromosome 19 at 5,052,191 bp
  • A to C, chromosome 19 at 59,263,161 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7818 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045872-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.