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Strain Name:
C57BL/6J-MtgxR7821Btlr/Mmmh
Stock Number:
045875-MU
Citation ID:
RRID:MMRRC_045875-MU
Other Names:
R7821 (G1)
Major Collection:

Strain Information

Abca12
Name: ATP-binding cassette, sub-family A member 12
Synonyms: 4832428G11Rik, 4833417A11Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 74591
Homologene: 45441
Dst
Name: dystonin
Synonyms: bullous pemphigoid antigen 1, BPAG1-n, BPAG1, Bpag1, Bpag, ah, bullous pemphigoid antigen 1, athetoid, Macf2, 2310001O04Rik, nmf203, A830042E19Rik, nmf339
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Zmynd8
Name: zinc finger, MYND-type containing 8
Synonyms: 1110013E22Rik, 2010005I16Rik, ZMYND8, RACK7, 3632413B07Rik, Prkcbp1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228880
HGNC: HGNC:9397
Homologene: 32679
Ubr5
Name: ubiquitin protein ligase E3 component n-recognin 5
Synonyms: 4432411E13Rik, Edd1, Edd
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 70790
VEGA: 15
Homologene: 9295
Shroom3
Name: shroom family member 3
Synonyms: D5Ertd287e, Shrm, Shrm3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 27428
Homologene: 9263
Dhcr24
Name: 24-dehydrocholesterol reductase
Synonyms: 5830417J06Rik, seladin-1, 3-beta-hydroxysterol delta-24 reductase, 2310076D10Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 74754
HGNC: HGNC:2859
Homologene: 8850
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 34,275,362 bp
  • A to T, chromosome 1 at 59,067,792 bp
  • G to T, chromosome 1 at 71,259,791 bp
  • T to C, chromosome 2 at 32,026,905 bp
  • T to C, chromosome 2 at 52,434,508 bp
  • G to A, chromosome 2 at 69,394,438 bp
  • T to C, chromosome 2 at 147,085,288 bp
  • A to T, chromosome 2 at 165,881,080 bp
  • A to T, chromosome 3 at 50,381,027 bp
  • C to A, chromosome 3 at 110,250,987 bp
  • T to A, chromosome 3 at 138,226,375 bp
  • T to C, chromosome 4 at 46,344,578 bp
  • T to A, chromosome 4 at 58,179,601 bp
  • T to C, chromosome 4 at 106,571,436 bp
  • T to A, chromosome 5 at 34,710,209 bp
  • T to C, chromosome 5 at 90,592,888 bp
  • T to A, chromosome 5 at 92,940,846 bp
  • T to C, chromosome 5 at 105,812,344 bp
  • A to G, chromosome 5 at 138,276,297 bp
  • T to A, chromosome 6 at 23,120,930 bp
  • T to A, chromosome 6 at 48,905,811 bp
  • G to A, chromosome 6 at 87,110,897 bp
  • A to G, chromosome 6 at 128,948,777 bp
  • T to A, chromosome 6 at 130,185,600 bp
  • GGCG to GGCGACGGCTGCG, chromosome 7 at 97,579,906 bp
  • G to A, chromosome 7 at 104,278,426 bp
  • G to A, chromosome 7 at 105,765,145 bp
  • A to G, chromosome 7 at 127,530,327 bp
  • A to T, chromosome 7 at 141,442,280 bp
  • A to T, chromosome 8 at 44,950,224 bp
  • A to T, chromosome 8 at 69,300,235 bp
  • T to A, chromosome 8 at 70,769,764 bp
  • T to A, chromosome 8 at 124,343,395 bp
  • T to A, chromosome 9 at 37,644,900 bp
  • T to C, chromosome 9 at 96,580,484 bp
  • T to C, chromosome 9 at 105,864,259 bp
  • T to C, chromosome 9 at 110,630,252 bp
  • TGGTGCGTGAG to TG, chromosome 10 at 80,002,590 bp
  • T to C, chromosome 11 at 51,069,712 bp
  • A to G, chromosome 11 at 75,494,474 bp
  • A to G, chromosome 11 at 87,638,128 bp
  • A to C, chromosome 11 at 94,544,462 bp
  • C to G, chromosome 11 at 96,271,711 bp
  • A to G, chromosome 12 at 4,296,221 bp
  • A to G, chromosome 12 at 4,857,975 bp
  • A to T, chromosome 12 at 79,255,324 bp
  • A to G, chromosome 12 at 115,312,176 bp
  • T to A, chromosome 13 at 6,597,872 bp
  • C to A, chromosome 13 at 76,056,392 bp
  • A to G, chromosome 14 at 7,939,113 bp
  • A to T, chromosome 14 at 61,545,680 bp
  • A to G, chromosome 14 at 95,882,050 bp
  • T to C, chromosome 14 at 122,971,877 bp
  • T to C, chromosome 15 at 5,212,025 bp
  • T to A, chromosome 15 at 28,411,532 bp
  • A to T, chromosome 15 at 37,997,187 bp
  • G to A, chromosome 15 at 77,943,350 bp
  • T to A, chromosome 16 at 3,848,177 bp
  • T to A, chromosome 16 at 45,965,161 bp
  • T to C, chromosome 17 at 22,559,051 bp
  • A to G, chromosome 17 at 38,335,964 bp
  • A to G, chromosome 17 at 88,986,213 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7821 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045875-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.